scholarly journals Detection of Edwardsiella tardain catfish (Clarias sp.)by fluorescent antibody technique (FAT)

2013 ◽  
Vol 11 (1) ◽  
pp. 96
Author(s):  
. Firma ◽  
Rahman Rizky Amalia ◽  
Utami Sari ◽  
Chotimah Chusbul ◽  
Abdulgani Amri Siregar
Keyword(s):  
Fluorescent Antibody ◽  
Culture Method ◽  
Infected Fish ◽  
Edwardsiella Tarda ◽  
Rabbit Serum ◽  
Fluorescent Antibody Technique ◽  
Fish Disease ◽  
Antibody Technique ◽  
Specificity Test ◽  
Kidney Liver

The fluorescent antibody technique (FAT) can be used to detectEdwardsiellatarda rapidly. As prelimenary step, it have been performed purity test of bacteria by PCR, specificity test of mouse anti-E. tarda monoclonal antibody by blocking using chicken and rabbit serum, and optimization of conjugate secondary antibody mouse IgG-H&amp;L (FITC) dillution rate. Fourty eights catfish were intraperitoneally injected by 0,3 mL of E. tarda with different concentration, namely: 102 CFU/mL, 103 CFU/mL, 105 CFU/mL. Kidney, spleen, and liver from three fishes in each treatment were collected at interval time of six hours, 12 hours, 24 hours, 48 hours after infection. The results showed that E. tarda could be detected in fish infected with 102 CFU of E. tarda after six hours of injection in kidney,liver, and spleen of infected fish. Hence, FAT is faster than detection by bacterial culture method, and this technique can be useful to prevent the spread of fish disease.<br /> <br />Keywords: fluorescent antibody technique, Edwardsiella tarda, detection, catfish<br /><br />

scholarly journals LOCALIZATION OF MYOGLOBIN IN HUMAN SKELETAL MUSCLE USING FLUORESCENT ANTIBODY TECHNIQUE

1967 ◽  
Vol 15 (8) ◽  
pp. 436-441 ◽  
Author(s):  
LAWRENCE J. KAGEN ◽  
RAYA GUREVICH
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Fluorescent Antibody ◽  
Intracellular Localization ◽  
Rabbit Antiserum ◽  
Rabbit Serum ◽  
Fluorescent Antibody Technique ◽  
Normal Rabbit Serum ◽  
Antibody Technique ◽  
Human Myoglobin

Rabbit antiserum to human myoglobin was used with the indirect fluorescent antibody technique to localize this protein in human skeletal muscle. Specific fluorescence was noted, in rapidly frozen and acetone-fixed sections, to be located at the transverse striations, at the sarcolemmal regions and at certain fibrillar structures within the cell. The antibody fluorescence reaction was shown to be specific for myoglobin, and was not produced by normal rabbit serum of serum of rabbits immunized with bacterial antigens. The reaction was abolished by prior absorption of the antimyoglobin serum with myoglobin, and was found to be absent in tissues deficient in myoglobin (lung, kidney, spleen, liver and uterus). Omission of acetone fixation or delayed freezing resulted in leakage of myoglobin from the cell and loss of specific intracellular localization. Sarcolemmal localization appeared to be somewhat more stable.

scholarly journals LOCALIZATION OF ADRENOCORTICOTROPIC HORMONE BY HISTOCHEMICAL AND IMMUNOCHEMICAL METHODS

1951 ◽  
Vol 94 (1) ◽  
pp. 21-30 ◽  
Author(s):  
John M. Marshall
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Fluorescent Antibody ◽  
Rabbit Serum ◽  
Fluorescent Antibody Technique ◽  
Normal Rabbit Serum ◽  
Protein Antigens ◽  
Antibody Technique ◽  
Selective Staining ◽  
Highly Active ◽  
Hog Kidney

The fluorescent antibody technique was adapted to the localization of native protein antigens in cells and tissues. This method was applied specifically to the localization of adrenocorticotropic hormone in the pituitary gland. An antiserum to hog ACTH was produced in an adrenalectomized rabbit. The γ2-globulin fraction of the serum was conjugated with fluorescein. After purification, the fluorescent antibody solution stained selectively the cytoplasm of basophil cells of the hog pituitary. No cells of sheep or beef pituitary or of hog kidney were stained. A fluorescent globulin solution prepared from normal rabbit serum gave no selective staining in any of these tissues. Immunochemical tests showed that the fluorescent antibody gave a precipitin reaction with a highly active ACTH preparation of low molecular weight. The supernatant solution from this reaction showed a loss of hormone activity.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

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