Combined Acetylene Blockage and Reduction Assay (COBRA) for Dinitrogen Fixation and Denitrification Estimation v1 (protocols.io.9ith4en)

protocols.io ◽  
2019 ◽  
Author(s):  
Yusuf Christian ◽  
Florian Roth ◽  
Nils R ◽  
Najeh Kharbatia ◽  
Burton H ◽  
...  
Keyword(s):  
Dinitrogen Fixation ◽  
Reduction Assay ◽  
Acetylene Blockage

Bacterial dinitrogen fixation in the leaf of the northern pitcher plant (Sarracenia purpurea)

1991 ◽  
Vol 69 (10) ◽  
pp. 2296-2298 ◽  
Author(s):  
Andrew B. Prankevicius ◽  
Duncan M. Cameron
Keyword(s):  
Key Words ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Dinitrogen Fixation ◽  
Acetylene Reduction Assay ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Physiological Conditions ◽  
Reduction Assay ◽  
Unidentified Source

Bacterial (Azotobacteriaceae) nitrogenase activity in the leaf cavity of the insectivorous northern pitcher plant (Sarracenia purpurea L.) was assessed by acetylene reduction assay. Results indicated that bacterial dinitrogen fixation occurs in the leaf cavity under normal physiological conditions. This may represent a previously unidentified source of nitrogen for the pitcher plant, as the level of nitrogenase activity measured was sufficient to provide the plant with nitrogen well in excess of that required. Key words: pitcher plant, Sarracenia purpurea, dinitrogen fixation, Azotobacteriaceae, nitrogenase.

scholarly journals Acetylene reduction (dinitrogen fixation) by clinical isolates of Klebsiella pneumoniae

1977 ◽  
Vol 6 (5) ◽  
pp. 456-460
Author(s):  
C A Chambers ◽  
W S Silver
Keyword(s):  
Klebsiella Pneumoniae ◽  
Optimum Condition ◽  
Yeast Extract ◽  
Acetylene Reduction ◽  
Incubation Temperature ◽  
Dinitrogen Fixation ◽  
Clinical Strains ◽  
Cultural Conditions ◽  
High Incidence ◽  
Reduction Assay

Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms were diazotrophs, whereas this temperature without yeast extract yielded 12.9% positive cultures. The optimum condition found was 28 degrees C without yeast extract (21.9% positive); therefore, search for diazotrophy in clinical strains should not be conducted at the usual incubation temperature. There was a high incidence of indole-positive strains among diazotrophs. No such correlation was noted with any other biochemical trait or antibiotic susceptibility tested. The significance of this correlation is not apparent.

Dinitrogen Fixation Technologies for Alfalfa Improvement

jpa ◽  
1989 ◽  
Vol 2 (1) ◽  
pp. 24-32 ◽  
Author(s):  
G. H. Heichel ◽  
D. K. Barnes ◽  
C. P. Vance ◽  
C. C. Sheaffer
Keyword(s):  
Dinitrogen Fixation

Rapid Nitrate Reduction Assay with Intact Microbial Cells or Spores

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (12) ◽  
Author(s):  
Marco Fischer ◽  
Dörte Falke ◽  
R. Sawers
Keyword(s):  
Nitrate Reduction ◽  
Microbial Cells ◽  
Reduction Assay

Mineral nutrition, dinitrogen fixation, and growth of Alnuscrispa and Alnusglutinosa

1985 ◽  
Vol 15 (5) ◽  
pp. 855-861 ◽  
Author(s):  
G. Prégent ◽  
C. Camiré
Keyword(s):  
Mineral Nutrition ◽  
Dry Weight ◽  
Optimal Growth ◽  
Maximum Growth ◽  
Dinitrogen Fixation ◽  
Weight Basis ◽  
Critical Levels ◽  
N Status

Invitro cultures of Alnuscrispa (Ait.) Pursh and Alnusglutinosa (L.) Gaertn. were used to estimate critical foliage levels of selected nutrients for optimal growth and dinitrogen (N2) fixation. For A. crispa to obtain 90% of maximum growth and N2 fixation, foliar levels of 0.12% P, 0.13% Mg, <0.31% K, and <0.04% Ca on a dry weight basis were needed. For A. glutinosa, the critical levels were 0.138% P, 0.10% Mg, 0.29% Ca, and ~0.20% K. From all the deficiencies observed, P had the more pronounced effects on N status of both species.

scholarly journals Factors influencing microbial mat composition, distribution and dinitrogen fixation in three western Indian Ocean coral reefs

2012 ◽  
Vol 47 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Loïc Charpy ◽  
Katarzyna A. Palinska ◽  
Raeid M. M. Abed ◽  
Marie José Langlade ◽  
Stjepko Golubic
Keyword(s):  
Indian Ocean ◽  
Coral Reefs ◽  
Western Indian Ocean ◽  
Dinitrogen Fixation ◽  
Microbial Mat ◽  
Composition Distribution ◽  
Factors Influencing
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