scholarly journals In Vitro Shoot Regeneration of Georgia Plume, Elliottia racemosa, from Multiple Genotypes Collected from Wild Populations

HortScience ◽  
2011 ◽  
Vol 46 (2) ◽  
pp. 287-290
Author(s):  
Carrie A. Radcliffe ◽  
James M. Affolter ◽  
Hazel Y. Wetzstein
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Shoot Elongation ◽  
Shoot Cultures ◽  
Regeneration System ◽  
Low Genetic Diversity ◽  
Wide Range ◽  
In Vitro Shoot Regeneration

Georgia plume (Elliottia racemosa, Ericaceae) is a threatened, woody plant endemic to Georgia's Coastal Plain region in the southeastern United States. Populations of the plant have a fragmented distribution within a restricted range and are characterized by low genetic diversity and a lack of sexual recruitment. Georgia plume cannot be effectively propagated using conventional methods. We have developed an in vitro shoot regeneration system that is effective with explants obtained from mature plants in the wild. The objective of this study was to determine the efficacy of using this in vitro protocol to regenerate proliferating shoot cultures from 34 georgia plume genotypes obtained from divergent populations. Young expanding leaves were cultured on Gamborg's media supplemented with 10 μM thidiazuron and 5 μM indole-3-acetic acid. After 8 weeks, tissues were transferred to a shoot elongation medium with 25 μM 2-isopentenyl adenine. Of the 34 genotypes tested, 91% formed shoot primordia and 85% regenerated shoots within 6 months of inoculation. This study verifies that tissue culture can be used to produce adventitious shoots from a wide range of georgia plume genotypes. Within a coordinated conservation program, tissue culture is a feasible system to use for safeguarding and reintroduction of genetically diverse plant material, which may be critical to the survival of this rare species.

scholarly journals In vitro shoot regeneration of boldo from leaf explants

2010 ◽  
Vol 40 (10) ◽  
pp. 2210-2213
Author(s):  
Monalize Salete Mota ◽  
Juliana de Magalhães Bandeira ◽  
Eugenia Jacira Bolacel Braga ◽  
Valmor João Bianchi ◽  
José Antonio Peters
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Development ◽  
High Potential ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Bud Induction ◽  
Molecular Studies ◽  
In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

scholarly journals SHOOT REGENERATION FROM LEAF TISSUES OF AMERICAN ELM AND SIBERIAN ELM.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 584f-584 ◽  
Author(s):  
Z. M. Cheng ◽  
N. O. Shi ◽  
L. Tokach ◽  
B. K. Gaschk
Keyword(s):  
Factorial Design ◽  
Shoot Regeneration ◽  
Regeneration System ◽  
Ulmus Americana ◽  
Regeneration Rate ◽  
American Elm ◽  
Wide Range ◽  
Leaf Tissues

Shoot regeneration was obtained from leaf tissues of American (Ulmus americana) and Siberian elm (U. pumila) seedlings germinated in vitro and in greenhouse. Murashige and Skoog (MS, 1962) media supplemented with 4 levels of BA (0, 5, 10, 15, 20 μM) and 3 levels of IBA (0, 2.5, 5.0 μM) were tested in a factorial design to find an optimal hormonal combination for shoot regeneration. Shoot regeneration was obtained from both species within 3-4 weeks in a wide range of media. The highest regeneration rate (50%) of American elm was in the medium containing 10 μM BA and 2.5 μM IBA. Incubation under the light was essential for a higher rate of regeneration. Gelrite was found as a better solidifying agent than agar. The progress is under way to achieve transgenic elms by combining this regeneration system with Agrobacterium-mediated transformation.

scholarly journals Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

2009 ◽  
Vol 21 (2) ◽  
pp. 43-52 ◽  
Author(s):  
Wojciech Litwińczuk ◽  
Ewa Okołotkiewicz ◽  
Iwona Matyaszek
Keyword(s):  
Gibberellic Acid ◽  
Adventitious Shoots ◽  
Shoot Culture ◽  
Shoot Elongation ◽  
Axillary Shoot ◽  
Shoot Cultures ◽  
Axillary Shoots ◽  
High Doses ◽  
Culture Growth

