Recruitments of Sherpa highlanders and non-Sherpa lowlanders v1

2021 ◽  
Author(s):  
Yunden Droma ◽  
not provided Masayuki Hanaoka ◽  
not provided Masao Ota
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Altitude ◽  
Sea Level ◽  
Venous Blood ◽  
Genetic Adaptation ◽  
Nucleotide Polymorphisms ◽  
Research Project ◽  
Single Nucleotide ◽  
Altitude Hypoxia ◽  
High Altitude Hypoxia

In order to carry out the research project of genetic adaptation to high-altitude hypoxia in Sherpa highlanders, we recruited Sherpa highlanders in Namche Bazaar village at a high altitude of 3,440 meters (m) above sea level and non-Sherpa lowlanders in Kathmandu city at 1,300 m in Nepal. Venous blood was sampled to obtain plasma and extract DNA in each subject. The concentrations of factors in plasma were measured. The single-nucleotide polymorphisms (SNPs) in the hypoxia-associated genes were genotyped.

scholarly journals Down-Regulation ofEPAS1Transcription and Genetic Adaptation of Tibetans to High-Altitude Hypoxia

2017 ◽  
pp. msw280 ◽  
Author(s):  
Yi Peng ◽  
Chaoying Cui ◽  
Yaoxi He ◽  
Ouzhuluobu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
High Altitude ◽  
Genetic Adaptation ◽  
Down Regulation ◽  
Altitude Hypoxia ◽  
High Altitude Hypoxia

scholarly journals Association of single-­nucleotide polymorphisms rs10867772, rs4700290 with sudden cardiac death

2021 ◽  
Vol 17 (1) ◽  
pp. 7-11
Author(s):  
A. A. Ivanova ◽  
S. K. Malyutina ◽  
V. P. Novoselov ◽  
I. A. Rodina ◽  
O. V. Khamovich ◽  
...  
Keyword(s):  
Sudden Cardiac Death ◽  
Single Nucleotide Polymorphisms ◽  
Cardiac Death ◽  
Statistical Significance ◽  
Venous Blood ◽  
Molecular Genetic ◽  
Control Group ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Genotype Frequencies

The aim of the research is to verify the association with sudden cardiac death (SCD) of single nucleotide polymorphisms rs10867772 and rs4700290, identified as new molecular genetic markers of SCD in the own genome-wide pooled allelotyping.Material and methods. Case-control study. The SCD group is formed using the criteria of the European Society of Cardiology from the DNA bank of suddenly deceased residents of the Oktyabrsky district of Novosibirsk (n = 437, average age—53.1 ± 9.0 years, men — 73.5%, women — 26.5%) The control group (n = 405, average age 53.2 ± 9.2 years, men — 70.0%, women — 30.0%) is formed from the DNA bank of participants of MONICA and HAPIEE projects. DNA was isolated by phenol-­chloroform extraction from myocardial tissue in the SCD group and venous blood in the control group. Genotyping was performed by the PCR-RFLP method.Results. No statistical significance was found in allele and genotype frequencies of rs10867772 and rs4700290 between groups, even in separating in sex and age (p> 0.05). Conclusion. Single nucleotide polymorphism rs10867772 and rs4700290 are not associated with SCD.

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