HIGHLY EFFICIENT IN VITRO SHOOT REGENERATION SYSTEM IN TOMATO AND EGG PLANT VIA SEEDLING DECAPITATION METHOD (SDM)

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

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In Vitro Shoot Regeneration of Georgia Plume, Elliottia racemosa, from Multiple Genotypes Collected from Wild Populations

HortScience ◽

10.21273/hortsci.46.2.287 ◽

2011 ◽

Vol 46 (2) ◽

pp. 287-290

Author(s):

Carrie A. Radcliffe ◽

James M. Affolter ◽

Hazel Y. Wetzstein

Keyword(s):

Tissue Culture ◽

Shoot Regeneration ◽

Adventitious Shoots ◽

Shoot Elongation ◽

Shoot Cultures ◽

Regeneration System ◽

Low Genetic Diversity ◽

Wide Range ◽

In Vitro Shoot Regeneration

Georgia plume (Elliottia racemosa, Ericaceae) is a threatened, woody plant endemic to Georgia's Coastal Plain region in the southeastern United States. Populations of the plant have a fragmented distribution within a restricted range and are characterized by low genetic diversity and a lack of sexual recruitment. Georgia plume cannot be effectively propagated using conventional methods. We have developed an in vitro shoot regeneration system that is effective with explants obtained from mature plants in the wild. The objective of this study was to determine the efficacy of using this in vitro protocol to regenerate proliferating shoot cultures from 34 georgia plume genotypes obtained from divergent populations. Young expanding leaves were cultured on Gamborg's media supplemented with 10 μM thidiazuron and 5 μM indole-3-acetic acid. After 8 weeks, tissues were transferred to a shoot elongation medium with 25 μM 2-isopentenyl adenine. Of the 34 genotypes tested, 91% formed shoot primordia and 85% regenerated shoots within 6 months of inoculation. This study verifies that tissue culture can be used to produce adventitious shoots from a wide range of georgia plume genotypes. Within a coordinated conservation program, tissue culture is a feasible system to use for safeguarding and reintroduction of genetically diverse plant material, which may be critical to the survival of this rare species.

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In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽

10.21273/hortsci.33.3.460e ◽

1998 ◽

Vol 33 (3) ◽

pp. 460e-460 ◽

Cited By ~ 1

Author(s):

Marisa F. de Oliveira ◽

Gerson R. de L. Fortes ◽

João B. da Silva

Keyword(s):

Shoot Regeneration ◽

Dichlorophenoxyacetic Acid ◽

Apple Rootstock ◽

Under Five ◽

Significant Difference ◽

Previously Treated ◽

2,4 Dichlorophenoxyacetic Acid ◽

In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

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Establishment of an efficient shoot regeneration system in vitro in Brassica rapa

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-021-10175-3 ◽

2021 ◽

Author(s):

Ying Zhao ◽

Shengnan Huang ◽

Yun Zhang ◽

Fengyan Shi ◽

Xuyao Liu ◽

...

Keyword(s):

Brassica Rapa ◽

Shoot Regeneration ◽

Regeneration System

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An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v18i1.3246 ◽

2009 ◽

Vol 18 (1) ◽

pp. 17-24

Author(s):

Md. Nesawar Miah ◽

Shahina Islam ◽

Syed Hadiuzzaman

Keyword(s):

Shoot Regeneration ◽

Multiple Shoots ◽

Multiple Shoot ◽

Nodal Explants ◽

Cow Dung ◽

Half Strength ◽

Multiple Shoot Regeneration ◽

Shoot Tip Explants ◽

In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

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