3’ mRNA-sequencing v1

2021 ◽  
Author(s):  
Bryan Yoo ◽  
Jessica Griffiths ◽  
Sarkis Mazmanian
Keyword(s):  
Mrna Sequencing

Protocol for RNAseq used in Yoo et al 2021

scholarly journals A Revised and Improved Version of the Northern Wheatear (Oenanthe oenanthe) Transcriptome

Diversity ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 151
Author(s):  
Roberto Carlos Frias-Soler ◽  
Lilian Villarín Pildaín ◽  
Michael Wink ◽  
Franz Bairlein
Keyword(s):  
Quality Parameters ◽  
Northern Wheatear ◽  
Long Distance ◽  
Assembly Quality ◽  
Oenanthe Oenanthe ◽  
Mrna Sequencing ◽  
Sequencing Coverage ◽  
Muscle Tissues ◽  
Migratory Season ◽  
Migratory Phenotype

This work presents an updated and more complete version of the transcriptome of a long-distance migrant, the Northern Wheatear (Oenanthe oenanthe). The improved transcriptome was produced from the independent mRNA sequencing of adipose tissue, brain, intestines, liver, skin, and muscle tissues sampled during the autumnal migratory season. This new transcriptome has better sequencing coverage and is more representative of the species’ migratory phenotype. We assembled 20,248 transcripts grouped into 16,430 genes, from which 78% were successfully annotated. All the standard assembly quality parameters were improved in the second transcriptome version.

scholarly journals Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition

2011 ◽  
Vol 21 (8) ◽  
pp. 1328-1338 ◽  
Author(s):  
Håvard Aanes ◽  
Cecilia L. Winata ◽  
Chi Ho Lin ◽  
Jieqi P. Chen ◽  
Kandhadayar G. Srinivasan ◽  
...  
Keyword(s):  
Mrna Sequencing ◽  
Maternal To Zygotic Transition

Dideoxy mRNA Sequencing as a Method to Analyze Antibody Diversity

1985 ◽  
pp. 141-142
Author(s):  
Gillian M. Griffiths ◽  
Claudia Berek ◽  
Matti Kaartinen ◽  
Cesar Milstein
Keyword(s):  
Antibody Diversity ◽  
Mrna Sequencing

scholarly journals BloodSpot: a database of healthy and malignant haematopoiesis updated with purified and single cell mRNA sequencing profiles

2018 ◽  
Vol 47 (D1) ◽  
pp. D881-D885 ◽  
Author(s):  
Frederik Otzen Bagger ◽  
Savvas Kinalis ◽  
Nicolas Rapin
Keyword(s):  
Single Cell ◽  
Mrna Sequencing

scholarly journals Single Endothelial Cell mRNA Sequencing Better Captures the Severity of Coronary Artery Disease Than Targeted Total Arterial Markers of Inflammation and Senescence

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Pauline Mury ◽  
Florian Wünnemann ◽  
Mélissa Beaudoin ◽  
Nathalie Thorin-Trescases ◽  
Yves Hébert ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Endothelial Cell ◽  
Coronary Artery ◽  
Mrna Sequencing ◽  
Markers Of Inflammation ◽  
Artery Disease

scholarly journals Blocking DNA Damage Repair May Be Involved in Stattic (STAT3 Inhibitor)-Induced FLT3-ITD AML Cell Apoptosis

2021 ◽  
Vol 9 ◽  
Author(s):  
Yuxuan Luo ◽  
Ying Lu ◽  
Bing Long ◽  
Yansi Lin ◽  
Yanling Yang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Myeloid Leukemia ◽  
Tandem Duplication ◽  
Dna Damage Repair ◽  
Underlying Mechanism ◽  
Internal Tandem Duplication ◽  
Mrna Sequencing ◽  
Damage Repair ◽  
Flt3 Inhibitors ◽  
Flt3 Internal Tandem Duplication

The FMS-like tyrosine kinase 3 (FLT3)- internal tandem duplication (ITD) mutation can be found in approximately 25% of all acute myeloid leukemia (AML) cases and is associated with a poor prognosis. The main treatment for FLT3-ITD-positive AML patients includes genotoxic therapy and FLT3 inhibitors, which are rarely curative. Inhibiting STAT3 activity can improve the sensitivity of solid tumor cells to radiotherapy and chemotherapy. This study aimed to explore whether Stattic (a STAT3 inhibitor) affects FLT3-ITD AML cells and the underlying mechanism. Stattic can inhibit the proliferation, promote apoptosis, arrest cell cycle at G0/G1, and suppress DNA damage repair in MV4-11cells. During the process, through mRNA sequencing, we found that DNA damage repair-related mRNA are also altered during the process. In summary, the mechanism by which Stattic induces apoptosis in MV4-11cells may involve blocking DNA damage repair machineries.

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