scholarly journals In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan

2017 ◽  
Vol 4 (1) ◽  
pp. 21 ◽  
Author(s):  
Suman Parihar
Keyword(s):  
In Vitro Regeneration ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Adenine Sulphate ◽  
Bud Induction ◽  
Threatened Plant Species ◽  
Morphogenic Response ◽  
House Condition ◽  
Ceropegia Bulbosa

In vitro regeneration protocol has been standardized for highly medicinal and threatened succulent Ceropegia bulbosa Roxb. The paper focuses on morphogenic response of nodal explant when cultured on MS media. Murashige and Skoog medium supplemented with 6-benzyladenine (BA) (2.0 mgl-1) was found optimum for axillary shoot bud induction with 83.4 % response. Further shoots were multiplied through repetitive (3-4 times) transfer of the original explant and by subculture of the in vitro generated shoots. Maximum number of shoots 5.7±0.78 with shoot length of 3.6±0.82 cm was achieved on MS medium augmented with combination of 0.25 mgl-1 BA + 0.25 mgl-1 KN + 0.1 mgl-1 IAA and additives (50.0 mgl-1 ascorbic acid, 25 mgl-1 each of citric acid, arginine and adenine sulphate). For ex vitro rooting, pulse treatment of IBA 250 mgl-1 for 3 min was found optimum. The rooted shoots were successfully hardened in the green house condition (RH 75-80% at 26-28˚C) and about 80 % shoots were transferred to the garden.

scholarly journals IN VITRO REGENERATION OF LITCHI (Litchi chinensis SONN.) THROUGH SHOOT BUD CULTURE

2020 ◽  
pp. 141-146
Author(s):  
Safeer ud Din ◽  
◽  
Waqar Shafqat
Keyword(s):  
In Vitro Regeneration ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Shoot Bud ◽  
Full Strength ◽  
In Vitro Shoots ◽  
Survival Frequency ◽  
Shoot Bud Culture ◽  
Positive Effect

Attempts were made to develop protocol for in vitro regeneration of litchi through axillary shoot bud cultures. The problem of microbial contamination, phenolic exudation and media browning was controlled up to some extend by pretreatment and rapid subculturing. A high frequency (51 %) shoot induction and differentiation was obtained in litchi Gola variety axillary explants on MS medium containing GA3 and BAP (1 mg/l), Kin (2 mg/l). In vitro raised shoots better proliferated in medium containing 2 mg/l BAP. BAP had positive effect on multiplication and growth of shoots but higher concentration than 2 mg/l reduced growth. Maximum rooting frequency (66.67%) with healthier roots was obtained in shoots cultured on full strength MS medium supplemented with IBA (2 mg/l). Plants with well-developed roots were transferred to soil with survival frequency of 57%. A combination of BAP and GA3 (1 mg/l), KIN (2 mg/l) was effective in establishment of cultures. While BAP (2 mg/l) and KIN (3 mg/l) was good for better flourishing in vitro raised shoots. 6-benzylaminepurine had positive effect on multiplication and growth of in vitro shoots but concentration exceeding 2 mg/l decreased growth. Full strength MS medium containing 2 mg/l IBA under dark condition promoted rooting in in vitro raised shoots. The protocol established could prove advantageous to the horticulturists and the industry for developing trees true to the parental type.

scholarly journals In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

2016 ◽  
Vol 26 (2) ◽  
pp. 255-266 ◽  
Author(s):  
Pranati Nayak ◽  
Kalidass C
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Garden Soil ◽  
Induction Medium ◽  
Root Induction ◽  
Shoot Buds ◽  
Shoot Bud ◽  
Cotyledon Explants ◽  
Bud Induction

Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA3. The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.Plant Tissue Cult. & Biotech. 26(2): 255-266, 2016 (December)

scholarly journals Micropropagation of Vanasushava pedata - An Endangered Medicinal Plant of South India

1970 ◽  
Vol 16 (2) ◽  
pp. 85-94 ◽  
Author(s):  
S Karuppusamy ◽  
C Kiranmai ◽  
V Aruna ◽  
T Pullaiah
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Continuous Production ◽  
Natural Habitat ◽  
Nodal Segments ◽  
Garden Soil ◽  
Axillary Shoot ◽  
Similar Frequency ◽  
Endangered Medicinal Plant

