Anti-inflammatory Effects of Fermented Laminaria japonica and Hizikia fusiforme Water Extracts with Probiotics in LPS-stimulated RAW264.7 Macrophage Cell Line

2017 ◽  
Vol 27 (1) ◽  
pp. 1-8
Author(s):  
Yeon-ji Hwang ◽  
Insook Chae ◽  
Yunkyoung Lee
Keyword(s):  
Cell Line ◽  
Laminaria Japonica ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Water Extracts ◽  
Hizikia Fusiforme ◽  
Anti Inflammatory ◽  
Raw264.7 Macrophage

scholarly journals Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line

2017 ◽  
Vol 12 (1) ◽  
pp. 35 ◽  
Author(s):  
Dian Ratih Laksmitawati ◽  
Anisa Widyastuti ◽  
Nadia Karami ◽  
Ervi Afifah ◽  
Dwi Davidson Rihibiha ◽  
...  
Keyword(s):  
Cell Line ◽  
Tumor Necrosis ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Α Level ◽  
Necrosis Factor

<p class="Abstract">In this study, the anti-inflammatory potential of <em>Anredera </em>cordifolia and <em>Piper </em>crocatum extracts on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7) was observed. Cell viability assay was performed with MTS assay. Parameters measured to determine the anti-inflammatory activity were interleukin-1β (IL-1β), tumor necrosis factor (TNF)-α, nitric oxide (NO) and IL-6. Both <em>A. </em>cordifolia and<em> P. </em>crocatum at concentration of 50 µg/mL in cell line resulted significant decrease in TNF-α level (250.3 and 242.5 pg/mL respectively). <em>A. </em>cordifolia showed significant decrease in IL-1β level at 50 µg/mL and IL-6 level at 10 µg/mL, whilst <em>P. </em>crocatum  showed significant decrease IL-1β level in three concentrations with lowest level at 50 µg/mL.<em> A. </em>cordifolia showed lowest decrease in NO level at 50 µg/mL but not comparable with normal cells, whilst <em>P. </em>crocatum showed significant decrease in NO level at 50 µg/mL. This research revealed that <em>A. </em>cordifolia and<em> P. </em>crocatum possess the anti-inflammatory potential indicated by the inhibitory activity of the inflammatory mediators including, TNF-α, IL-1β, IL-6, and NO.</p>

WNT/β-catenin pathway is modulated in asthma patients and LPS-stimulated RAW264.7 macrophage cell line

2011 ◽  
Vol 34 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Haeyong Lee ◽  
Sungmin Bae ◽  
Byoung Whui Choi ◽  
Yoosik Yoon
Keyword(s):  
Cell Line ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Asthma Patients ◽  
Raw264.7 Macrophage

scholarly journals Suppression of methylmercury-induced MIP-2 expression by N-acetyl-l-cysteine in murine RAW264.7 macrophage cell line

2017 ◽  
Vol 22 (1) ◽  
Author(s):  
Juliet David ◽  
Athira Nandakumar ◽  
Muflihatul Muniroh ◽  
Suminori Akiba ◽  
Megumi Yamamoto ◽  
...  
Keyword(s):  
Cell Line ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Raw264.7 Macrophage

scholarly journals Lipopolysaccharide induces calcitonin gene-related peptide in the RAW264.7 macrophage cell line

Immunology ◽  
2010 ◽  
Vol 130 (3) ◽  
pp. 399-409 ◽  
Author(s):  
Weiya Ma ◽  
Yvan Dumont ◽  
Freya Vercauteren ◽  
Remi Quirion
Keyword(s):  
Cell Line ◽  
Calcitonin Gene Related Peptide ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Raw264.7 Macrophage

Anti-inflammatory activities of red curry paste extract on lipopolysaccharide-activated murine macrophage cell line

Nutrition ◽  
2011 ◽  
Vol 27 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Siriporn Tuntipopipat ◽  
Channarong Muangnoi ◽  
Pimjai Chingsuwanrote ◽  
Montira Parengam ◽  
Pranom Chantravisut ◽  
...  
Keyword(s):  
Cell Line ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Anti Inflammatory

Chemotaxis of Lipopolysaccharide-Stimulated RAW264.7 Macrophage Cell Line in Microfluidic Channels

2017 ◽  
Vol 17 (11) ◽  
pp. 7996-8000
Author(s):  
Junhyun Park ◽  
Jayoung Koo ◽  
Ji-Youn Kim ◽  
Kyung-Jin Chung ◽  
Eun-Surk Yi ◽  
...  
Keyword(s):  
Cell Line ◽  
Microfluidic Channels ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Raw264.7 Macrophage

scholarly journals Elucidation of the Mechanism Underlying the Anti-Inflammatory Properties of (S)-(+)-Carvone Identifies a Novel Class of Sirtuin-1 Activators in a Murine Macrophage Cell Line

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 777
Author(s):  
Cátia Sousa ◽  
Bruno Miguel Neves ◽  
Alcino Jorge Leitão ◽  
Alexandrina Ferreira Mendes
Keyword(s):  
Cell Line ◽  
Sirtuin 1 ◽  
Low Grade ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Protein Levels ◽  
Age Related ◽  
Anti Inflammatory

The signaling pathways involved in age-related inflammation are increasingly recognized as targets for the development of preventive and therapeutic strategies. Our previous study elucidated the structure–activity relationship of monoterpene compounds derived from p-menthane as potential anti-inflammatory drugs and identified (S)-(+)-carvone as the most potent among the compounds tested. This study aims at identifying the molecular mechanism underlying the anti-inflammatory properties of (S)-(+)-carvone. The murine macrophage cell line, Raw 264.7, was stimulated with bacterial lipopolysaccharide (LPS) to simulate inflammation. Western blot was used to assess protein levels and post-translational modifications. The subcellular localization of NF-κB/p65 was visualized by immunocytochemistry. An in vitro fluorometric assay was used to measure Sirtuin-1 (SIRT1) activity. (S)-(+)-carvone inhibited LPS-induced JNK1 phosphorylation, but not that of p38 and ERK1/2 and also did not affect the phosphorylation and degradation of the NF-κB inhibitor, IκB-α. Accordingly, (S)-(+)-carvone did not affect LPS-induced phosphorylation of NF-κB/p65 on Ser536 and its nuclear translocation, but it significantly decreased LPS-induced IκB-α resynthesis, a NF-κB-dependent process, and NF-κB/p65 acetylation on lysine (Lys) 310. Deacetylation of that Lys residue is dependent on the activity of SIRT1, which was found to be increased by (S)-(+)-carvone, while its protein levels were unaffected. Taken together, these results show that (S)-(+)-carvone is a new SIRT1 activator with the potential to counteract the chronic low-grade inflammation characteristic of age-related diseases.

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