scholarly journals Study on Antifungal Activity and Ability Against Rice Leaf Blast Disease of Nano Cu-Cu2O/Alginate

2020 ◽  
Author(s):  
Doan Thi Bich Ngoc ◽  
Bui Duy Du ◽  
Le Nghiem Anh Tuan ◽  
Bui Dinh Thach ◽  
Chu Trung Kien ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Untreated Control ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Leaf Blast ◽  
Cu Content ◽  
Polished Rice ◽  
Rice Leaf Blast

Background: Rice blast disease due to Pyricularia oryzae fungus is one of the most destructive ones for rice, causing serious losses in yield and quality in Vietnam and other countries. Studying to develop new fungicides to control the fungal disease effectively is essential.Methods: The sodium alginate-stabilized Cu-Cu2O nanocolloidal solution with a size of about 4 nm and the Cu concentration of 5,000 mg/L was prepared by chemical method. The antifungal activity in vitro against P. oryzae and the ability to control the rice blast disease in the greenhouse of nano Cu-Cu2O/alginate were assessed.Result: The nano Cu-Cu2O/alginate exhibited highly fungal activity with the IC50 of 17.8 mg Cu /L. The disease severity of nano Cu-Cu2O/alginate treatments was in the range of 9.38 - 18.54% in comparison with 47.57% of the untreated control. The grain yield of rice plants treated with nano Cu-Cu2O/alginate increased compared with that of the untreated control, and the Cu content in polished rice was almost the same as that of usually cultured polished rice. Thus, the nano Cu-Cu2O/alginate can be used in agriculture as a plant fungicide, especially for rice cultivation.

scholarly journals PAF104, a Synthetic Peptide to Control Rice Blast Disease by Blocking Appressorium Formation in Magnaporthe oryzae

2013 ◽  
Vol 26 (12) ◽  
pp. 1407-1416 ◽  
Author(s):  
Aarón Rebollar ◽  
Belén López-García
Keyword(s):  
Magnaporthe Oryzae ◽  
Synthetic Peptide ◽  
Rice Blast ◽  
Surface Protein ◽  
Inhibitory Effect ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Appressorium Formation ◽  
Hydrophobic Surfaces

Magnaporthe oryzae is the most devastating pathogen of rice and the main cause of crop losses worldwide. The successful management of blast disease caused by this fungus is a clear necessity. The synthetic peptide PAF104 has been characterized by its inhibition of M. oryzae appressorium formation on hydrophobic surfaces. Growth and the ability of conidia to germinate was not affected by PAF104, indicating the lack of toxicity on fungal conidia. The addition of the cutin monomer 1,16-hexadecanediol does not interfere with the inhibitory effect of PAF104 on in vitro hydrophobic surfaces. On the other hand, inhibition of appressorium formation by PAF104 was nullified by the exogenous addition of cAMP. Our results suggest that PAF104 affects the Pmk1 pathway by repression of the gene expression of MoMSB2, which encodes a sensing surface protein, and the mitogen-activated protein/extracellular signal-regulated kinase kinase kinase MST11. The pathogenicity of M. oryzae was reduced after PAF104 treatment specifically blocking appressorium formation. Our results support PAF104 as a promising compound to control rice blast disease by blocking a specific target related to appressorium formation, a process essential for infection of rice leaves. Moreover, PAF104 is proposed as a lead compound to develop novel specific fungicides with improved properties.

scholarly journals Evaluation of Biocontrol Activities of Streptomyces spp. against Rice Blast Disease Fungi

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 126 ◽  
Author(s):  
Mathurot Chaiharn ◽  
Teerayut Theantana ◽  
Wasu Pathom-aree
Keyword(s):  
Rice Blast ◽  
Dry Weight ◽  
Rice Cultivar ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Streptomyces Spp ◽  
Commercially Important

