Virus resistance of transgenic potato plants (Solanum tuberosum L.) bearing PVY NTN -CP gene of potato virus Y coat protein

2011 ◽  
Vol 1 (2) ◽  
pp. 103-111
Author(s):  
Z. Stashevski ◽  
O. N. Il’inskaya
Keyword(s):  
Solanum Tuberosum ◽  
Coat Protein ◽  
Potato Virus ◽  
Virus Resistance ◽  
Potato Virus Y ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Cp Gene ◽  
Transgenic Potato Plants

scholarly journals PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants

2009 ◽  
Vol 7 (4) ◽  
pp. 41-50 ◽  
Author(s):  
Zenon Stasevski ◽  
Olga N Ilinskaya
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Transgenic Potatoes ◽  
Virus Strains

PVY<sup style="line-height:1.6em">NTN</sup>-CP <span style="line-height:1.6em">coat protein gene from a necrotic strain of potato virus </span>Y (pvy<sup style="line-height:1.6em">ntn</sup>) <span style="line-height:1.6em">has been transferred into two potato </span>Solanum tuberosum L. <span style="line-height:1.6em">cultivars </span>Mindenes <span style="line-height:1.6em">and </span>Somogyi kifli via Agrobacterium tumefaciens <span style="line-height:1.6em">transformation. Expression of integrated PVY</span><sup style="line-height:1.6em">NTN</sup><span style="line-height:1.6em">-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains </span>(PVY°, PVY<sup style="line-height:1.6em">NTN</sup>) <span style="line-height:1.6em">of independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains.</span>

scholarly journals Extreme resistance to Potato Virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor

2018 ◽  
Author(s):  
Marta Grech-Baran ◽  
Kamil Witek ◽  
Katarzyna Szajko ◽  
Agnieszka I Witek ◽  
Karolina Morgiewicz ◽  
...  
Keyword(s):  
Single Molecule ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Resistance Breeding ◽  
Transgenic Potato ◽  
Extreme Resistance ◽  
Breeding Programs ◽  
Potato Plants ◽  
Gene Enrichment ◽  
Transgenic Potato Plants

ABSTRACTPotato virus Y (PVY) is a major potato pathogen that causes annual losses of billions of dollars. Control of its transmission requires extensive use of environmentally damaging insecticides. Rysto confers extreme resistance (ER) to PVY and is a valuable trait in resistance breeding programs. We isolated Rysto using Resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences Single-Molecule Real Time Sequencing). Rysto encodes a nucleotide binding-leucine rich repeat (NLR) protein with an N-terminal TIR domain, and is sufficient for PVY perception and extreme resistance in transgenic potato plants. We investigated the requirements for Rysto-dependent extreme resistance, and showed that Rysto function is temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.

Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing

2004 ◽  
Vol 14 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Anastasia Missiou ◽  
Kriton Kalantidis ◽  
Alexandra Boutla ◽  
Sergia Tzortzakaki ◽  
Martin Tabler ◽  
...  
Keyword(s):  
Potato Virus ◽  
Rna Silencing ◽  
Potato Virus Y ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants

1992 ◽  
Vol 11 (1) ◽  
Author(s):  
Attila Feh�r ◽  
KonstantinG. Skryabin ◽  
Ervin Bal�zs ◽  
Johanna Preiszner ◽  
OlgaA. Shulga ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Gene Promoter ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

Transgenic potato plants expressing the potato virus X (PVX) coat protein gene developed resistance to the viral infection

2002 ◽  
Vol 30 (2) ◽  
pp. 177-185 ◽  
Author(s):  
V. Doreste ◽  
P. L. Ramos ◽  
G. A. Enríquez ◽  
R. Rodríguez ◽  
R. Peral ◽  
...  
Keyword(s):  
Coat Protein ◽  
Viral Infection ◽  
Potato Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Virus X ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

scholarly journals Obtaining of transgenic potato plants (Solanum tuberosum L.) that contain antisense sequence of prolindehydrogenase gene

2018 ◽  
Vol 22 ◽  
pp. 299-304
Author(s):  
O. O. Ovcharenko ◽  
V. A. Rudas ◽  
N. L. Shcherbak ◽  
M. V. Kuchuk
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Practical Interest ◽  
Transgenic Potato ◽  
Proline Dehydrogenase ◽  
Potato Plants ◽  
Antisense Sequence ◽  
Transgenic Potato Plants

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.

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