In vitro, Alternative Methods to the Biological Control of the Aphids by Entomopathogenic Fungi Beauveria bassiana Isolates from Gaza Strip

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

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Biological control of white grubs, Holotrichia serrata (Fabricius) in sugarcane by two species of entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana

International Journal of Tropical Insect Science ◽

10.1007/s42690-020-00256-y ◽

2020 ◽

Author(s):

B. Ramanujam ◽

A.C. Hosamani ◽

B. Poornesha ◽

E. Sowmya

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

White Grubs ◽

Holotrichia Serrata

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In Vitro Inhibition of the Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae by Six Fungicides Used in Pecan Culture 1

Environmental Entomology ◽

10.1093/ee/10.3.346 ◽

1981 ◽

Vol 10 (3) ◽

pp. 346-349 ◽

Cited By ~ 22

Author(s):

W. L. Tedders

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

In Vitro Inhibition

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Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d190647 ◽

2018 ◽

Vol 19 (6) ◽

pp. 2365-2373 ◽

Cited By ~ 1

Author(s):

AYU SAFITRI ◽

SITI HERLINDA ◽

ARUM SETIAWAN

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Insect Pests ◽

Vegetation Type ◽

Tenebrio Molitor ◽

Soil Samples ◽

Fungal Isolates

Safitri A, Herlinda S, Setiawan A. 2018. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas 19: 2365-2373. Ecosystems of lowlands and highlands in South Sumatra have specific characteristics of soils and vegetations that can affect the availability of entomopathogenic fungi. This study aimed to explore and identify species and to determine inoculum potentials of the entomopathogenic fungi from soils of freshwater swamps, tidal lowlands, peatlands, and highlands. Baiting of entomopathogenic fungi on soil samples used the larvae of Tenebrio molitor. The entomopathogenic fungi species found in this research were Beauveria bassiana and Metarhizium anisopliae. The number of the fungal isolates were 30 isolates consisting of nine isolates of B. bassiana and 21 isolates of M. anisopliae.The highest number of isolates was found in the highland ecosystem (11 isolates) and the lowest was found in peatland ecosystem (4 isolates). The highest percentage average of inoculum potentials of the fungi was found in the high land ecosystem (4.04%) and the lowest one was found in freshwater swamps ecosystem (2.11%). Based on the vegetation type, the soil planted with mustard in Talang Patai-Pagaralam (highland ecosystem) had the highest inoculum potentials (9.33%). These fungi will make an important contribution to the biological control for insect pests in lowland to highland ecosystems in Indonesia.

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Effect of Agrochemicals Used in the Cultivation of Soybean and Irrigated Rice on Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

Journal of Agricultural Science ◽

10.5539/jas.v11n17p167 ◽

2019 ◽

Vol 11 (17) ◽

pp. 167

Author(s):

Vanessa P. Gonçalves ◽

Cândida R. J. de Farias ◽

Victoria Moreira-Nunêz ◽

Renata Moccellin ◽

Viviana Gaviria-Hernández ◽

...

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Spore Germination ◽

Metarhizium Anisopliae ◽

Mycelial Growth ◽

Potato Dextrose Agar ◽

Irrigated Rice ◽

Spray Method ◽

Fungitoxic Effect

The objective of this work was to evaluate the fungitoxic effect of the agrochemicals used in the cultivation of soybean and irrigated rice on entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae by means of the mycelial growth, sporulation and spore germination. The isolates were inoculated in potato-dextrose-agar (PDA) medium containing the pesticides and exposed to spraying with the products. It was observed that chlorantraniliprole produced the best results regarding and was compatible with the two methods of contact of the product with the fungi. Flubendiamide it’s very toxic to fungi, producing 100% inhibition when incorporated into the medium, when by spraying, the fungus M. anisopliae got mycelial growth. Etofenprox and thiamethoxam changed their toxity classification according to the method of contact with product, was moderately compatible to B. bassiana and compatible to M. anisopliae and spinosad showed more compatibility with M. anisopliae than B. bassiana and was classified as compatible. Among fungicides the tricyclazole was the only compatible with the fungus by the spray method. The results showed that in vitro agrochemicals such as tricyclazole, thiamethoxam, flubendiamide and etofenprox are harmful to fungi. It was found that environmental interference could minimize the effects on organisms, especially when the chemicals are applied by spraying.

