Agrobacterium-mediated genetic transformation of 146-2 Russian apricot and plum rootstock with the gfp gene

2020 ◽  
pp. 95-100
Author(s):  
L.Y. Mourenets ◽  
A.S. Pushin ◽  
S.V. Dolgov
Keyword(s):  
Genetic Transformation ◽  
Gfp Gene

scholarly journals Agrobacterium tumefaciens-mediated Transformation of Embryogenic Callus and Somatic Embryos of the Banana cv “Ambon Lumut” (Musa acuminata)

2017 ◽  
Vol 2 (6) ◽  
pp. 599 ◽  
Author(s):  
Tifa R. Kusumastuti ◽  
Rizkita R. Esyantia ◽  
Fenny M. Dwivany
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Crop Improvement ◽  
Pcr Analysis ◽  
Abiotic Factor ◽  
Gfp Gene ◽  
Culture Transformation ◽  
Biotechnological Approach

Banana is one of the major fruit crops, though its conventional breeding has limitations, such as sterility and high polyploidy  levels.  Biotechnological  approach  using genetic  transformation  crop for improvement  offers  an alternative  solution.  In  this  study  a  protocol  was developed  for  establishing genetic  transformation  from embryogenic callus and somatic embryos of the banana cv Ambon Lumut . Embryogenic callus was obtained in ID4 medium (MS-based medium) supplemented with 1 mg L-1 IAA, 4 mg L-1 2,4D, and 0.03 g L-1 active charcoal. Embryogenic callus was transferred into liquid mediu m to establish somatic embryos. Embryogenic callus and somatic embryos were used for Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain A GL1, containing pART-TEST7 p lasmid with gfp gene as a reporter and CaM V35S as a promoter, was used for transformations. The embryogenic callus and somatic embryos were transformed using heat-shock method followed by centrifugation  (2000 rpm) and co-cult ivation in liquid medium containing acetosyringone (100 M) for 3 days. Results of the GFP analysis showed transient expression from gfp gene reporter in transformed embryogenic callus and somatic embryos. Transformation efficiency in somatic embryos (85,9%) was higher than  that in embryogenic callus (32.09%). PCR analysis using CaMV primer showed bands that compatible with CaMV35S promoter at 507 bp. This is a report showing establisment of embryogenic callus and somatic embryo culture transformation by using A. tumefaciens-mediated transformation protocol of the local banana cv Ambon Lumut. This study proved  the huge potential for genetic transformation of banana cv Ambon Lumut for crop improvement, such as pest or disease  resistance and abiotic factor stress tolerance. Keywords: banana; embryogenic callus; somatic embryos.

scholarly journals Genetic Transformation of ‘Kyoho’ Grape with a GFP Gene

2006 ◽  
Vol 75 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Ikuko Nakajima ◽  
Nagao Matsuta ◽  
Toshiya Yamamoto ◽  
Shingo Terakami ◽  
Junichi Soejima
Keyword(s):  
Genetic Transformation ◽  
Gfp Gene

scholarly journals Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

2012 ◽  
Vol 43 (3) ◽  
pp. 1222-1229 ◽  
Author(s):  
Cecilia Armesto ◽  
Fernanda Gonçalves Martins Maia ◽  
Mário Sobral de Abreu ◽  
Antonia dos Reis Figueira ◽  
Bruno Marques da Silva ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Colletotrichum Gloeosporioides ◽  
Gfp Gene

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba

Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation

2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Xiaomei Li ◽  
Liu Liu ◽  
Xiangyu Liu ◽  
Yan Hou ◽  
Bingyin Xu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Resistance Gene ◽  
Expression Vectors

Construction and Genetic Transformation of TomatoARF4RNA Interference Expression Vector with Fruit Specific Promoter

2012 ◽  
Vol 18 (2) ◽  
pp. 206
Author(s):  
Yuanyuan FENG ◽  
Yingnan LI ◽  
Pei HOU ◽  
Jingrong ZHANG ◽  
Songhu WANG ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Expression Vector

Genetic transformation of commercial breeding lines of alfalfa (Medicago sativa)

1995 ◽  
Vol 42 (2) ◽  
pp. 129-140 ◽  
Author(s):  
R�jean Desgagn�s ◽  
Serge Laberge ◽  
Guy Allard ◽  
Habib Khoudi ◽  
Yves Castonguay ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Medicago Sativa ◽  
Breeding Lines ◽  
Commercial Breeding
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