An efficient in vitro plantlet regeneration of an ornamental aquatic plant Anubias heterophylla through shoot tip culture

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1. Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication D. O. I. 10.3329/ptcb.v21i2.10241 Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

Download Full-text

Elimination of a new ampelovirus (GLRaV-Pr) and Grapevine rupestris stem pitting associated virus (GRSPaV) from two Vitis vinifera cultivars combining in vitro thermotherapy with shoot tip culture

Scientia Horticulturae ◽

10.1016/j.scienta.2009.08.016 ◽

2009 ◽

Vol 123 (2) ◽

pp. 280-282 ◽

Cited By ~ 25

Author(s):

V.I. Maliogka ◽

F.G. Skiada ◽

E.P. Eleftheriou ◽

N.I. Katis

Keyword(s):

Vitis Vinifera ◽

Shoot Tip ◽

Shoot Tip Culture ◽

Rupestris Stem Pitting ◽

Stem Pitting

Download Full-text

THE INFLUENCE OF HIGH TEMPERATURES AND BENZYLADENINE ON ROOT INDUCTION DURING IN VITRO SHOOT TIP CULTURE IN VITIS VINIFERA L.

Acta Horticulturae ◽

10.17660/actahortic.1988.227.99 ◽

1988 ◽

pp. 479-481 ◽

Cited By ~ 1

Author(s):

G. Fanizza ◽

L. Ricciardi ◽

D. Boscia ◽

O. Silvestroni

Keyword(s):

Vitis Vinifera ◽

High Temperatures ◽

Shoot Tip ◽

Vitis Vinifera L ◽

Root Induction ◽

Shoot Tip Culture

Download Full-text

An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte

Plants ◽

10.3390/plants9020235 ◽

2020 ◽

Vol 9 (2) ◽

pp. 235 ◽

Cited By ~ 1

Author(s):

Kyungtae Park ◽

Bo Kook Jang ◽

Ha Min Lee ◽

Ju Sung Cho ◽

Cheol Hee Lee

Keyword(s):

Nitrogen Sources ◽

Culture Conditions ◽

Shoot Tips ◽

Shoot Tip ◽

Soil Conditions ◽

Ms Medium ◽

Ex Vitro ◽

Shoot Tip Culture ◽

Propagation Methods

Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation methods for this species have not been developed. We aimed to develop an efficient propagation method for S. martensii through in vitro culture. We investigated culture conditions that are suitable for shoot-tip proliferation and growth. The optimum shoot-tip culture conditions were determined while using Murashige and Skoog (MS) medium (quarter, half, full, or double strength) and macronutrients (sucrose and two nitrogen sources) at various concentrations. In MS medium, the shoot tips formed a maximum of 6.77 nodes per explant, and each node formed two new shoot tips (i.e., 26 or 64 shoot tips). When using branching segments containing an angle meristem, the shoot-to-rhizophore formation ratio could be controlled by medium supplementation with plant-growth regulators. Sporophytes that were grown from shoot tips in vitro were acclimated in ex vitro soil conditions and successfully survived in the greenhouse. Numerous shoot tips could be obtained from in vitro-grown sporophytes and be proliferated ex vitro to produce a large number of plants. This method provides a way of shortening the time that is required for producing a large stock of S. martensii planting material.

Download Full-text