Influence of freezing temperature on semen quality of rainbow trout (Oncorhynchus mykiss)

Trout production in Peru has export potential. However, there are no fry available to maintain production throughout the year and there is also a lack of egg production at certain times of the year. Therefore, the alternative is to cryopreserve semen for the non-reproductive season. The objective of the study was to determine the influence of freezing temperature on the quality of rainbow trout semen. The semen of 12 reproductive males was collected in the facilities of the Chucuito Research and Production Center of the National University of the Altiplano of Puno, which underwent a macroscopic, microscopic pre and post thaw seminal evaluation and the fertility rate was measured. Due to the effect of three freezing temperatures (-80 °C, -100 °C, -120 °C), cryopreservation had a decline curve of -20 °C / min. The fresh seminal parameters were similar to those reported by other researchers. While freezing had unfavorable effects on semen quality, the best results for activation time (51.33 sec) and vitality (35.33%) were obtained with -100 °C, but the higher motility was obtained with -120 °C (36.33%). Regarding fertility, the higher rate was obtained with -100 °C (70.97%), followed by -80 °C and -120 °C in which 68.86% and 64.34% were obtained, respectively. In conclusion, the results suggest that the tolerable freezing temperature of rainbow trout semen is around -100 °C, which is shown as a favorable alternative for the reproductive management of rainbow trout under natural hypobaric conditions of the Peruvian highlands

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The influence of various cryoprotectants on semen quality of the rainbow trout (Oncorhynchus mykiss) before and after cryopreservation

Journal of Applied Ichthyology ◽

10.1111/j.1439-0426.1996.tb00070.x ◽

1996 ◽

Vol 12 (2) ◽

pp. 99-106 ◽

Cited By ~ 55

Author(s):

F. Lahnsteiner ◽

B. Berger ◽

T. Weismann ◽

R. Patzner

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Semen Quality ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Before And After

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Effect of Ergosan on semen quality of male rainbow trout (Oncorhynchus mykiss) broodstock

Animal Reproduction Science ◽

10.1016/j.anireprosci.2010.08.008 ◽

2010 ◽

Vol 122 (3-4) ◽

pp. 183-188 ◽

Cited By ~ 6

Author(s):

Najmeh Sheikhzadeh ◽

Asadpour Reza ◽

Jafari Jozani Razi Allah ◽

Tayefi-Nasrabadi Hossein

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Semen Quality ◽

Rainbow Trout Oncorhynchus Mykiss

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Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism

Aquaculture ◽

10.1016/s0044-8486(98)00243-9 ◽

1998 ◽

Vol 163 (1-2) ◽

pp. 163-181 ◽

Cited By ~ 231

Author(s):

F Lahnsteiner ◽

B Berger ◽

T Weismann ◽

R.A Patzner

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Sperm Motility ◽

Seminal Plasma ◽

Semen Quality ◽

Plasma Parameters ◽

Rainbow Trout Oncorhynchus Mykiss

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Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability

BMC Genomics ◽

10.1186/s12864-021-07773-1 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Gregory M. Weber ◽

Jill Birkett ◽

Kyle Martin ◽

Doug Dixon ◽

Guangtu Gao ◽

...

Keyword(s):

Rainbow Trout ◽

Egg Quality ◽

Transcript Abundance ◽

Differentially Expressed ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Analysis Group ◽

Maternal Transcripts ◽

Single Batch ◽

Insight Into

Abstract Background Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo. The transcriptome of the unfertilized egg, therefore, has potential to provide markers for predictors of egg quality and diagnosing problems with embryo production encountered by fish hatcheries. Although levels of specific transcripts have been shown to associate with measures of egg quality, these differentially expressed genes (DEGs) have not been consistent among studies. The present study compares differences in select transcripts among unfertilized rainbow trout eggs of different quality based on eyeing rate, among 2 year classes of the same line (A1, A2) and a population from a different hatchery (B). The study compared 65 transcripts previously reported to be differentially expressed with egg quality in rainbow trout. Results There were 32 transcripts identified as DEGs among the three groups by regression analysis. Group A1 had the most DEGs, 26; A2 had 15, 14 of which were shared with A1; and B had 12, 7 of which overlapped with A1 or A2. Six transcripts were found in all three groups, dcaf11, impa2, mrpl39_like, senp7, tfip11 and uchl1. Conclusions Our results confirmed maternal transcripts found to be differentially expressed between low- and high-quality eggs in one population of rainbow trout can often be found to overlap with DEGs in other populations. The transcripts differentially expressed with egg quality remain consistent among year classes of the same line. Greater similarity in dysregulated transcripts within year classes of the same line than among lines suggests patterns of transcriptome dysregulation may provide insight into causes of decreased viability within a hatchery population. Although many DEGs were identified, for each of the genes there is considerable variability in transcript abundance among eggs of similar quality and low correlations between transcript abundance and eyeing rate, making it highly improbable to predict the quality of a single batch of eggs based on transcript abundance of just a few genes.

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