IC-PCR AMPLIFICATION OF APPLE STEM GROOVING VIRUS ISOLATES AND COMPARISON OF POLYMERASE AND COAT PROTEIN GENE SEQUENCES

1998 ◽  
pp. 113-118 ◽  
Author(s):  
S.J. Crossley ◽  
V. Jacobi ◽  
A.N. Adams
2001 ◽  
Vol 26 (3) ◽  
pp. 655-659 ◽  
Author(s):  
OSMAR NICKEL ◽  
THOR V.M. FAJARDO ◽  
WILHELM JELKMANN ◽  
GILMAR B. KUHN

Apple stem grooving virus (ASGV) is one of the most important viruses infecting fruit trees. This study aimed at the molecular characterization of ASGV infecting apple (Malus domestica) plants in Santa Catarina (SC). RNA extracted from plants infected with isolate UV01 was used as a template for RT-PCR using specific primers. An amplified DNA fragment of 755 bp was sequenced. The coat protein gene of ASGV isolate UV01 contains 714 nucleotides, coding for a protein of 237 amino acids with a predicted Mr of approximately 27 kDa. The nucleotide and the deduced amino acid sequences of the coat protein gene showed identities of 90.9% and 97.9%, respectively, with a Japanese isolate of ASGV. Very high amino acid homologies (98.7%) were also found with Citrus tatter leaf capillovirus (CTLV), a very close relative of ASGV. These results indicate low coat protein gene variability among Capillovirus isolates from distinct regions. In a restricted survey, mother stocks in orchards and plants introduced into the country for large scale fruit production were indexed and shown to be infected by ASGV (20%), usually in a complex with other (latent) apple viruses (80%).


2009 ◽  
Vol 19 (1) ◽  
pp. 91-94 ◽  
Author(s):  
Anuradha Negi ◽  
Tanuja Rana ◽  
Yogesh Kumar ◽  
Raja Ram ◽  
Vipin Hallan ◽  
...  

2011 ◽  
Vol 36 (2) ◽  
pp. 121-124 ◽  
Author(s):  
José Evando A. Beserra Jr. ◽  
Eduardo C. Andrade ◽  
Rosa F.R. Araújo Camarço ◽  
Aline K.Q. Nascimento ◽  
José Albérsio A. Lima

1996 ◽  
Vol 141 (12) ◽  
pp. 2277-2287 ◽  
Author(s):  
K. Kobayashi ◽  
P. Rabinowicz ◽  
F. Bravo-Almonacid ◽  
M. Helguera ◽  
V. Conci ◽  
...  

VirusDisease ◽  
2017 ◽  
Vol 28 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Atul B. Patil ◽  
Vijayendra S. Dalvi ◽  
Akhilesh A. Mishra ◽  
Bal Krishna ◽  
Abdul Azeez

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