Molecular characterization of ilarvirus Prunus necrotic ring spot virus (PNRSV) and Prune dwarf virus (PDV) in Spanish autochthonous almond cultivars

2018 ◽  
pp. 353-356
Author(s):  
M. Rubio ◽  
P. Martínez-Gómez ◽  
V. Pallás ◽  
F. Dicenta ◽  
J.A. Sánchez-Navarro
Keyword(s):  
Molecular Characterization ◽  
Dwarf Virus ◽  
Spot Virus ◽  
Prune Dwarf Virus ◽  
Ring Spot

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

2003 ◽  
Vol 114 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Genet Mekuria ◽  
Sunita A. Ramesh ◽  
Evita Alberts ◽  
Terry Bertozzi ◽  
Michelle Wirthensohn ◽  
...  
Keyword(s):  
Prunus Dulcis ◽  
Dwarf Virus ◽  
Rt Pcr ◽  
Spot Virus ◽  
Prune Dwarf Virus ◽  
Ring Spot

Molecular characterization of Prune dwarf virus cherry isolates from Slovakia shows their substantial variability and reveals recombination events in PDV RNA3

2016 ◽  
Vol 147 (4) ◽  
pp. 877-885 ◽  
Author(s):  
Lukáš Predajňa ◽  
Nina Sihelská ◽  
Daniela Benediková ◽  
Katarína Šoltys ◽  
Thierry Candresse ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Dwarf Virus ◽  
Prune Dwarf Virus

scholarly journals Horizontal Spread of Ilarviruses in Young Trees of Several Peach Cultivars

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 75-77 ◽  
Author(s):  
J. K. Uyemoto ◽  
L. R. Bulluck ◽  
Sarah Pethybridge ◽  
Bruce McCorkell ◽  
W. K. Asai
Keyword(s):  
Prunus Persica ◽  
Dwarf Virus ◽  
Virus Infections ◽  
Spot Virus ◽  
Prune Dwarf Virus ◽  
Biological Assays ◽  
Growing Seasons ◽  
Horizontal Spread ◽  
Ring Spot ◽  
Peach Orchard

Natural spread of Prune dwarf virus (PDV) and Prunus necrotic ring-spot virus (PNRSV) was monitored among healthy trees of six peach (Prunus persica (L.) Batsch) cultivars interplanted into a mature peach orchard between virus-infected trees of the cultivar Carson. Spread of PDV to a few trees of cvs. Andross, Carson, and Halford occurred during bloom just after the dormant test trees were planted in the orchard, as indicated by serological and biological assays during the next season. In contrast, virus infections in cvs. Dr. Davis, Loadel, and Ross were not detected until the fourth to sixth growing seasons. By the seventh season, cumulative transmissions for PDV and PNRSV were 41 and 9, respectively, which included double virus infections in five test trees. Significant differences in cumulative transmission of PDV but not of PNRSV occurred by the fourth season (P = 0.0020). Overall, virus infections among the six cultivars ranged from 70 to 100%.

scholarly journals Incidence of Prune dwarf virus and Prunus necrotic ring spot virus in orchards of sweet and sour cherry in the Czech Republic: Short communication

2010 ◽  
Vol 37 (No. 3) ◽  
pp. 118-120 ◽  
Author(s):  
J. Suchá ◽  
L. Svobodová
Keyword(s):  
Czech Republic ◽  
Sour Cherry ◽  
Dwarf Virus ◽  
Infection Level ◽  
Spot Virus ◽  
The Czech Republic ◽  
Field Surveys ◽  
Prune Dwarf Virus ◽  
Ring Spot ◽  
Cherry Trees

During 2006–2008 field surveys were carried out in the important cherry growing areas of the Czech Republic to assess the incidence of Prune dwarf virus and Prunus necrotic ring spot virus in commercial orchards and nurseries. A total of 1,438 samples from 1,198 sweet cherry trees and from 240 sour cherry trees were tested by ELISA for the presence of Prune dwarf virus and Prunus necrotic ring spot virus. The overall average infection level was 17.7%. The most infected species were sour cherry trees (22.5%). The most frequently detected virus was Prune dwarf virus (10.9%). Prunus necrotic ring spot virus occurred in 6.3% of samples. Our study provided an indication of a sanitary status of sweet and sour cherry in commercial orchards and nurseries in the Czech Republic.  

scholarly journals Molecular characterization of barley yellow dwarf virus in Tunisia

2014 ◽  
Vol 58 (03) ◽  
pp. 214-222 ◽  
Author(s):  
M. BOUALLEGUE ◽  
M. MEZGHANI-KHEMAKHEM ◽  
D. BOUKTILA ◽  
H. MAKNI ◽  
M. MAKNI
Keyword(s):  
Molecular Characterization ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Genomic RNA accumulation of gentian ovary ring-spot virus and raspberry bushy dwarf virus in pollen tubes

2018 ◽  
Vol 84 (5) ◽  
pp. 376-380 ◽  
Author(s):  
Masamichi Isogai ◽  
Youhei Suzuki ◽  
Takanori Matsudaira ◽  
Nobuyuki Yoshikawa
Keyword(s):  
Pollen Tubes ◽  
Dwarf Virus ◽  
Genomic Rna ◽  
Spot Virus ◽  
Raspberry Bushy Dwarf Virus ◽  
Rna Accumulation ◽  
Ring Spot

Molecular characterization of the genome segments S4, S6 and S7 of rice gall dwarf virus

2007 ◽  
Vol 152 (9) ◽  
pp. 1593-1602 ◽  
Author(s):  
H. M. Zhang ◽  
J. Yang ◽  
X. Xin ◽  
J. P. Chen ◽  
M. J. Adams
Keyword(s):  
Molecular Characterization ◽  
Dwarf Virus ◽  
Rice Gall Dwarf Virus

scholarly journals Identification and molecular characterization of onion yellow dwarf virus (OYDV) in Allium cepa crops in Poland

2021 ◽  
Vol 61 (3) ◽  
pp. 214-220
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Coat Protein Sequence ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Rt Pcr ◽  
Pcr Products ◽  
Yellow Dwarf Virus

Onion yellow dwarf virus is distributed worldwide significantly reducing yield of crops from the Allium genus. The aim of the study was the detection and molecular characterization of newly identified OYDV isolates infecting onions in Poland. The virus was detected by transmission electron microscopy and RT-PCR techniques using two pairs of diagnostic primers: OYDV-NibCPF1/R1 and OYDV-CPF2/R2. The specificity of obtained RT-PCR products was confirmed by Sanger sequencing and received viral coat protein sequence was used for phylogenetic analysis. The phylogenetic analysis was carried out using CP sequences of the new Polish onion isolate obtained in this study and 37 other sequences of OYDV retrieved from GenBank. The analysis revealed that the Polish OYDV isolate is the most similar to the OYDV isolates derived from onions from Argentina and Germany, which may indicate their common origin. Moreover, it was observed that the Polish onion and garlic isolates are very diverse and belong to different phylogroups.

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