Bioinformatics Analysis of the Duck Enteritis Virus UL54 Gene

It is generally recognised that bioinformatics analysis plays a important role in the study of genes and proteins. Here in this article we intend to report some bioinformation about the nucleotide sequence of DEV(Duck virus enteritis）US2 gene.We used DNAstar7.1 software、Primer5.0 and some online websites (e.g.NCBI、BepiPred)to analysis the potential bioinformation in nucleotide of this gene. These results indicated that DEV should be placed in a single cluster within the subfamily Alphaherpesvirinae,and nucleotide sequence of DEV-CHv US2 have 100% similarities with other six strains of DEV US2 gene sequences with sequence alignment and so on.To date,little information on DEV US2 gene has been found,so we provide some materials information for further research.

Download Full-text

Bioinformatics Analysis and Characteristics of Glycoprotein K Encoded by the Newly Identified UL53 Gene of Duck Enteritis Virus

2010 4th International Conference on Bioinformatics and Biomedical Engineering ◽

10.1109/icbbe.2010.5515578 ◽

2010 ◽

Author(s):

Shunchuan Zhang ◽

Anchun Cheng ◽

Mingshu Wang ◽

Jun Xiang ◽

Dekang Zhu ◽

...

Keyword(s):

Bioinformatics Analysis ◽

Duck Enteritis Virus ◽

Glycoprotein K ◽

Ul53 Gene ◽

Enteritis Virus

Download Full-text

Bioinformatics analysis and characteristics of glycoprotein G encoded by the newly identified Us4 gene of duck enteritis virus

2010 3rd International Conference on Biomedical Engineering and Informatics ◽

10.1109/bmei.2010.5639299 ◽

2010 ◽

Author(s):

Xiaoyuan Yang ◽

Anchun Cheng ◽

Mingshu Wang ◽

Dekang Zhu ◽

Xiaoyue Chen ◽

...

Keyword(s):

Bioinformatics Analysis ◽

Duck Enteritis Virus ◽

Glycoprotein G ◽

Enteritis Virus

Download Full-text

Bioinformatics Analysis of the Complete Nucleotide Sequence of UL17 Gene from Duck Enteritis Virus

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.647.227 ◽

2013 ◽

Vol 647 ◽

pp. 227-231

Author(s):

Ting Wen ◽

An Chun Cheng ◽

Ming Shu Wang

Keyword(s):

Nucleotide Sequence ◽

Bioinformatics Analysis ◽

Evolutionary Relationship ◽

Duck Enteritis Virus ◽

Fatal Disease ◽

Gallid Herpesvirus ◽

Genetic Vaccine ◽

Gallid Herpesvirus 2 ◽

Enteritis Virus ◽

Online Web

Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. Our laboratory had identified and sequenced the DEV-UL17 gene, one of the conservative genes of this viral genome. It is a 2055bp segment, GenBank accession NO. 643567. Here in this article we used kinds of bioinformatics softwares and online web servers to analyze the nucleotide sequence of this gene for further research. The phylogenetic tree of the DEV-UL17 protein and its homologs of other 19 reference herpesviruses revealed that DEV had close evolutionary relationship with Meleagrid herpesvirus1, Gallid herpesvirus 2 and Gallid herpesvirus 3, the members of genus Mardivirus. In addition, multiple epitopes and potential motifs were found in DEV-UL17 protein and they may play an important role in in the function of immune system, it may greatly influence the antibodies and genetic vaccine, or in the clinical diagnosis of DEV.

Download Full-text

Bioinformatics analysis and characteristics of VP23 encoded by the newly identified UL18 gene of duck enteritis virus

10.1117/12.913537 ◽

2011 ◽

Author(s):

Xiwen Chen ◽

Anchun Cheng ◽

Mingshu Wang ◽

Jun Xiang

Keyword(s):

Bioinformatics Analysis ◽

Duck Enteritis Virus ◽

Enteritis Virus

Download Full-text

Proteomic analysis of host cellular proteins co-immunoprecipitated with duck enteritis virus gC

Journal of Proteomics ◽

10.1016/j.jprot.2021.104281 ◽

2021 ◽

Vol 245 ◽

pp. 104281

Author(s):

Liu Chen ◽

Zheng Ni ◽

Jionggang Hua ◽

Weicheng Ye ◽

Keshu Liu ◽

...

Keyword(s):

Proteomic Analysis ◽

Duck Enteritis Virus ◽

Cellular Proteins ◽

Enteritis Virus

Download Full-text

Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Pathogens ◽

10.3390/pathogens10030365 ◽

2021 ◽

Vol 10 (3) ◽

pp. 365

Author(s):

Fengli Liu ◽

Yanxin Cao ◽

Maokai Yan ◽

Mengxu Sun ◽

Qingshui Zhang ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Colloidal Gold ◽

Preventive Intervention ◽

Virus Detection ◽

Cross Reactivity ◽

Duck Enteritis Virus ◽

Detection Sensitivity ◽

Immunochromatographic Assay ◽

Efficient Detection ◽

Enteritis Virus

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 103 TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.

Download Full-text