Localization of Coxsackie A16 Viral Antigen in Various Organs of Newborn Mice by the Fluorescent Antibody Technique

Brains of mice infected with western equine encephalitis (WEE) virus were fixed in Carnoy's fluid at 4 °C and transferred through chloroform to paraffin. The procedure gave excellent preservation of structural architecture and viral antigen. The latter was demonstrated in sections by the indirect fluorescent antibody technique in which Evans blue was used as a counterstain. The technique proved to be rapid, simple, and reliable for the identification of WEE virus in mouse brain.

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DETECTION OF VIREMIA OF JAPANESE ENCEPHALITIS IN THE NEW-BORN PIGLET AND SEARCH FOR VIRAL ANTIGEN IN ITS TISSUES BY MEANS OF FLUORESCENT ANTIBODY TECHNIQUE

Uirusu ◽

10.2222/jsv.18.317 ◽

1968 ◽

Vol 18 (4) ◽

pp. 317-322

Author(s):

Satoru OTSUKA ◽

Kenji MANAKO ◽

Masayoshi NASU ◽

Yuzo AOYAMA

Keyword(s):

Japanese Encephalitis ◽

Viral Antigen ◽

Fluorescent Antibody ◽

Fluorescent Antibody Technique ◽

Antibody Technique ◽

New Born

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Findings of bacterial microflora in piglets infected with conventional swine plague

Veterinarski glasnik ◽

10.2298/vetgl0204247p ◽

2002 ◽

Vol 56 (3-4) ◽

pp. 247-250

Author(s):

Jasna Prodanov ◽

Radoslav Dosen

Keyword(s):

Digestive Tract ◽

Bacterial Infections ◽

Viral Antigen ◽

Fluorescent Antibody ◽

Final Diagnosis ◽

Fluorescent Antibody Technique ◽

Antibody Technique

Piglets infected with the conventional swine plague virus as a result of secondary bacterial infections sometimes show an insufficiently clear clinical and pathoanatomical picture, which is why the very procedure of diagnosis is complex and the final diagnosis unreliable. That is why these investigations were aimed at examining the presence of bacterial microflora in diseased and dead pilgets which were found to have the viral antigen for CSP using the fluorescent antibody technique, in cases where the pathomorphological finding was not characteristic for conventional swine plague. Autopsies of dead piglets most often showed changes in the digestive tract and lungs, with resulting technopathy and diseases of infective nature. Such findings on knowledge of a present bacterial microflora are especially important in cases when conventional swine plague is controlled on farms and an announcement that the disease has been contained is in the offing.

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Demonstration of Infectious Bovine Rhinotracheitis Virus Antigen in Paraffin Sections

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063878900100202 ◽

1989 ◽

Vol 1 (2) ◽

pp. 105-109 ◽

Cited By ~ 8

Author(s):

Janice M. Miller ◽

Martin J. Van Der Maaten

Keyword(s):

Viral Antigen ◽

Virus Isolation ◽

Fluorescent Antibody ◽

Virus Antigen ◽

Fluorescent Antibody Technique ◽

Infectious Bovine Rhinotracheitis ◽

Paraffin Sections ◽

Antibody Technique ◽

Avidin Biotin Complex ◽

Infectious Bovine Rhinotracheitis Virus

Nine pregnant heifers were inoculated intravenously with infectious bovine rhinotracheitis virus (IBRV) in the sixth month of pregnancy. Tissues were collected from the fetus of a heifer killed 13 days postinoculation (PI), from fetuses of 6 heifers that aborted 16–27 days PI, and from mummified fetuses of 2 heifers that aborted 53 and 85 days PI, respectively. Control tissues were obtained from the fetus of a non-inoculated heifer that was killed in the seventh month of gestation. Tissues were fixed in 10% formalin, embedded in paraffin, and examined for viral antigen by immunohistochemistry, using biotinylated second antibody and alkaline phosphatase-labeled avidin-biotin complex. Antigen was detected in at least 1 tissue from the fetus of each inoculated heifer. Positive tissues included lung, liver, spleen, kidney, adrenal, and placenta. In several fetuses, antigen was identified in tissues from which virus was not isolated in cell culture. This appeared to occur when tissues had only a few small foci of infection or when tissues were severely autolyzed. The observation of viral antigen in tissues from mummified fetuses indicates that this technique may be useful in diagnostic laboratories to detect IBRV infection in tissues that are not suitable for virus isolation or for examination by the cryostat tissue section-fluorescent antibody technique.

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Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

Water Science & Technology ◽

10.2166/wst.2000.0459 ◽

2000 ◽

Vol 41 (4-5) ◽

pp. 301-308 ◽

Cited By ~ 1

Author(s):

N. Noda ◽

H. Ikuta ◽

Y. Ebie ◽

A. Hirata ◽

S. Tsuneda ◽

...

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Fluorescent Antibody ◽

Nitrifying Bacteria ◽

Fluorescent Antibody Technique ◽

Antibody Technique ◽

Antibody Method ◽

In Situ Detection ◽

Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

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Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽

10.1126/science.145.3635.943 ◽

1964 ◽

Vol 145 (3635) ◽

pp. 943-945 ◽

Cited By ~ 39

Author(s):

G. C. Brown ◽

H. F. Maassab ◽

J. A. Veronelli ◽

T. J. Francis

Keyword(s):

Human Serum ◽

Fluorescent Antibody ◽

Fluorescent Antibody Technique ◽

Indirect Fluorescent Antibody ◽

Antibody Technique ◽

Serum Detection

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LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

Journal of Histochemistry & Cytochemistry ◽

10.1177/13.3.155 ◽

1965 ◽

Vol 13 (3) ◽

pp. 155-160 ◽

Cited By ~ 16

Author(s):

H. J. KEUTEL

Keyword(s):

Kidney Stones ◽

Kidney Stone ◽

Fluorescent Antibody ◽

Human Kidney ◽

Fluorescent Antibody Technique ◽

Renal Tubules ◽

Antibody Technique ◽

The Matrix ◽

Normal Human ◽

The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

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