Abstract In this study, the influence of gibberellic acid (GA3) on strawberry in vitro shoot culture growth and development was investigated. ‘Senga Sengana’ and ‘Elsanta’ clones were grown on the medium recommended by Boxus (1999), supplemented with BA (0.5 mg dm-3), IBA (0.1 mg dm-3), glucose (40.0 g dm-3) and GA3 (0.1 - control, 1.0, 2.0, 5.0, 10.0 mg dm-3). In general, gibberellic acid improved axillary shoot elongation and reduced the growth of callus as well as the formation of roots and the development of adventitious shoots. GA3 applied at a concentration of 1.0-2.0 mg dm-3 significantly increased the number of axillary shoots (mainly crown shoots), whereas under higher (5.0-10.0 mg dm-3) doses it stimulated the development of runners. It seems that a new method of strawberry micropropagation based on the multiplication of axillary crowns and runners, which could also reduce the risk of domination of in vitro cultures with adventitious shoots, might be elaborated

In vitro shoot regeneration of mint (Mentha sp. L.) Using different types of explants and levels of benzylaminopurine

2018 ◽  
Vol 43 (4) ◽  
pp. 703-716
Author(s):  
KT Akter ◽  
MA Hoque
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Shoot Tip ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Different Types ◽  
Almost All ◽  
In Vitro Shoot Regeneration ◽  
Better Than

An experiment was conducted in the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706 from March 2013 to February 2014 for in vitro shoot regeneration of mint using different explants and levels of benzylaminopurine (BAP) in full strength MS media. Three different types of explants viz. nodal segment, shoot tip and leaf were evaluated using three levels of BAP (1.0, 2.0 and 3.0 mg/l) along with control for shoot regeneration. Results revealed that shoot tip and nodal segments performed better than leaf as explants in almost all the characters studied. Shoot tip and nodal segments initiated shoot within the shortest time of 9.6 and 10.6 days, respectively with 1.0 mg/l of BAP. Regarding number of shoot per explant and number of node/shoot, shoot tip and nodal segments along with 1.0 mg/l of BAP performed superior at almost all days after inoculation. In case of interaction of explants and BAP, better performance was recorded in most of the studied parameters from shoot tip and nodal segments alongwith 1.0 mg/l BAP. Therefore, for in vitro shoot regeneration of mint, shoot tip or nodal segment may be used with 1.0 mg/l of BAP.Bangladesh J. Agril. Res. 43(4): 703-716, December 2018

scholarly journals Effect of growth regulators on micropropagation of Rauvolfia serpentina (L.) Benth

2014 ◽  
Vol 6 (2) ◽  
pp. 507-511 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Large Scale ◽  
Shoot Elongation ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Axillary Shoots ◽  
Indole Butyric Acid ◽  
Large Scale Cultivation

An efficient protocol for micropropagation through in vitro culture of Rauvolfia serpentina was standardized. Out of different combination of phytohormone tested, MS media supplemented with 0.5 mg L-1Indole Acetic Acid + 0.5 mg L Nephthalene acetic acid was found to be finest for mean callus induction (62.66%) as well as callus mediated shoot regeneration with mean percentage response (56) and number of shoot per culture (5). In direct shoot regeneration, best growth of axillary shoots was obtained on MS media supplemented with 0.5 mg L-1 Indole Acetic Acid + 0.5 mg L-1 Benzyl Amino Purine with maximum mean percentage response(77.33) and number of shoots per culture (9.0) ,however the best shoot elongation of shoot was found on MS media supplemented with 3.0 mg L-1 IAA plus 3.0 mg L-1BAP with 6.50(mean) . Higher induction of root (88%) with mean number of root per culture (12) was observed in MS medium supplemented with Indole Butyric Acid (3.0 mg L-1). The rooted plantlets were successfully established in the field. The protocol was optimized by manipulations of different PGRs for enhanced multiplication. Protocol explained in this research paper provides a rapid plant regeneration system which could be used for production of large number of true to the type, uniform, disease free, elite, plantlets right through the year, which will make things easier for large scale cultivation of this endangered important medicinal plant.

