Agarose Gel Electrophoresis for quality control of DNA aptamers in biosensor assays v1

2021 ◽  
Author(s):  
Geisianny AM Moreira ◽  
Diana Vanegas ◽  
Eric S McLamore
Keyword(s):  
Quality Control ◽  
Gel Electrophoresis ◽  
Dna Aptamers ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Fundamental Step ◽  
Complete Process

This protocol describes the procedure for electrophoresis in agarose gel to check the quality, purity, and size of DNA aptamers. The general objective is that this is the first and fundamental step to start aptamer loading on LIG electrodes.The complete process requires approximately 2 hours and 15 minutes to complete.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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