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

scholarly journals Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

2015 ◽  
Vol 24 (2) ◽  
pp. 223-234
Author(s):  
Shruti Bardar ◽  
Varsha Khurana Kaul ◽  
Sumita Kachhwaha ◽  
SL Kothari
Keyword(s):  
Basal Medium ◽  
Genetic Fidelity ◽  
Ex Situ ◽  
Nutrient Level ◽  
Induction Medium ◽  
Eclipta Alba ◽  
Shoot Bud ◽  
Bud Induction ◽  
Regeneration Response

This study highlights the effect of different inorganic micronutrients like copper, cobalt, molybdenum, zinc, boron, iodine, iron and manganese in accelerating and amplifying in vitro shoot bud induction and proliferation of a medicinally important plant, Eclipta alba (L.) Hassk. Direct shoot bud induction was observed on MS fortified with Kn (2 mg/l). However, maximum number of shoots was achieved when GA3, 0.5 mg/l was added to induction medium along with 1?M copper sulphate (ten times the normal MS level). Optimization of nutrient level in the basal medium promoted maximum regeneration response from both shoot tips and nodal explants. Elongated shoots were rooted in MS supplemented with IBA, 1.0 mg/l. Healthy, green plantlets with well developed roots, flowered normally in the field. Genetic stability of micropropagated plantlets was evaluated using RAPD markers. The amplification products were monomorphic in micropropagated plantlets and similar to those of mother plant revealing the genetic uniformity of plantlets. The regeneration protocol is highly efficient and reproducible so would be useful for mass multiplication, ex situ conservation and genetic transformation of E. alba (L.) Hassk.Plant Tissue Cult. & Biotech. 24(2): 223-234, 2014 (December)

scholarly journals Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 398-402 ◽  
Author(s):  
Mohammed Elsayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Yougasphree Naidoo
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Naphthalene Acetic Acid ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Protocol ◽  
Regenerated Plantlets

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals High Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale - AB Genome

2011 ◽  
Vol 21 (2) ◽  
pp. 199-205 ◽  
Author(s):  
K. Girish Kumar ◽  
V. Krishna ◽  
Venkatesh Venkatesh ◽  
K. Pradeep
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Floral Part ◽  
Bud Formation ◽  
Shoot Bud ◽  
Immature Male ◽  
Floral Explants ◽  
High Frequency Induction

High frequency in vitro regeneration for mass multiplication from immature male floral explants of Musa paradisica cv. Puttabale on MS supplemented with adenine sulfate (160 mg/l), tyrosine (100 mg/l), sucrose (40 g/l) and gelled with 0.8 g/l agar was attempted.  For callus induction the combinations of 2, 4-D and BAP were tested at 1.0 - 10.0 mg/l and 0.5 - 5.0 mg/l, respectively. For shoot bud formation combinations of BAP and TDZ were also tested at 1.0 - 5.0 mg/l and 0.1 - 0.5 mg/l, respectively.  Luxuriant proliferation and high frequency induction (97.0%) of  callus  was  noticed from the  accessory  floral  part  of  the  explant  at 7.0 mg/l 2, 4-D and 1.0 mg/l BAP, later it preceded towards the gynoecium. Interaction of BAP (2.0 – 5.0 mg/l) and TDZ (0.2 - 0.5 mg/l) would provoke high frequency shoot bud differentiation from the floral calli and a mean of 29.40 ± 6.10 shootlets per callus was obtained at 4 mg/l BAP and 0.4 mg/l TDZ. Rooting of the microshoots was achieved on MS containing 0.6 mg/l NAA and 0.2% activated charcoal.     Key words: Musa pardisica, Puttabale, Regeneration, Male floral explants.   D. O. I. 10.3329/ptcb.v21i2.10243   Plant Tissue Cult. & Biotech. 21(2): 199-205, 2011 (December)

scholarly journals In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis

2016 ◽  
Vol 8 (2) ◽  
pp. 724-729
Author(s):  
Sukhjit Kaur
Keyword(s):  
In Vitro Regeneration ◽  
Growth Hormones ◽  
Garden Soil ◽  
Direct Regeneration ◽  
Ms Medium ◽  
Citrus Jambhiri ◽  
Rough Lemon ◽  
Induction Frequency ◽  
Bud Induction

The effect of Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones on direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied. The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants. The epicotyls segments with proliferated buds were transferred to elongation media in order to improve the recovery of normal shoots. Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1).These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0). Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1.The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1). The hardened plants were successfully established in the soil. The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance.

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