scholarly journals Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa

2004 ◽  
Vol 39 (2) ◽  
pp. 197-199 ◽  
Author(s):  
Rachel Fatima Gagliardi ◽  
Georgia Pereira Pacheco ◽  
Carlos Alberto Oliveira ◽  
Leonardo Alves Carneiro ◽  
José Francisco Montenegro Valls ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Preservation ◽  
Arachis Species ◽  
Embryo Axes ◽  
Genomic Regions ◽  
Regeneration Processes

In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.

scholarly journals In vitro regeneration and somatic embryogenesis in citrus

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Rt Pcr ◽  
Mother Plants

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Sunil Senapati ◽  
Subhashree Aparajita ◽  
Gyana Rout
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Endangered Medicinal Plant ◽  
Celastrus Paniculatus

AbstractA highly efficient protocol for in vitro regeneration of an indigenous, endangered medicinal plant Celastrus paniculatus was achieved using nodal explants. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants. Maximum rooting of 73.3% with 4.8 roots/shoot was achieved on half-strength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker study confirmed genetic stability for in vitro raised explants by showing 100% monomorphism. High multiplication rate associated with genetic stability ensure the efficacy of the present in vitro clonal propagation protocol of this important medicinal plant species.

Genetic Stability of Micropropagated Ginger Derived from Axillary Bud through Cytophotometric and RAPD Analysis

2008 ◽  
Vol 63 (9-10) ◽  
pp. 747-754 ◽  
Author(s):  
Sujata Mohanty ◽  
Manoj K. Panda ◽  
Enketeswara Subudhi ◽  
Laxmikanta Acharya ◽  
Sanghamitra Nayak
Keyword(s):  
Dna Content ◽  
Genetic Stability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Nuclear Dna Content ◽  
Basal Medium ◽  
Axillary Buds ◽  
Axillary Bud ◽  
Multiplication Rate

A protocol was developed for the in vitro propagation of ginger (Zingiber officinale) cv. Suprava using dormant axillary buds from unsprouted rhizomes. The dormant axillary buds embedded in the rhizome nodes were induced to sprout when cultured on MS medium supplemented with 6-benzyladenine (BA) alone (1 - 6 mg/l) or with a combination of BA (1 - 6 mg/l) and indole-3-acetic acid (IAA) (0.5, 1 mg/l). In vitro sprouted buds were transferred to the multiplication medium containing various combinations of auxins and cytokinins. MS basal medium supplemented with BA (1 mg/l), IAA (1 mg/l) and adenine sulfate (100 mg/l) was found optimum for the in vitro multiplication of shoots producing (8.2 ± 0.2) shoots from a single explant within 30 days of culture. The multiplication rate remained unchanged in subsequent subcultures. Rooting of shoots occurred in the same multiplication media. Upon transfer of the in vitro culture to ex vitro in pots, 96% of plants survived and established successfully under natural conditions. Tissue culture-raised plantlets of ginger could be conserved in vitro through subculturing at an interval of 4 months. The genetic stability of micropropagated clones was evaluated at regular intervals of 6 months up to 24 months in culture using cytophotometric estimation of 4C nuclear DNA content and random amplified polymorphic DNA (RAPD) analysis. Cytophotometric analysis revealed a unimodal distribution of the DNA content with a peak corresponding to the 4C value (23.1 pg), and RAPD analysis revealed monomorphic bands showing the absence of polymorphism in all fifty regenerants analyzed, thus confirming the genetic uniformity among in vitro grown somaclones of Z. officinale. This study is of commercial significance as axillary bud explants are available throughout the year for initiating a fresh culture of the elite ginger cv. Suprava to be used as a source of true-to-type disease-free planting material thereby minimizing the adverse effect of repeated subculturing from the same explant source.

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

scholarly journals In vitro regeneration and PCR-RAPD based detection of somaclonal variation in kenaf (Hibiscus cannabinus)

2017 ◽  
Vol 28 (2) ◽  
pp. 100-108
Author(s):  
MS Haque ◽  
T Biswas ◽  
MS Islam ◽  
MS Hossain
Keyword(s):  
Somaclonal Variation ◽  
Rapd Markers ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Hibiscus Cannabinus ◽  
Petiole Explants ◽  
Napthaleneacetic Acid ◽  
Mother Plants ◽  
Regenerated Plantlets

Though direct systems of regeneration through culture of organized meristems usually produce true-to-type plants, variations in the progenies have widely been reported. Fiber producing kenaf plants (Hibiscus cannabinus L.) were regenerated from petiole, hypocotyls and cotyledonous petiole explants on MS medium containing BAP (benzyl amino purine) and NAA (?-napthaleneacetic acid) followed by assessment of regenerants by RAPD markers to detect somaclonal variation among them. Genomic DNA from twenty seven plants [three mother plants and two clones (clone 1 and 2) from each mother plant with three replications] was subjected to random amplified polymorphic DNA (RAPD) analysis. Fifteen polymorphic loci amplified by three decamer random primers were used to estimate genetic diversity and relatedness in mother plants and their regenerated plantlets. The results showed some degree of polymorphism between mother plants and their regenerated plantlets as well as between regenerated plantlets indicating somaclonal variation among the regenerants. These suggest that the RAPD technique could effectively be used to detect somaclonal variation in H. cannabinus and could be promising for the detection of markers associated with desirable traits.Progressive Agriculture 28 (2): 100-108, 2017

scholarly journals Detection of Genetic Variation among Camellia Parents and Hybrids Using Random Amplified Polymorphic DNA (RAPD) Markers

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1121C-1121
Author(s):  
Lianghong Chen ◽  
Shizhou Wang ◽  
Mack Nelson
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Approach ◽  
Random Primers ◽  
Genomic Regions ◽  
Genotype A

The reliability of the random amplified polymorphic DNA (RAPD) technique in amplifying polymorphisim among the hybrids and their parents' genomes of the genus Camellia was evaluated. Three hybrids (`Londontowne Blush', `Ashton's Snow', and `Ashton's Cameo') and one of the parents, C. oleifrea`Plain Jane', provided by the America Camellia Society, Fort Valley, Ga., were investigated. Twenty 10-based random primers were tested in this study. Five out of 20 primers were selected for RAPD analysis based on the ability to produce unambiguously scoreable RAPD bands for evaluation and comparison of the genotypes under investigation. The five primers were selected because they produced distinct patterns of amplified bands for each tested genotype. A total of 162 RAPD bands were produced. Among the 162 bands, 86 bands showed polymorphisms. The amplified band sizes ranged from 236 to 1656 bp. These data indicate that in the three hybrids and one of the parents exist unique genomic regions. Our investigation results showed that the RAPD molecular approach can be used to discriminate genetic variation among hybrids and their parents.

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