scholarly journals Effects of autologous platelet-rich plasma on endometrial expansion in patients undergoing frozen-thawed embryo transfer: A double-blind RCT

2019 ◽  
Author(s):  
Leila Nazari ◽  
Saghar Salehpour ◽  
Sedighe Hoseini ◽  
Shahrzad Zadehmodarres ◽  
Eznoallah Azargashb
Keyword(s):  
Embryo Transfer ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Double Blind ◽  
Thin Endometrium ◽  
Catheter Group ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Background: Adequate endometrial growth is principal for implantation and pregnancy. Thin endometrium is associated with lower pregnancy rate in assisted reproductive technology. Some frozen-thawed embryo transfer cycles are cancelled due to inadequate endometrial growth. Objective: To assess the effectiveness of autologous platelet-rich plasma (PRP) intrauterine infusion for the treatment of thin endometrium. Materials and Methods: A total of 72 patients who had a history of cancelled frozen-thawed embryo transfer cycle due to the thin endometrium (< 7mm) were assessed for the eligibility to enter the study between 2016 and 2017. Twelve patients were excluded for different reasons, and 60 included patients were randomly assigned to PRP or sham-catheter groups in a double-blind manner. Hormone replacement therapy was administered for endometrial preparation in all participants. PRP intrauterine infusion or sham-catheter was performed on day 11-12 due to the thin endometrium and it was repeated after 48 hr if necessary. Results: Endometrial thickness increased at 48 hr after the first intervention in both groups. All participants needed second intervention due to an inadequate endometrial expansion. After second intervention, endometrial thickness was 7.21 ± 0.18 and 5.76 ± 0.97 mm in the PRP group and sham-catheter group, respectively. There was a significant difference between the two groups. (p < 0.001). Embryo transfer was done for all patients in PRP group and just in six cases in the sham-catheter group. Chemical pregnancy was reported in twelve cases in the PRP group and two cases in the sham-catheter group. Conclusion: According to this trial, PRP was effective in endometrial expansion in patients with refractory thin endometrium.

scholarly journals Effect of autologous platelet rich plasma on thin endometrium in frozen thawed embryo transfer

2020 ◽  
Vol 9 (3) ◽  
pp. 1079
Author(s):  
Adaboina Anitha ◽  
Burri Sandhya Rani
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Study Data ◽  
Thin Endometrium ◽  
History Of ◽  
Autologous Platelet Rich Plasma ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Background: Endometrium is one of the main factors in implantation and pregnancy. Some of the assisted reproductive technology treatment (ART) cycles get cancelled due to inadequate endometrial growth. This study was conducted to evaluate the effectiveness of PRP (platelet rich plasma) in the treatment of thin endometrium and its outcome on pregnancy and live birth rates.Methods: This study was a prospective cohort which was conducted from January 2018 to December 2018 at Laxmi Narasimha Hospital, Warangal, Telangana State. 30 patients with history of inadequate endometrial growth in frozen thawed embryo transfer cycles were recruited into the study. Intrauterine infusion of PRP was performed. Endometrial thickness was assessed.Results: 30 women were recruited in the study data of the 24 women were collected. Live birth was seen in 5 patients in the week range of 37±to 37±5 and average EMT in mm was 7.8, abortion was seen in 3 patients in the week range of 6 to 8±2 and average EMT in mm was 7.4 , chemical pregnancy was seen in 2 patients in the week range of 6 and average EMT in mm was 7.2 and number of women who were not pregnant were 14 and average EMT in mm was 7.0. Live birth was observed in 21% of the patients, abortion was observed in 13%, chemical pregnancy was seen in 9% and women who were not pregnant were 58%.Conclusions: Ability of autologous PRP to restore the endometrial receptivity of damaged endometrium has some aspects other than increasing the EMT.

scholarly journals Treatment of unexplained thin endometrium with autologous platelet-rich plasma in a frozen embryo transfer cycle

2019 ◽  
Vol 2 (2) ◽  
pp. 80-82
Author(s):  
Sara Mahmood ◽  
Salma Kafeel ◽  
Riffat Bibi ◽  
Naveed Iqbal
Keyword(s):  
Embryo Transfer ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Uterine Cavity ◽  
Blastocyst Stage ◽  
Frozen Embryo Transfer ◽  
Thin Endometrium ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet ◽  
Endometrial Proliferation

Inadequate endometrial proliferation is a known cause of implantation failure in assisted reproductive technology cycles. It is generally agreed that >9 mm endometrial thickness is associated with higher implantation. Several strategies have been explored to enhance endometrial proliferation. However, the results are either poor, inconsistent or subject to safety concerns. A 34-year old woman presented with unexplained thin endometrium in successive frozen embryo transfer (FET) cycles. Autologous platelet-rich plasma (PRP) was infused in the uterine cavity on day 10 of second FET cycle enhancing endometrial thickness, which post-PRP infusion measured 10.9 mm. Blastocyst-stage embryos were transferred resulting in a successful pregnancy. Autologous intrauterine infusion of PRP positively impacts endometrial proliferation and implantation which is safe, low resource and minimally invasive.

