<p><strong>Objective</strong> To establish the 3rd national reference standard for Tachypleus Tridentatus Lysate Reagent. <strong>Method</strong> <strong>and Results</strong> The candidates of reference standard were studied for the physical and chemical properties, and the stability. To determined its sensitivity by collaboration calibration. <strong>Conclusions</strong> The sensitivity of the 3rd national reference standard for TAL is 0.06EU / ml, lot number is 150603-201003.</p>