RESISTANCE TO PASSION FRUIT WOODINESS VIRUS IN TRANSGENIC PLANTS OF THE YELLOW PASSION FRUIT EXPRESSING THE VIRAL COAT PROTEIN GENE

2007 ◽  
pp. 495-499 ◽  
Author(s):  
F. Trevisan ◽  
B.M.J. Mendes ◽  
S.C. Maciel ◽  
J.A.M. Rezende ◽  
M.L.C. Vieira ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Passion Fruit ◽  
Viral Coat Protein ◽  
Viral Coat ◽  
Passion Fruit Woodiness Virus ◽  
Yellow Passion Fruit

Peanut Stripe Potyvirus Resistance in Peanut (Arachis Hypogaea L.) Plants Carrying Viral Coat Protein Gene Sequences

2004 ◽  
Vol 13 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Colleen M. Higgins ◽  
Rhonda M. Hall ◽  
Neena Mitter ◽  
Alan Cruickshank ◽  
Ralf G. Dietzgen
Keyword(s):  
Coat Protein ◽  
Arachis Hypogaea ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Gene Sequences ◽  
Viral Coat Protein ◽  
Arachis Hypogaea L ◽  
Viral Coat

scholarly journals Resistance to Passion fruit woodiness virus in Transgenic Passionflower Expressing the Virus Coat Protein Gene

Plant Disease ◽  
2006 ◽  
Vol 90 (8) ◽  
pp. 1026-1030 ◽  
Author(s):  
F. Trevisan ◽  
B. M. J. Mendes ◽  
S. C. Maciel ◽  
M. L. C. Vieira ◽  
L. M. M. Meletti ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
In Vitro Selection ◽  
Passion Fruit ◽  
Cp Gene ◽  
Virus Coat ◽  
Passion Fruit Woodiness Virus ◽  
Leaf Disks ◽  
Yellow Passion Fruit

We report the use of the coat protein (CP) gene from Passion fruit woodiness virus (PWV) to produce resistant transgenic plants of yellow passion fruit. A full-length CP gene from a severe PWV isolate from the state of São Paulo, Brazil (PWV-SP) was cloned into pCAMBIA 2300 binary vector, which was further introduced into Agrobacterium tumefaciens strain EHA 105. Leaf disks were used as explants for transformation assays, e.g., 2,700 and 2,730 disks excised from plants from the Brazilian cultivars IAC-275 and IAC-277, respectively. In vitro selection was performed in kanamycin. After transferring to the elongation medium, 119 and 109 plantlets of IAC-275 and IAC-277, respectively, were recovered. Integration of the PWV CP gene was confirmed in seven of eight plants evaluated by Southern blot analysis, showing different numbers of insertional events for the CP gene. Three transgenic plants (T3, T4, and T7) expressed the expected transcript, but the 32 kDa PWV CP was detected by Western blot in only two plants (T3 and T4). The results of three successive mechanical inoculations against the transgenic plants using three PWV isolates showed that the primary transformant T2 of IAC-277 was immune to all isolates.

Resistance to wheat streak mosaic virus in transgenic wheat engineered with the viral coat protein gene

2002 ◽  
Vol 11 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Elumalai Sivamani ◽  
Christopher W. Brey ◽  
Luther E. Talbert ◽  
Mark A. Young ◽  
William E. Dyer ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Transgenic Wheat ◽  
Wheat Streak Mosaic Virus ◽  
Viral Coat Protein ◽  
Viral Coat

scholarly journals Restoration of Wild-Type Virus by Double Recombination of Tombusvirus Mutants with a Host Transgene

1999 ◽  
Vol 12 (2) ◽  
pp. 153-162 ◽  
Author(s):  
Marise Borja ◽  
Teresa Rubio ◽  
Herman B. Scholthof ◽  
Andrew O. Jackson
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Wild Type Virus ◽  
Type Virus ◽  
Virus Infections ◽  
Wild Type ◽  
Transgenic Lines ◽  
Double Recombination

Nicotiana benthamiana plants transformed with the coat protein gene of tomato bushy stunt virus (TBSV) failed to elicit effective virus resistance when inoculated with wild-type virus. Subsequently, R1 and R2 progeny from 13 transgenic lines were inoculated with a TBSV mutant containing a defective coat protein gene. Mild symptoms typical of those elicited in nontransformed plants infected with the TBSV mutant initially appeared. However, within 2 to 4 weeks, up to 20% of the transgenic plants sporadically began to develop the lethal syndrome characteristic of wild-type virus infections. RNA hybridization and immunoblot analyses of these plants and nontransformed N. benthamiana inoculated with virus from the transgenic lines indicated that wild-type virus had been regenerated by a double recombination event between the defective virus and the coat protein transgene. Similar results were obtained with a TBSV deletion mutant containing a nucleotide sequence marker, and with a chimeric cucumber necrosis virus (CNV) containing the defective TBSV coat protein gene. In both cases, purified virions contained wild-type TBSV RNA or CNV chimeric RNA derived by recombination with the transgenic coat protein mRNA. These results thus demonstrate that recombinant tombusviruses can arise frequently from viral genes expressed in transgenic plants.

IDENTIFICATION OF THE COAT PROTEIN GENE OF ARABIS MOSAIC NEPOVIRUS AND ITS EXPRESSION IN TRANSGENIC PLANTS

1992 ◽  
pp. 37-42 ◽  
Author(s):  
H. Steinkellner ◽  
A. Weinhäusl ◽  
M. Laimer ◽  
A. Machado ◽  
H. Katinger
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene
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