Rhizosphere bacteria can positively influence plant growth by direct and indirect mechanisms. A total of 112 bacterial strains were isolated from the rhizosphere of rice and tested for plant beneficial activities such as siderophore production, cell-wall-degrading enzyme production, hydrogen cyanide (HCN) production and antifungal activity against rice blast disease fungus. The actinomycetes count was 3.8 × 106 CFU/g soil. Streptomyces strains PC 12, D 4.1, D 4.3 and W1 showed strong growth inhibition of blast disease fungus, Pyricularia sp. (87.3%, 82.2%, 80.0% and 80.5%) in vitro. Greenhouse experiments revealed that rice plants treated with Streptomyces strain PC 12 recorded maximum plant height, root length and root dry weight compared to the control. Taxonomic characterization of this strain on the basis of 16S rRNA gene sequence led to its identification as Streptomyces palmae PC 12. Streptomyces palmae PC 12 may be used as biofertilizer to enhance the growth and productivity of commercially important rice cultivar RD6 and the biocontrol of blast disease fungus.

scholarly journals Plant tonic, a plant-derived bioactive natural product, exhibits antifungal activity against rice blast disease

2018 ◽  
Vol 112 ◽  
pp. 105-112 ◽  
Author(s):  
Farnaz Abed-Ashtiani ◽  
Mahdi Arzanlou ◽  
Abbas Nasehi ◽  
Jugah Kadir ◽  
Ganesan Vadamalai ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Natural Product ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Bioactive Natural Product

scholarly journals Characterization of rue extract and its potential for controlling rice blast

2015 ◽  
Vol 50 (12) ◽  
pp. 1121-1130 ◽  
Author(s):  
Karinna Bannach Reis ◽  
Marcio Vinicius de Carvalho Barros Côrtes ◽  
Frederico Severino Martins ◽  
Marta Cristina Corsi de Filippi ◽  
José Realino de Paula ◽  
...  
Keyword(s):  
Rice Blast ◽  
Mycelial Growth ◽  
Integrated Management ◽  
Conidial Germination ◽  
Appressorium Formation ◽  
Leaf Blast ◽  
Liquid Extract ◽  
Rice Leaf Blast

Abstract: The objective of this work was to purify and standardize the rue (Ruta graveolens) extract and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.

scholarly journals Identificação de progênies de arroz resistentes à brusone no Estado de São Paulo, em 1976/77

Bragantia ◽  
1985 ◽  
Vol 44 (1) ◽  
pp. 311-329
Author(s):  
Jaciro Soave ◽  
Luiz Ernesto Azzini ◽  
Octávio Bento de Almeida Camargo ◽  
Armando Pettinelli Júnior ◽  
Mauro Sakai
Keyword(s):  
Rice Blast ◽  
Sao Paulo ◽  
Pyricularia Oryzae ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
São Paulo

Este trabalho apresenta os resultados das pesquisas realizadas para a avaliação da resistência à brusone (Pyricularia oryzae Cav.) dos principais materiais de sequeiro e irrigado do programa de melhoramento genético do Instituto Agronômico do Estado de São Paulo, e de genótipos exóticos, introduzidos de diversos países, visando à obtenção de cultivares de arroz resistentes àquela limitante doença fúngica. Os testes foram realizados em condições de campo, em canteiros padronizados para reação uniforme a P. oryzae, e a avaliação das plantas foi feita através da observação dos sintomas visuais deixados pela doença, aos quais foram atribuídas notas de 1 a 7, conforme a escala de notas adotadas no "Symposium on the rice blast disease", em 1963. Sessenta e três germoplasmas de arroz de sequeiro e trinta de cultivo irrigado foram testados quanto à resistência à brusone na folha, nas seguintes localidades paulistas: Itararé, Mococa, Pariquera-Açu, Pindamonhangaba, Pindorama e Ribeirão Preto. Foram ainda avaliados 102 genótipos exóticos de arroz visando à detecção de fontes de resistência à brusone nas mesmas localidades, além de Campinas. Somente cinco cultivares de sequeiro, GS-73-164, GS-73-165, GS-73-94, IAC-25 e GS-73-17, e dois cultivares de arroz irrigado, IAC-120 e Pinda F-3-7, embora suscetíveis, apresentaram comportamento satisfatório quanto à brusone. Dos genótipos exóticos testados, vinte e sete foram indicados como fontes de resistência à brusone no Estado de São Paulo.

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