scholarly journals Manipulation of carbon flux into fatty acid biosynthesis pathway in Dunaliella salina using AccD and ME genes to enhance lipid content and to improve produced biodiesel quality

2014 ◽  
pp. 91-97 ◽  
Author(s):  
Ahmad Farhad Talebi ◽  
Masoud Tohidfar ◽  
Abdolreza Bagheri ◽  
Stephen R. Lyon ◽  
Kourosh Salehi-Ashtiani ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Carbon Flux ◽  
Dunaliella Salina ◽  
Fatty Acid Biosynthesis ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Fatty Acid Biosynthesis Pathway ◽  
Biodiesel Quality

scholarly journals Reconstruction of the Fatty Acid Biosynthetic Pathway ofExiguobacterium antarcticumB7 Based on Genomic and Bibliomic Data

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Regiane Kawasaki ◽  
Rafael A. Baraúna ◽  
Artur Silva ◽  
Marta S. P. Carepo ◽  
Rui Oliveira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Short Chain Fatty Acids ◽  
Hexadecenoic Acid ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Cold Environments ◽  
Fatty Acid Biosynthesis Pathway

Exiguobacterium antarcticumB7 is extremophile Gram-positive bacteria able to survive in cold environments. A key factor to understanding cold adaptation processes is related to the modification of fatty acids composing the cell membranes of psychrotrophic bacteria. In our study we show thein silicoreconstruction of the fatty acid biosynthesis pathway ofE. antarcticumB7. To build the stoichiometric model, a semiautomatic procedure was applied, which integrates genome information using KEGG and RAST/SEED. Constraint-based methods, namely, Flux Balance Analysis (FBA) and elementary modes (EM), were applied. FBA was implemented in the sense of hexadecenoic acid production maximization. To evaluate the influence of the gene expression in the fluxome analysis, FBA was also calculated using thelog2⁡FCvalues obtained in the transcriptome analysis at 0°C and 37°C. The fatty acid biosynthesis pathway showed a total of 13 elementary flux modes, four of which showed routes for the production of hexadecenoic acid. The reconstructed pathway demonstrated the capacity ofE. antarcticumB7 tode novoproduce fatty acid molecules. Under the influence of the transcriptome, the fluxome was altered, promoting the production of short-chain fatty acids. The calculated models contribute to better understanding of the bacterial adaptation at cold environments.

Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli

2014 ◽  
Vol 99 (4) ◽  
pp. 1795-1804 ◽  
Author(s):  
Xiping Liu ◽  
Haiying Yu ◽  
Xu Jiang ◽  
Guomin Ai ◽  
Bo Yu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Butenoic Acid ◽  
Fatty Acid Biosynthesis Pathway
Sign in / Sign up

Export Citation Format

Share Document