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Test of Various Carrier Materials Against Viability and Conidia Density in Some Liquid Biopesticides of Entomopathogenic Fungi

JURNAL AGRONIDA ◽

10.30997/jag.v5i1.1851 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Unun Triasih ◽

Dina Agustina ◽

Mutia Erti Dwiastuti ◽

...

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Biological Diversity ◽

Initial Density ◽

Plant Diseases ◽

Carrier Material ◽

Carrier Materials ◽

One Year ◽

Beauveria Bassiana Isolates

Indonesia is a country that has a large biological diversity but has not been fully utilized for agriculture. The effectiveness of biological agents in controlling pests and plant diseases still needs further investigation, one of which is the use of entomopathogenic fungi as biopesticides. The purpose of this study was to determine the effect of storage on the viability and density of conidia in several entomopathogenic fungi; Beauveria bassiana, Metarhizium anisopliae, Paecilomyces. This study used 5 entomopathogenic fungi isolates, 2 Beauveria bassiana isolates (JBG and JBR), 2 Metarhizium anisopliae isolates (JBG and UST), 1 Paecilomyces. Biopesticide carrier material consisted of 6 carrier materials, namely soybean decoction waste, soy washing waste, Potato Dextrose Agar, tapioca waste, rice waste, rice washing waste and coconut water. Biopesticides have been made one year before and the viability and complexity of the conidia is re-examined one year later. From the observations of conidial density, the best was found in Beauveria bassiana JBG liquid biopesticide of 4.8 x 10 x conidia / ml from the initial density of 3.4 x 10⁸ / ml in the soybean decoction (RK) carrier material. The highest viability was found in JBG Metharizium anisopliae on soybean washing waste material by 14%. Key words: Conidia viability, conidial density, entomopathogenic fungi

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Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle

Journal of Insect Science ◽

10.1093/jisesa/iev029 ◽

2015 ◽

Vol 15 (1) ◽

pp. 44-44 ◽

Cited By ~ 34

Author(s):

F. Erler ◽

A. O. Ates

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Biological Control Agents

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Isolation of Beauveria Strains and Their Potential as Control Agents for Lema bilineata Germar (Coleoptera: Chrysomelidae)

Insects ◽

10.3390/insects13010093 ◽

2022 ◽

Vol 13 (1) ◽

pp. 93

Author(s):

Jason Lee Furuie ◽

Andressa Katiski da Costa Stuart ◽

Morgana Ferreira Voidaleski ◽

Maria Aparecida Cassilha Zawadneak ◽

Ida Chapaval Pimentel

Keyword(s):

Molecular Markers ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Biocontrol Agent ◽

Physalis Peruviana ◽

Solanaceae Species ◽

Ovicidal Effect ◽

Ovicidal Effects ◽

Commercial Field

Lema bilineata Germar (Coleoptera: Chrysomelidae) was recently reported to damage Physalis peruviana crops in Brazil. Given the potential for inflicting damage on other Solanaceae species and the lack of alternatives for controlling this pest, we assessed the pathogenicity of 15 Beauveria isolates against L. bilineata adults in vitro. In addition, three of these isolates were tested for their ovicidal effect against L. bilineata eggs. Fungal strains were isolated from mummified corpses of L. bilineata collected in a non-commercial field in Paraná, Brazil. The isolates were identified as Beauveria bassiana using molecular markers. Lema bilineata adults were susceptible to conidial suspensions of all these isolates at a concentration of 108 conidia mL−1. Deaths caused by fungal extrusion were confirmed. Three strains were found to be more virulent against L. bilineata adults and showed ovicidal effects. This is the first study on entomopathogenic fungi isolated from dead insects collected from P. peruviana crops and tested against L. bilineata carried out in Brazil. The results obtained in the laboratory indicate the high potential of the use of three B. bassiana strains against L. bilineata as a biocontrol agent.

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