scholarly journals Development of Insect-tolerant Plants with Somaclonal Variation

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 695c-695
Author(s):  
Mark Bridgen
Keyword(s):  
Somaclonal Variation ◽  
Adventitious Shoots ◽  
Trialeurodes Vaporariorum ◽  
Shoot Cultures ◽  
Plant Tolerance ◽  
Torenia Fournieri ◽  
Greenhouse Whitefly ◽  
Wide Range ◽  
Regenerated Plantlets

The potential value of somaclonal variation for economically important plants is well-documented. The process of somaclonal variation can arise from a controlled or a random source of variation. Variability can be obtained by applying cellular pressures and selection. Valuable resistance to diseases and nematodes has already been accomplished with somaclonal variation; now, plant tolerance to pests has been realized. Tetranychus urticae, the two-spotted spidermite, and Trialeurodes vaporariorum, the greenhouse whitefly, were disinfected and introduced to aseptic shoot cultures of Torenia fournieri. These pests were allowed to feed until such time that their populations decreased due to the absence of food. The plant cells that remained after feeding were induced to form adventitious shoots and plantlets. These regenerated plantlets were acclimated to greenhouse conditions and evaluated for tolerance to the pest to which they were subjected in vitro. Highly significant differences were found in somaclones for both the two-spotted spidermite and greenhouse whitefly when compared to control plants. A wide range of variability was observed among the somaclonal population. There were significantly fewer mite eggs laid on plants regenerated from in vitro cultures screened with two-spotted spidermites than on seed-sown controls. Regenerants from cultures screened with whiteflies in vitro had fewer eggs, immatures and live adults than controls. The potential for somaclonal variation to be used as a method to develop insect resistant plants will be discussed.

scholarly journals Adventitious Shoot Regeneration from In Vitro Leaves of Formosan Sweetgum (Liquidambar formosana L.)

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 721-723 ◽  
Author(s):  
L. Xu ◽  
G.F. Liu ◽  
M.Z. Bao
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
High Regeneration

Plantlets were regenerated from in vitro-grown leaf explants of five genotypes of Liquidambar formosana on WPM basal medium supplemented with different concentrations of TDZ and NAA. With the addition of 0.27 μm NAA, regeneration efficiency was increased by 2- to 4-fold over that with TDZ alone. Lower concentrations of TDZ (0.45–2.27 μm) were beneficial for regenerating shoot clusters. Four genotypes (P2, P6, P9, and P11) showed high regeneration rates (up to 90%), whereas genotype P13 showed a low capability for shoot regeneration on all media tested (<35%). For all five genotypes, the optimum medium for inducing adventitious shoots was WPM supplemented with 1.14 μm TDZ and 0.27 μm NAA, on which regeneration rate ranged from 72.6% to 89.5% and adventitious shoot clusters per regenerating leaf explant ranged from 2.63 to 3.11 in four genotypes (P2, P6, P9, and P11), while for P13, the regeneration rate and number of shoot clusters per regenerating explant were 23% and 1.39, respectively. Transfer of shoot clusters to WPM basal medium containing 0.54 μm NAA, 2.22 μm BA, and 1.44 μm GA3, resulted in shoot elongation. All the elongated shoots were rooted on WPM supplemented with 9.84 μm IBA, and plantlets were transplanted to soil successfully. Chemical names used: 6-benzyladenine (BA), gibberellic acid (GA3), indole-3-butyric acid (IBA), 1-naphthalene acetic acid (NAA), plant growth regulator (PGR), thidiazuron (TDZ), woody plant medium (WPM).

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

scholarly journals Rejuvenation of chicory and lettuce plants following phase change in tissue culture

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Anthony J. Conner ◽  
Helen Searle ◽  
Jeanne M. E. Jacobs
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Seed Set ◽  
Genetic Manipulation ◽  
Adventitious Shoot ◽  
In Vitro Flowering ◽  
Phase Changes ◽  
Adventitious Shoot Regeneration ◽  
Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

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