Effectiveness of intrauterine administration of autologous platelet-rich plasma for the preparation of the "thin" endometrium for the program of defrosted embryo transfer

2021 ◽  
Vol 2_2021 ◽  
pp. 90-95
Author(s):  
Dzhincharadze L.G. Dzhincharadze ◽  
Abubakirov A.N. Abubakirov ◽  
Mishieva N.G. Mishieva ◽  
Bakuridze E.M. Bakuridze ◽  
Bystrykh O.A. Bystrykh ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Platelet Rich Plasma ◽  
Thin Endometrium ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

P–301 Intrauterine instillation of autologous platelet rich plasma for thin endometrium improves the outcome of frozen embryo transfer cycles

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Nagireddy ◽  
S R Nellepalli ◽  
R Vembu ◽  
M Pandurangi ◽  
M Gopal. Krishnan ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Estradiol Valerate ◽  
Group A ◽  
Autologous Platelet Rich Plasma ◽  
Group B ◽  
Study Participants ◽  
Autologous Platelet

Abstract Study question How does the intrauterine instillation of autologous platelet rich plasma (PRP) affect the endometrial thickness and live birth rate in frozen embryo transfer cycles? Summary answer Intrauterine instillation of autologous PRP resulted in significant improvement in endometrial thickness. The live birth rates were satisfactory post-PRP instillation. What is known already Autologous Platelet rich plasma (PRP) had resulted in significant improvement in endometrial thickness, when instilled intrauterine in women with thin endometrium in FET cycles. Study design, size, duration A retrospective observational study was performed at a tertiary care university teaching hospital in South India. 35 women who received intrauterine autologous PRP during endometrial preparation for frozen embryo transfer from June 2017 to December 2020, were included. Patients who underwent donor oocyte recipent cycles, those with a history of tubercular endometritis, Asherman syndrome, previous intrauterine manipulations such as manual removal of placenta, and uterine anomalies were excluded. Participants/materials, setting, methods All the women underwent endometrial preparation in artificial cycles by depot GnRH agonist suppression and HRT (Hormone replacement therapy) was initiated by 4–6 mg of estradiol valerate and stepped up as required. Autologous PRP was offered to all women who had endometrial thickness &lt; 7 mm on day 16 of HRT. PRP was prepared by the two-step centrifugation method and administered intrauterine by IUI catheter. The patients underwent repeat evaluation after 5 days post-PRP instillation. Main results and the role of chance Optimal response to PRP was considered as the attainment of an endometrial thickness (ET) ≥ 7mm after 5 days of post-PRP. 25 (71.4%) had an optimal response to PRP. There was a significant improvement in the endometrial thickness(mm) in the study participants following PRP instillation: 6.3 ± 0.6 vs. 7.1 ± 1.2; P = 0.0001.The study participants were divided into two groups based on their response to intrauterine PRP instillation. Those who optimally responded to PRP were categorized as Group A and those who didn’t were categorized as Group B. The study participants of both the groups were comparable by their demographic characteristics such as age, cause of infertility, indications for ART, and the dose of estradiol valerate before PRP. The dose of estradiol valerate (mg) after PRP was significantly higher in Group B compared to Group A: 19.9 ± 4.9 vs. 15.6 ± 3.9; P = 0.014. A total of 26 women underwent embryo transfer and 9 (25.7%) women had cycle cancellation. Of these 22 were from Group A and 4 from Group B. The pregnancy, clinical pregnancy, miscarriage and live birth rates were 36.3%(8/22) and 25% (1/4); 31.8% (7/22) and 25% (1/4); and 31.8% (7/22) and 25% (1/4), respectively. Limitations, reasons for caution As the study was retrospective in nature and the PRP was offered only in patients who had consented, there was a significant bias. Hence the results of the study should be interpreted with caution. Further large prospective RCTs (Randomised controlled trials) are required to confirm our findings. Wider implications of the findings: Autologous PRP may enhance the response to the estrogen preparations. It may produce satisfactory live birth rates and reduce cycle cancellations in a reasonable proportion of patients with thin endometrium in FET cycles. However, these findings should be confirmed by dose finding clinical trials, and studies involving a comparison group. Trial registration number Not applicable

Sign in / Sign up

Export Citation Format

Share Document