scholarly journals In-situ Alkaline Transesterification of Jatropha curcas seed Oil for Production of Biodiesel and Nontoxic Jatropha seed Cake

Author(s):  
Novizar Nazir ◽  
Djumali Mangunwidjaja ◽  
Dwi Setyaningsih ◽  
Sri Yuliani ◽  
Mohd. Ambar Yarmo ◽  
...  
2013 ◽  
Vol 5 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Amina M. AHMED EL-IMAM ◽  
Muinat O. KAZEEM ◽  
Mutiat B. ODEBISI ◽  
Mushaffa A. OKE ◽  
Azeezat O. ABIDOYE

Submerged substrate fermentation of Jatropha seed cake, a by-product of oil extraction from Jatropha curcas seed was carried out using Aspergillus terreus for the production of itaconic acid. The Jatropha seed cake was initially converted into fermentable sugars by dilute acid hydrolysis using 50% sulphuric acid. The rate of hydrolysis was 1.04 gL-1. The fermentation process was carried out at room temperature, agitation of 400 rpm and three physico-chemical parameters (pH, inoculum size and substrate concentration) were varied. Itaconic acid and glucose assays were carried out by spectrophotometry and Dinitrosalicylic acid methods respectively daily. Maximum yield of itaconic acid was 48.70 gL-1 at 5 ml of inoculum size, 50 g substrate concentration and pH 1.5. The residual glucose concentration increased for the first two days of fermentation after which it began to decrease as the itaconic acid concentration increased. The least concentration of itaconic acid observed was 6.00 gL-1, obtained after 24 hours of fermentation with 4 ml inoculum size, 50 g substrate concentration and at pH 1.5. The findings of this work indicate that Jatropha curcas seed cake is a suitable substrate for itaconic acid production.


2017 ◽  
Vol 20 (2) ◽  
pp. 108 ◽  
Author(s):  
Chusnul Hidayat ◽  
Sari Darmasiwi ◽  
Maulina Nurikasari ◽  
Muhammad Nur Cahyanto

Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potentialas a medium for lipase production. The objective of this research was to characterize lipase from Aspergillusniger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specifcity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specifcity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and vmax about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.


2019 ◽  
Vol 26 (2) ◽  
pp. 102
Author(s):  
M.A. Obianke ◽  
A.B. Muhammad ◽  
L.G. Hassan ◽  
A.A. Aliero ◽  
M.G. Liman

2021 ◽  
Vol 27 (4) ◽  
pp. 33-45
Author(s):  
Ejiro Thelma Akhihiero ◽  
Bamidele Victor Ayodele ◽  
May Ali Alsaffar ◽  
T. O.K. Audu ◽  
E. O. Aluyor

The world is confronted with the twin crisis of fossil fuel depletion and environmental degradation caused by fossil fuel usage. Biodiesel produced from renewable feedstocks such as Jatropha seed oil or animal fats by transesterification offers a solution. Although biodiesel has been produced from various vegetable oils such as Jatropha seed oil, the reaction kinetics studies are very few in literature, hence the need for this study. Jatropha curcas seed oil was extracted and analyzed to determine its free fatty acid and fatty acid composition. The oil was transesterified with methanol at a molar ratio of methanol to oil 8:1, using 1% sodium hydroxide catalyst, at different temperatures ranging from 32oC to 65oC, at atmospheric pressure. The order of the reactions with respect to the triglyceride's disappearance in the forward reaction at the chosen temperatures was found to be pseudo-first-order and found to be first-order for the reaction at 32oC. The rate constants of the three consecutive reaction steps at 65oC, namely, triglyceride to diglyceride, diglyceride to monoglyceride, and monoglyceride to glycerol, were found to be 0.422 min-1 0.117 min-1, and 0.037min-1, respectively. Their corresponding activation energies in J/mol were 22.165, 3.136, and 19.770, respectively.


Sign in / Sign up

Export Citation Format

Share Document