Genomic RNA accumulation of gentian ovary ring-spot virus and raspberry bushy dwarf virus in pollen tubes

2018 ◽  
Vol 84 (5) ◽  
pp. 376-380 ◽  
Author(s):  
Masamichi Isogai ◽  
Youhei Suzuki ◽  
Takanori Matsudaira ◽  
Nobuyuki Yoshikawa
Keyword(s):  
Pollen Tubes ◽  
Dwarf Virus ◽  
Genomic Rna ◽  
Spot Virus ◽  
Raspberry Bushy Dwarf Virus ◽  
Rna Accumulation ◽  
Ring Spot

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

2003 ◽  
Vol 114 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Genet Mekuria ◽  
Sunita A. Ramesh ◽  
Evita Alberts ◽  
Terry Bertozzi ◽  
Michelle Wirthensohn ◽  
...  
Keyword(s):  
Prunus Dulcis ◽  
Dwarf Virus ◽  
Rt Pcr ◽  
Spot Virus ◽  
Prune Dwarf Virus ◽  
Ring Spot

scholarly journals Pollen tubes introduce Raspberry bushy dwarf virus into embryo sacs during fertilization processes

Virology ◽  
2015 ◽  
Vol 484 ◽  
pp. 341-345 ◽  
Author(s):  
Masamichi Isogai ◽  
Tetu Yoshida ◽  
Takuya Shimura ◽  
Nobuyuki Yoshikawa
Keyword(s):  
Pollen Tubes ◽  
Dwarf Virus ◽  
Raspberry Bushy Dwarf Virus

scholarly journals Horizontal Spread of Ilarviruses in Young Trees of Several Peach Cultivars

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 75-77 ◽  
Author(s):  
J. K. Uyemoto ◽  
L. R. Bulluck ◽  
Sarah Pethybridge ◽  
Bruce McCorkell ◽  
W. K. Asai
Keyword(s):  
Prunus Persica ◽  
Dwarf Virus ◽  
Virus Infections ◽  
Spot Virus ◽  
Prune Dwarf Virus ◽  
Biological Assays ◽  
Growing Seasons ◽  
Horizontal Spread ◽  
Ring Spot ◽  
Peach Orchard

Natural spread of Prune dwarf virus (PDV) and Prunus necrotic ring-spot virus (PNRSV) was monitored among healthy trees of six peach (Prunus persica (L.) Batsch) cultivars interplanted into a mature peach orchard between virus-infected trees of the cultivar Carson. Spread of PDV to a few trees of cvs. Andross, Carson, and Halford occurred during bloom just after the dormant test trees were planted in the orchard, as indicated by serological and biological assays during the next season. In contrast, virus infections in cvs. Dr. Davis, Loadel, and Ross were not detected until the fourth to sixth growing seasons. By the seventh season, cumulative transmissions for PDV and PNRSV were 41 and 9, respectively, which included double virus infections in five test trees. Significant differences in cumulative transmission of PDV but not of PNRSV occurred by the fourth season (P = 0.0020). Overall, virus infections among the six cultivars ranged from 70 to 100%.

scholarly journals Horizontal pollen transmission of Gentian ovary ring-spot virus is initiated during penetration of the stigma and style by infected pollen tubes

Virology ◽  
2017 ◽  
Vol 503 ◽  
pp. 6-11 ◽  
Author(s):  
Masamichi Isogai ◽  
Yukie Kamata ◽  
Syunpei Ando ◽  
Misaki Kamata ◽  
Asuka Shirakawa ◽  
...  
Keyword(s):  
Pollen Tubes ◽  
Spot Virus ◽  
Pollen Transmission ◽  
Ring Spot

scholarly journals Incidence of Prune dwarf virus and Prunus necrotic ring spot virus in orchards of sweet and sour cherry in the Czech Republic: Short communication

2010 ◽  
Vol 37 (No. 3) ◽  
pp. 118-120 ◽  
Author(s):  
J. Suchá ◽  
L. Svobodová
Keyword(s):  
Czech Republic ◽  
Sour Cherry ◽  
Dwarf Virus ◽  
Infection Level ◽  
Spot Virus ◽  
The Czech Republic ◽  
Field Surveys ◽  
Prune Dwarf Virus ◽  
Ring Spot ◽  
Cherry Trees

During 2006–2008 field surveys were carried out in the important cherry growing areas of the Czech Republic to assess the incidence of Prune dwarf virus and Prunus necrotic ring spot virus in commercial orchards and nurseries. A total of 1,438 samples from 1,198 sweet cherry trees and from 240 sour cherry trees were tested by ELISA for the presence of Prune dwarf virus and Prunus necrotic ring spot virus. The overall average infection level was 17.7%. The most infected species were sour cherry trees (22.5%). The most frequently detected virus was Prune dwarf virus (10.9%). Prunus necrotic ring spot virus occurred in 6.3% of samples. Our study provided an indication of a sanitary status of sweet and sour cherry in commercial orchards and nurseries in the Czech Republic.  

Properties of the single protein and two nucleic acids of tomato ring-spot virus

1977 ◽  
Vol 55 (8) ◽  
pp. 1028-1037 ◽  
Author(s):  
Wayne R. Allen ◽  
H. F. Dias
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Gel Electrophoresis ◽  
Average Molecular Weight ◽  
Virus Genome ◽  
Protein Subunit ◽  
Spot Virus ◽  
Single Protein ◽  
Middle Component ◽  
Ring Spot

Purified preparations of several isolates of tomato ring-spot virus were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two individual nucleoprotein components. Acrylamide-gel electrophoresis showed that the lighter (middle) component contained a nucleic acid (RNA 2) that was distinct from the species (RNA 1) contained in the heavier (bottom) component. The bottom was more infectious than the middle component and infectivity was enhanced by mixing the components, indicating that the virus genome is divided between component types. Similar results were obtained from infectivity tests on the two nucleic acids. The nucleic acid contents of the middle and bottom components were about 40 and 41%, respectively. The average molecular weights of RNA 2 and RNA 1 from three virus isolates, as determined by acrylamide-gel electrophoresis, were 2.5 and 2.6 × 106, respectively. Molecular complexing between the RNA species during electrophoresis was prevented with the use of formamide. The single protein subunit from the same three isolates had an average molecular weight of about 58 000. Serological comparisons of five tomato ring-spot isolates associated with diseases of fruit trees and grapevines indicated that only the grape yellow vein strain was antigenically distinct. These and other properties indicate that this virus is similar to other members of the nepovirus group.

scholarly journals Dual Resistance of Melon to Fusarium oxysporum Races 0 and 2 and to Papaya ring-spot virus is Controlled by a Pair of Head-to-Head-Oriented NB-LRR Genes of Unusual Architecture

2013 ◽  
Vol 6 (1) ◽  
pp. 235-238 ◽  
Author(s):  
Yariv Brotman ◽  
Michael Normantovich ◽  
Zachi Goldenberg ◽  
Zvi Zvirin ◽  
Irina Kovalski ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Spot Virus ◽  
Papaya Ring Spot Virus ◽  
Ring Spot
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