Observations on the growth and development of Bluetongue, Nairobi Sheep Disease and Rift Valley Fever viruses by fluorescent antibody technique and titration in a tissue culture system

1970 ◽  
Vol 29 (2-3) ◽  
pp. 127-138 ◽  
Author(s):  
H. Ohder ◽  
L. J. Lund ◽  
A. P. Whiteland
Keyword(s):  
Tissue Culture ◽  
Rift Valley ◽  
Growth And Development ◽  
Culture System ◽  
Rift Valley Fever ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Valley Fever ◽  
Antibody Technique ◽  
Tissue Culture System

scholarly journals An alphavirus replicon-derived candidate vaccine against Rift Valley fever virus

2009 ◽  
Vol 137 (9) ◽  
pp. 1309-1318 ◽  
Author(s):  
M. T. HEISE ◽  
A. WHITMORE ◽  
J. THOMPSON ◽  
M. PARSONS ◽  
A. A. GROBBELAAR ◽  
...  
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fluorescent Antibody ◽  
Severe Disease ◽  
Neutralizing Antibody ◽  
Fever Virus ◽  
Antibody Responses ◽  
Valley Fever

SUMMARYRift Valley fever virus (RVFV) is a mosquito-transmitted bunyavirus (genusPhlebovirus) associated with severe disease in livestock and fatal encephalitis or haemorrhagic fever in a proportion of infected humans. Although live attenuated and inactivated vaccines have been used in livestock, and on a limited scale in humans, there is a need for improved anti-RVFV vaccines. Towards this goal, Sindbis virus replicon vectors expressing the RVFV Gn and Gc glycoproteins, as well as the non-structural nsM protein, were constructed and evaluated for their ability to induce protective immune responses against RVFV. These replicon vectors were shown to produce the RVFV glycoproteins to high levelsin vitroand to induce systemic anti-RVFV antibody responses in immunized mice, as determined by RVFV-specific ELISA, fluorescent antibody tests, and demonstration of a neutralizing antibody response. Replicon vaccination also provided 100% protection against lethal RVFV challenge by either the intraperitoneal or intranasal route. Furthermore, preliminary results indicate that the replicon vectors elicit RVFV-specific neutralizing antibody responses in vaccinated sheep. These results suggest that alphavirus-based replicon vectors can induce protective immunity against RVFV, and that this approach merits further investigation into its potential utility as a RVFV vaccine.

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