[4] Amino acid analysis of proteins and peptides at the picomole level: The fluorescamine amino acid analyzer

1981 ◽  
pp. 20-25 ◽  
Author(s):  
Stanley Stein ◽  
Larry Brink
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Amino Acid Analyzer ◽  
Proteins And Peptides

scholarly journals Urinary amino acid analysis: A comparison of iTRAQ®–LC–MS/MS, GC–MS, and amino acid analyzer

2009 ◽  
Vol 877 (20-21) ◽  
pp. 1838-1846 ◽  
Author(s):  
Hannelore Kaspar ◽  
Katja Dettmer ◽  
Queenie Chan ◽  
Scott Daniels ◽  
Subodh Nimkar ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Amino Acid Analyzer ◽  
Urinary Amino

Canine/feline serum or plasma deproteinization for amino acid analysis on Biochrom v1

2019 ◽  
Author(s):  
Amanda Blake
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Internal Standard ◽  
Amino Acid Analyzer ◽  
Plasma Research

This protocol describes the deproteinization process that all canine and feline serum and plasma research samples undergo prior to amino acid analysis with a Biochrom 30+ Amino Acid Analyzer at the Gastrointestinal Lab, Texas A&M University. L-norleucine is used as an internal standard.

An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

1991 ◽  
Vol 56 (4) ◽  
pp. 923-932
Author(s):  
Jana Stejskalová ◽  
Pavel Stopka ◽  
Zdeněk Pavlíček
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Peroxidase Activity ◽  
Amino Acid Analysis ◽  
Superoxide Radical ◽  
Esr Spectra ◽  
The Other ◽  
Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

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