IN VITRO REGENERATION OF RHODODENDRONS VIA AXILLARY SHOOT AND SOMATIC EMBRYO INDUCTION

2010 ◽  
pp. 417-423
Author(s):  
H. Vejsadová
Keyword(s):  
Somatic Embryo ◽  
In Vitro Regeneration ◽  
Axillary Shoot ◽  
Embryo Induction ◽  
Somatic Embryo Induction

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

2010 ◽  
Vol 140 (2) ◽  
pp. 225-243 ◽  
Author(s):  
Priyanka Saha ◽  
Subhendu Bandyopadhyay ◽  
Sarmistha Sen Raychaudhuri
Keyword(s):  
Somatic Embryo ◽  
Nutrient Medium ◽  
Plantago Ovata ◽  
Embryo Induction ◽  
Somatic Embryo Induction

scholarly journals Induksi dan Proliferasi Embriogenesis Somatik In Vitro pada Lima Genotipe Kedelai

2017 ◽  
Vol 44 (3) ◽  
pp. 261
Author(s):  
Adam Saepudin ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Dan Sintho Wahyuning Ardie
Keyword(s):  
Somatic Embryo ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Induction Medium ◽  
Embryo Induction ◽  
Soybean Genotypes ◽  
Induction Experiment ◽  
Embryogenic Callus Induction ◽  
Somatic Embryo Induction

ABSTRACT<br /><br />Somatic embryo induction medium was reported to be genotype dependent for soybean. This study was aimed to obtain the optimum medium for embryo somatic induction and proliferation, and to regenerate somatic embryo of five soybean genotypes. Five soybean genotypes (Tanggamus, Anjasmoro, Yellow Biloxi, CG-22-10, and SP-10-4) were used in this study. The research was divided into four steps: (1) embryogenic callus induction of  five soybean genotypes, (2) embryogenic callus proliferation of five soybean genotypes, (3) optimation of embryo somatic induction on five soybean genotypes and (4) embryo somatic regeneration of five soybean genotypes. The induction experiment showed that based on number of embryogenic callus, the best somatic embryo-induction medium was 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5. Embryogenic callus number for each genotype tested was increased on proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5, and Yellow Biloxi gave the highest number of proliferated somatic embryos compared to other genotypes. Increasing number of globular somatic embryo of all genotypes was obtained from the optimation of somatic embryo induction media being used, and Tanggamus genotype gave the highest number of globular somatic embryo which followed by Yellow Biloxi genotype. Tanggamus and Yellow Biloxi genotypes were also successfully formed the four steps of somatic embryos (globular, heart, torpedo, and cotyledonary stages), but in regeneration medium of MS0 and media MS + sukrosa 10 g L-1 + GA3 2 mg L-1 + BAP 4 mg L-1 + Vit B5 only Tanggamus genotype was regenerated into plantlet.  <br /><br />Keywords: 2,4-D, NAA, somatic embryos, induction, proliferation<br /><br />

scholarly journals An efficient protocol for somatic embryogenesis of garlic (Allium sativum L.) using root tip as explant

2014 ◽  
Vol 12 (1) ◽  
pp. 1-6 ◽  
Author(s):  
MN Hassan ◽  
MS Haque ◽  
MM Hassan ◽  
MS Haque
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Callus Induction ◽  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Root Tip ◽  
Root Tips ◽  
Embryo Induction ◽  
Somatic Embryo Induction

Genetic improvement of garlic through conventional breeding is very difficult due to sterile nature of its flower. Hence, an alternative system is desirable to induce genetic variation. Tissue culture could be a good opportunities and somatic embryogenesis is one of the potential techniques of tissue culture for in vitro regeneration of garlic plant. The successes and production of somatic embryo depends on several factors such as optimization of media components, genotypes and explant type. Therefore, in the present investigation, garlic root tips were used as explant for callus and somatic embryo induction under different plant growth regulator combination. It was found that MS+1.0 mg l-1 2,4-D was the most favorable (86.10% regeneration with 2.19 cm callus diameter) for callus induction. This concentration also induced and produced good quality somatic embryo. In addition, MS+2.0 mg l-1 Kinetin gave better regeneration of somatic embryo and yielded the highest number (4.670) and longest length (7.0 cm) of shoots per callus. The procedure used a single hormonal signal for callus and somatic embryo induction as well as hormone free medium for further development of plantlet. Besides, maximum duration for callus induction and somatic embryo production was 17 and 10.67 days respectively. Thus, it appears that the protocol is cheap and time bound and particularly useful for conducting experiment for genetic improvement of garlic. Furthermore, as the protocol is cost effective, it can be further tested for commercial feasibility. DOI: http://dx.doi.org/10.3329/jbau.v12i1.20747 J. Bangladesh Agril. Univ. 12(1): 1-6, June 2014

Species-dependent divergent responses to in vitro somatic embryo induction in Passiflora spp.

