Canine/feline serum or plasma deproteinization for amino acid analysis on Biochrom v1

2019 ◽  
Author(s):  
Amanda Blake
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Internal Standard ◽  
Amino Acid Analyzer ◽  
Plasma Research

This protocol describes the deproteinization process that all canine and feline serum and plasma research samples undergo prior to amino acid analysis with a Biochrom 30+ Amino Acid Analyzer at the Gastrointestinal Lab, Texas A&M University. L-norleucine is used as an internal standard.

scholarly journals Urinary amino acid analysis: A comparison of iTRAQ®–LC–MS/MS, GC–MS, and amino acid analyzer

2009 ◽  
Vol 877 (20-21) ◽  
pp. 1838-1846 ◽  
Author(s):  
Hannelore Kaspar ◽  
Katja Dettmer ◽  
Queenie Chan ◽  
Scott Daniels ◽  
Subodh Nimkar ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Amino Acid Analyzer ◽  
Urinary Amino

A unique algorithm for the determination of peptide-carrier protein conjugation ratio by amino acid analysis using intrinsic internal standard

Vaccine ◽  
2020 ◽  
Vol 38 (29) ◽  
pp. 4507-4511
Author(s):  
Gengcheng J. Yang ◽  
Yanhong Yang ◽  
Andrew Shaddeau ◽  
Cindy X. Cai ◽  
Yile Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Internal Standard ◽  
Carrier Protein ◽  
Protein Conjugation ◽  
Peptide Carrier

Nitrotyrosine internal standard for amino acid analysis

1975 ◽  
Vol 64 (2) ◽  
pp. 588-592 ◽  
Author(s):  
Roger W. Giese ◽  
James F. Riordan
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Internal Standard

scholarly journals Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Almut Hesse ◽  
Michael G. Weller
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Limit Of Detection ◽  
Certified Reference Materials ◽  
Internal Standard ◽  
Aromatic Amino Acids ◽  
Protein Quantification ◽  
Protein Determination ◽  
Relative Standard

Amino acid analysis is considered to be the gold standard for quantitative peptide and protein analysis. Here, we would like to propose a simple HPLC/UV method based on a reversed-phase separation of the aromatic amino acids tyrosine (Tyr), phenylalanine (Phe), and optionally tryptophan (Trp) without any derivatization. The hydrolysis of the proteins and peptides was performed by an accelerated microwave technique, which needs only 30 minutes. Two internal standard compounds, homotyrosine (HTyr) and 4-fluorophenylalanine (FPhe) were used for calibration. The limit of detection (LOD) was estimated to be 0.05 µM (~10 µg/L) for tyrosine and phenylalanine at 215 nm. The LOD for a protein determination was calculated to be below 16 mg/L (~300 ng BSA absolute). Aromatic amino acid analysis (AAAA) offers excellent accuracy and a precision of about 5% relative standard deviation, including the hydrolysis step. The method was validated with certified reference materials (CRM) of amino acids and of a pure protein (bovine serum albumin, BSA). AAAA can be used for the quantification of aromatic amino acids, isolated peptides or proteins, complex peptide or protein samples, such as serum or milk powder, and peptides or proteins immobilized on solid supports.

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