Phytochrome A and phytochrome B regulate the biosynthesis of chlorophyll during cytokinin-dependent de-etiolation of Arabidopsis thaliana

AbstractNitrogenous compounds, such as potassium nitrate, potentiate germination of different species of light-requiring seeds. Using light-induced Arabidopsis thaliana seed germination as a model system, our data suggested that only phytochrome A (phyA)-specific induced germination was affected after the exogenous application of nitrates, different nitric oxide (NO)-donors (such as organic nitrates) or sodium nitroprusside. The stimulative effect was very pronounced. Treated seed samples reached maximal germination after very short periods of red-light irradiation. To a far lesser extent, these substances affected phytochrome B (phyB)-specific induced germination. In phyB-specific induced germination, potassium nitrate was most effective, but germination percentages never exceeded 50%. The least effective was sodium nitroprusside, which practically did not affect phyB-specific induced germination. These results were confirmed using corresponding phytochrome mutants.

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The Induction of Seed Germination in Arabidopsis thaliana Is Regulated Principally by Phytochrome B and Secondarily by Phytochrome A

PLANT PHYSIOLOGY ◽

10.1104/pp.104.2.363 ◽

1994 ◽

Vol 104 (2) ◽

pp. 363-371 ◽

Cited By ~ 143

Author(s):

T. Shinomura ◽

A. Nagatani ◽

J. Chory ◽

M. Furuya

Keyword(s):

Arabidopsis Thaliana ◽

Seed Germination ◽

Phytochrome B ◽

Phytochrome A

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Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings

Physiologia Plantarum ◽

10.1111/j.1399-3054.1997.tb00997.x ◽

1997 ◽

Vol 101 (2) ◽

pp. 278-282 ◽

Cited By ~ 31

Author(s):

Abdul-kader Janoudi ◽

Radomir Konjevic ◽

Garry Whitelam ◽

William Gordon ◽

Kenneth L. Poff

Keyword(s):

Arabidopsis Thaliana ◽

Phytochrome B ◽

Phytochrome A ◽

Normal Expression

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Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

Plant and Cell Physiology ◽

10.1093/pcp/pci073 ◽

2005 ◽

Vol 46 (5) ◽

pp. 790-796 ◽

Cited By ~ 8

Author(s):

Aurora Piñas Fernández ◽

Patricia Gil ◽

Ildiko Valkai ◽

Ferenc Nagy ◽

Eberhard Schäfer

Keyword(s):

Arabidopsis Thaliana ◽

Nicotiana Plumbaginifolia ◽

Phytochrome B

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Light Gradient-Based Screening of Arabidopsis thaliana on a 384-Well Type Plant Array Chip

Micromachines ◽

10.3390/mi11020191 ◽

2020 ◽

Vol 11 (2) ◽

pp. 191

Author(s):

Youn-Hee Park ◽

Je-Kyun Park

Keyword(s):

Arabidopsis Thaliana ◽

White Light ◽

Solid Medium ◽

Red Light ◽

Phytochrome B ◽

Wild Type ◽

Light Gradient ◽

Gradient Based ◽

Light Effects ◽

Germination And Growth

Arabidopsis thaliana (Arabidopsis), as a model for plant research, is widely used for various aspects of plant science. To provide a more sophisticated and microscopic environment for the germination and growth of Arabidopsis, we report a 384-well type plant array chip in which each Arabidopsis seed is independently seeded in a solid medium. The plant array chip is made of a poly(methyl methacrylate) (PMMA) acrylic material and is assembled with a home-made light gradient module to investigate the light effects that significantly affect the germination and growth of Arabidopsis. The light gradient module was used to observe the growth pattern of seedlings according to the intensity of the white light and to efficiently screen for the influence of the white light. To investigate the response to red light (600 nm), which stimulates seed germination, the light gradient module was also applied to the germination test. As a result, the germination results showed that the plant array chip can be used to simultaneously screen wild type seeds and phytochrome B mutant seeds on a single array chip according to the eight red light intensities.

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