2014 ◽  
Vol 120 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Yara Brito Chaim Jardim Rosa ◽  
Carolina Cassano Monte Bello ◽  
Marcelo Carnier Dornelas
Keyword(s):  
Somatic Embryo ◽  
Embryo Induction ◽  
Somatic Embryo Induction

scholarly journals Improvement of a Genetic Transformation System and Preliminary Study on the Function of LpABCB21 and LpPILS7 Based on Somatic Embryogenesis in Lilium pumilum DC. Fisch

2020 ◽  
Vol 21 (18) ◽  
pp. 6784
Author(s):  
Shengli Song ◽  
Rui Yan ◽  
Chunxia Wang ◽  
Jinxia Wang ◽  
Hongmei Sun
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Transformation ◽  
Somatic Embryo ◽  
Asymmetric Distribution ◽  
Embryo Induction ◽  
Transgenic Lines ◽  
Preliminary Study ◽  
Genetic Transformation System ◽  
Mutant Lines ◽  
Somatic Embryo Induction

Auxin transport mediates the asymmetric distribution of auxin that determines the fate of cell development. Agrobacterium-mediated genetic transformation is an important technical means to study gene function. Our previous study showed that the expression levels of LpABCB21 and LpPILS7 are significantly up-regulated in the somatic embryogenesis (SE) of Lilium pumilum DC. Fisch. (L. pumilum), but the functions of both genes remain unclear. Here, the genetic transformation technology previously developed by our team based on the L.pumilum system was improved, and the genetic transformation efficiency increased by 5.7–13.0%. Use of overexpression and CRISPR/Cas9 technology produced three overexpression and seven mutant lines of LpABCB21, and seven overexpression and six mutant lines of LpPILS7. Analysis of the differences in somatic embryo induction of transgenic lines confirmed that LpABCB21 regulates the early formation of the somatic embryo; however, excessive expression level of LpABCB21 inhibits somatic embryo induction efficiency. LpPILS7 mainly regulates somatic embryo induction efficiency. This study provides a more efficient method of genetic transformation of L. pumilum. LpABCB21 and LpPILS7 are confirmed to have important regulatory roles in L. pumilum SE thus laying the foundation for subsequent studies of the molecular mechanism of Lilium SE.

scholarly journals Micropropagation of Vanasushava pedata - An Endangered Medicinal Plant of South India

1970 ◽  
Vol 16 (2) ◽  
pp. 85-94 ◽  
Author(s):  
S Karuppusamy ◽  
C Kiranmai ◽  
V Aruna ◽  
T Pullaiah
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Continuous Production ◽  
Natural Habitat ◽  
Nodal Segments ◽  
Garden Soil ◽  
Axillary Shoot ◽  
Similar Frequency ◽  
Endangered Medicinal Plant

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

scholarly journals Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 398-402 ◽  
Author(s):  
Mohammed Elsayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Yougasphree Naidoo
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Naphthalene Acetic Acid ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Protocol ◽  
Regenerated Plantlets

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

scholarly journals In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan

2017 ◽  
Vol 4 (1) ◽  
pp. 21 ◽  
Author(s):  
Suman Parihar
Keyword(s):  
In Vitro Regeneration ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Adenine Sulphate ◽  
Bud Induction ◽  
Threatened Plant Species ◽  
Morphogenic Response ◽  
House Condition ◽  
Ceropegia Bulbosa

In vitro regeneration protocol has been standardized for highly medicinal and threatened succulent Ceropegia bulbosa Roxb. The paper focuses on morphogenic response of nodal explant when cultured on MS media. Murashige and Skoog medium supplemented with 6-benzyladenine (BA) (2.0 mgl-1) was found optimum for axillary shoot bud induction with 83.4 % response. Further shoots were multiplied through repetitive (3-4 times) transfer of the original explant and by subculture of the in vitro generated shoots. Maximum number of shoots 5.7±0.78 with shoot length of 3.6±0.82 cm was achieved on MS medium augmented with combination of 0.25 mgl-1 BA + 0.25 mgl-1 KN + 0.1 mgl-1 IAA and additives (50.0 mgl-1 ascorbic acid, 25 mgl-1 each of citric acid, arginine and adenine sulphate). For ex vitro rooting, pulse treatment of IBA 250 mgl-1 for 3 min was found optimum. The rooted shoots were successfully hardened in the green house condition (RH 75-80% at 26-28˚C) and about 80 % shoots were transferred to the garden.

Somatic embryo induction in Eucalyptus dunnii

1996 ◽  
Vol 45 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Regina R. Termignoni ◽  
Po-Jen Wang ◽  
Ching-Yeh Hu
Keyword(s):  
Somatic Embryo ◽  
Embryo Induction ◽  
Eucalyptus Dunnii ◽  
Somatic Embryo Induction
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