Development of chemiluminescent enzyme immunoassay for the determination of malachite green in seafood

2014 ◽  
Vol 26 (2) ◽  
pp. 204-217 ◽  
Author(s):  
Yan Zhang ◽  
Jin-yi Yang ◽  
Hong-tao Lei ◽  
Hong Wang ◽  
Zhen-lin Xu ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Malachite Green ◽  
Chemiluminescent Enzyme Immunoassay

Determination of progesterone receptor by chemiluminescent enzyme immunoassay

2003 ◽  
Vol 23 (1) ◽  
pp. 60-61
Author(s):  
Yin Tiejun ◽  
Gu Meijiao ◽  
Zhou Yikai ◽  
Zheng Wen ◽  
Hu Wei ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

Determination of Free Triiodothyronine by Chemiluminescent Enzyme Immunoassay

2008 ◽  
Vol 36 (5) ◽  
pp. 609-614 ◽  
Author(s):  
Zhen LIN ◽  
Xu WANG ◽  
Shi-Qi REN ◽  
Guo-Nan CHEN ◽  
Zhen-Jia LI ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Free Triiodothyronine ◽  
Chemiluminescent Enzyme Immunoassay

Development of sensitive direct chemiluminescent enzyme immunoassay for the determination of dihydroartemisinin in plasma

2015 ◽  
Vol 407 (25) ◽  
pp. 7823-7830 ◽  
Author(s):  
Laura Zehnacker ◽  
Marie-Claire Nevers ◽  
Véronique Sinou ◽  
Dominique Parzy ◽  
Christophe Créminon ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Quantitative analysis of chloramphenicol residues in shrimp muscle tissues by Chemiluminescent enzyme immunoassay

2011 ◽  
Vol 23 (No. 6) ◽  
pp. 251-256
Author(s):  
X. Chuanlai ◽  
P. Cifang ◽  
H. Kai ◽  
J. Zhengyu ◽  
W. Wukang
Keyword(s):  
Enzyme Immunoassay ◽  
Limit Of Detection ◽  
Tween 20 ◽  
Detection Range ◽  
Muscle Tissues ◽  
Physico Chemical ◽  
Interassay Coefficient ◽  
Chemiluminescent Enzyme Immunoassay ◽  
Better Than

A competitive indirect chemiluminescent enzyme immunoassay (ic-CLEIA) has been developed for the determination of chloramphenicol (CAP) residues in shrimp. After the optimisation of four physico-chemical parameters, i.e. incubation time, concentration of Tween-20, concentration of PBS and its pH, the method developed gave a limit of detection of 0.01 ng/ml and a detection range from 0.03 ng/ml to 23.7 ng/ml, with an ED<sub>50</sub> of 0.47 ng/ml. The developed method has been validated on spiked shrimp samples in terms of precision (intra- and interassay coefficient variations of less than 10% and 15%, respectively), and of accuracy (mean recovery from 95% to 123%). All these parameters being better than those of the ELISA method which is widely used to detect chloramphenicol, it may be suggested that the CLEIA method can be used to detect aquatic samples instead of ELISA. &nbsp;

Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milk

2012 ◽  
Vol 4 (8) ◽  
pp. 2550 ◽  
Author(s):  
Marina M. Vdovenko ◽  
Anastasia V. Gribas ◽  
Alexandra V. Vylegzhanina ◽  
Ivan Yu. Sakharov
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Development of chemiluminescent enzyme immunoassay for the determination of aflatoxin M1in milk products

2014 ◽  
Vol 26 (2) ◽  
pp. 157-169 ◽  
Author(s):  
Ping Li ◽  
Yan Zhang ◽  
Hong-tao Lei ◽  
Hong Wang ◽  
Zhen-lin Xu ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Milk Products ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Determination of Enrofloxacin in Bovine Milk by a Novel Single-Stranded DNA Aptamer Chemiluminescent Enzyme Immunoassay

2014 ◽  
Vol 47 (17) ◽  
pp. 2844-2856 ◽  
Author(s):  
Hengjia Ni ◽  
Suxia Zhang ◽  
Xinghua Ding ◽  
Tiejun Mi ◽  
Zhanhui Wang ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Bovine Milk ◽  
Dna Aptamer ◽  
Single Stranded Dna ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 305
Author(s):  
Long Xu ◽  
Xiao-yi Suo ◽  
Qi Zhang ◽  
Xin-ping Li ◽  
Chen Chen ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Quantitative Measurement ◽  
High Sensitivity ◽  
Health Concerns ◽  
Specific Determination ◽  
Food And Feed ◽  
Sensitivity Specificity ◽  
Feed Samples ◽  
Chemiluminescent Enzyme Immunoassay

Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC50) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%–108.3% (ic-ELISA) and 80.1%–98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy.

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