Somatic embryo induction in Eucalyptus dunnii

1996 ◽  
Vol 45 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Regina R. Termignoni ◽  
Po-Jen Wang ◽  
Ching-Yeh Hu
Keyword(s):  
Somatic Embryo ◽  
Embryo Induction ◽  
Eucalyptus Dunnii ◽  
Somatic Embryo Induction

scholarly journals Improvement of a Genetic Transformation System and Preliminary Study on the Function of LpABCB21 and LpPILS7 Based on Somatic Embryogenesis in Lilium pumilum DC. Fisch

2020 ◽  
Vol 21 (18) ◽  
pp. 6784
Author(s):  
Shengli Song ◽  
Rui Yan ◽  
Chunxia Wang ◽  
Jinxia Wang ◽  
Hongmei Sun
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Transformation ◽  
Somatic Embryo ◽  
Asymmetric Distribution ◽  
Embryo Induction ◽  
Transgenic Lines ◽  
Preliminary Study ◽  
Genetic Transformation System ◽  
Mutant Lines ◽  
Somatic Embryo Induction

Auxin transport mediates the asymmetric distribution of auxin that determines the fate of cell development. Agrobacterium-mediated genetic transformation is an important technical means to study gene function. Our previous study showed that the expression levels of LpABCB21 and LpPILS7 are significantly up-regulated in the somatic embryogenesis (SE) of Lilium pumilum DC. Fisch. (L. pumilum), but the functions of both genes remain unclear. Here, the genetic transformation technology previously developed by our team based on the L.pumilum system was improved, and the genetic transformation efficiency increased by 5.7–13.0%. Use of overexpression and CRISPR/Cas9 technology produced three overexpression and seven mutant lines of LpABCB21, and seven overexpression and six mutant lines of LpPILS7. Analysis of the differences in somatic embryo induction of transgenic lines confirmed that LpABCB21 regulates the early formation of the somatic embryo; however, excessive expression level of LpABCB21 inhibits somatic embryo induction efficiency. LpPILS7 mainly regulates somatic embryo induction efficiency. This study provides a more efficient method of genetic transformation of L. pumilum. LpABCB21 and LpPILS7 are confirmed to have important regulatory roles in L. pumilum SE thus laying the foundation for subsequent studies of the molecular mechanism of Lilium SE.

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

2010 ◽  
Vol 140 (2) ◽  
pp. 225-243 ◽  
Author(s):  
Priyanka Saha ◽  
Subhendu Bandyopadhyay ◽  
Sarmistha Sen Raychaudhuri
Keyword(s):  
Somatic Embryo ◽  
Nutrient Medium ◽  
Plantago Ovata ◽  
Embryo Induction ◽  
Somatic Embryo Induction

scholarly journals Induksi dan Proliferasi Embriogenesis Somatik In Vitro pada Lima Genotipe Kedelai

2017 ◽  
Vol 44 (3) ◽  
pp. 261
Author(s):  
Adam Saepudin ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Dan Sintho Wahyuning Ardie
Keyword(s):  
Somatic Embryo ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Induction Medium ◽  
Embryo Induction ◽  
Soybean Genotypes ◽  
Induction Experiment ◽  
Embryogenic Callus Induction ◽  
Somatic Embryo Induction

ABSTRACT<br /><br />Somatic embryo induction medium was reported to be genotype dependent for soybean. This study was aimed to obtain the optimum medium for embryo somatic induction and proliferation, and to regenerate somatic embryo of five soybean genotypes. Five soybean genotypes (Tanggamus, Anjasmoro, Yellow Biloxi, CG-22-10, and SP-10-4) were used in this study. The research was divided into four steps: (1) embryogenic callus induction of  five soybean genotypes, (2) embryogenic callus proliferation of five soybean genotypes, (3) optimation of embryo somatic induction on five soybean genotypes and (4) embryo somatic regeneration of five soybean genotypes. The induction experiment showed that based on number of embryogenic callus, the best somatic embryo-induction medium was 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5. Embryogenic callus number for each genotype tested was increased on proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5, and Yellow Biloxi gave the highest number of proliferated somatic embryos compared to other genotypes. Increasing number of globular somatic embryo of all genotypes was obtained from the optimation of somatic embryo induction media being used, and Tanggamus genotype gave the highest number of globular somatic embryo which followed by Yellow Biloxi genotype. Tanggamus and Yellow Biloxi genotypes were also successfully formed the four steps of somatic embryos (globular, heart, torpedo, and cotyledonary stages), but in regeneration medium of MS0 and media MS + sukrosa 10 g L-1 + GA3 2 mg L-1 + BAP 4 mg L-1 + Vit B5 only Tanggamus genotype was regenerated into plantlet.  <br /><br />Keywords: 2,4-D, NAA, somatic embryos, induction, proliferation<br /><br />

scholarly journals An efficient protocol for somatic embryogenesis of garlic (Allium sativum L.) using root tip as explant

2014 ◽  
Vol 12 (1) ◽  
pp. 1-6 ◽  
Author(s):  
MN Hassan ◽  
MS Haque ◽  
MM Hassan ◽  
MS Haque
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Callus Induction ◽  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Root Tip ◽  
Root Tips ◽  
Embryo Induction ◽  
Somatic Embryo Induction

Genetic improvement of garlic through conventional breeding is very difficult due to sterile nature of its flower. Hence, an alternative system is desirable to induce genetic variation. Tissue culture could be a good opportunities and somatic embryogenesis is one of the potential techniques of tissue culture for in vitro regeneration of garlic plant. The successes and production of somatic embryo depends on several factors such as optimization of media components, genotypes and explant type. Therefore, in the present investigation, garlic root tips were used as explant for callus and somatic embryo induction under different plant growth regulator combination. It was found that MS+1.0 mg l-1 2,4-D was the most favorable (86.10% regeneration with 2.19 cm callus diameter) for callus induction. This concentration also induced and produced good quality somatic embryo. In addition, MS+2.0 mg l-1 Kinetin gave better regeneration of somatic embryo and yielded the highest number (4.670) and longest length (7.0 cm) of shoots per callus. The procedure used a single hormonal signal for callus and somatic embryo induction as well as hormone free medium for further development of plantlet. Besides, maximum duration for callus induction and somatic embryo production was 17 and 10.67 days respectively. Thus, it appears that the protocol is cheap and time bound and particularly useful for conducting experiment for genetic improvement of garlic. Furthermore, as the protocol is cost effective, it can be further tested for commercial feasibility. DOI: http://dx.doi.org/10.3329/jbau.v12i1.20747 J. Bangladesh Agril. Univ. 12(1): 1-6, June 2014

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