scholarly journals In Vitro Regeneration of ICP 8863 Pigeon Pea (Cajanus cajan (L.) Millsp.) Variety using Leaf Petiole and Cotyledonary Node Explants and Assessment of their Genetic Stability by RAPD Analysis

2019 ◽  
Vol 12 (9) ◽  
pp. 1-10
Author(s):  
Nirmala Nalluri ◽  
Vasavi Rama Karri ◽  
◽  
Keyword(s):  
Genetic Stability ◽  
Cajanus Cajan ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Cotyledonary Node ◽  
Pigeon Pea ◽  
Leaf Petiole ◽  
Cotyledonary Node Explants

scholarly journals In vitro regeneration and somatic embryogenesis in citrus

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Rt Pcr ◽  
Mother Plants

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

scholarly journals In Vitro Regeneration of Pigeonpea [Cajanus cajan (L.) Millsp.] Genotype GT 101 using Cotyledonary Node

2016 ◽  
Vol 9 (45) ◽  
Author(s):  
Hitesh V. Jasani ◽  
Nimita G. Umretiya ◽  
Manthan N. Kapuria ◽  
Darshan T. Dharajiya ◽  
Tarun J. Khatrani ◽  
...  
Keyword(s):  
Cajanus Cajan ◽  
In Vitro Regeneration ◽  
Cotyledonary Node

scholarly journals In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

2011 ◽  
Vol 17 (4) ◽  
pp. 375-385 ◽  
Author(s):  
Gaurav Krishna ◽  
P. Sairam Reddy ◽  
Pramod W. Ramteke ◽  
Pogiri Rambabu ◽  
Sayed S. Sohrab ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Cajanus Cajan ◽  
In Vitro Regeneration ◽  
Pigeon Pea

scholarly journals Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa

2004 ◽  
Vol 39 (2) ◽  
pp. 197-199 ◽  
Author(s):  
Rachel Fatima Gagliardi ◽  
Georgia Pereira Pacheco ◽  
Carlos Alberto Oliveira ◽  
Leonardo Alves Carneiro ◽  
José Francisco Montenegro Valls ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Preservation ◽  
Arachis Species ◽  
Embryo Axes ◽  
Genomic Regions ◽  
Regeneration Processes

In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.

Sign in / Sign up

Export Citation Format

Share Document