scholarly journals Prevalence and Detection of Tomato Leaf Curl Virus from Low Altitude Subtropical Areas of Jammu and Kashmir

2016 ◽  
Vol 5 (11) ◽  
pp. 768-773
Author(s):  
Ranbir Singh ◽  
V.K. Razdan ◽  
Manika Sharma
Keyword(s):  
Tomato Leaf ◽  
Jammu And Kashmir ◽  
Tomato Leaf Curl Virus ◽  
Low Altitude ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Use of Tomato leaf curl virus (TYLCV) truncated Rep gene sequence to engineer TYLCV resistance in tomato plants

2009 ◽  
Vol 53 (2) ◽  
pp. 99-104 ◽  
Author(s):  
H. Tamarzizt ◽  
S. Chouchane ◽  
R. Lengliz ◽  
D. Maxwell ◽  
M. Marrakchi ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Tomato Leaf ◽  
Tomato Plants ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Evidence for recombination among the tomato leaf curl virus strains/species from Bangalore, India

2002 ◽  
Vol 147 (2) ◽  
pp. 255-272 ◽  
Author(s):  
N. Kirthi ◽  
S. P. Maiya ◽  
M. R. N. Murthy ◽  
H. S. Savithri
Keyword(s):  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Virus Strains ◽  
Leaf Curl

scholarly journals Molecular Characterization of a New Tomato Begomovirus from India

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 598-598 ◽  
Author(s):  
S. L. Shih ◽  
W. S. Tsai ◽  
S. K. Green ◽  
P. M. Hanson ◽  
G. B. Valand ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Western India ◽  
Breeding Lines ◽  
Tomato Leaf Curl Virus ◽  
Northern India ◽  
Tomato Breeding ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Virus Isolates ◽  
Leaf Curl

The Asian Vegetable Research and Development Center's (AVRDC) tomato breeding lines derived from Lycopersicon hirsutum f. glabratum B 6013 × L. esculentum H-24 and carrying the Ty-2 resistance gene located on chromosome 11 are tolerant to tomato leaf curl disease in Karnataka State, southern India (3), where several isolates of Tomato leaf curl Virus-Bangalore (GenBank Accession Nos. L11746, Z48182, and AF165098) and Tomato leaf curl virus-Karnataka (GenBank Accession No. U38239) are reported to infect tomatoes. The only area in south and southeast Asia where these AVRDC tomato breeding lines were found susceptible to begomovirus infection is Thailand, where several bipartite Tomato yellow leaf curl virus isolates (GenBank Accession Nos. X63015, X63016; AF141922, AF141897; and AF511529, AF511528) are reported to be prevalent. However, in field trials conducted in the fall of 1999 in Bodeli, Gujarat State, western India, the AVRDC breeding lines showed typical symptoms of begomovirus infection, such as leaf curling and vein clearing. The presence of a different tomato begomovirus was suspected. Viral DNA from a symptomatic plant from Bodeli was amplified by polymerase chain reaction (PCR) using the begomovirus-specific degenerate primer pair PAL1v1978/PAR1c715 (4) and the expected 1.4-kb PCR product was obtained. Based on the sequence of the 1.4-kb DNA product, specific primers were designed to complete the DNA-A sequence. The DNA-A of the virus associated with tomato leaf curl from Bodeli consists of 2,759 nucleotides (GenBank Accession No. AF413671) and contains six open reading frames (ORFs V1, V2, C1, C2, C3, and C4). The DNA-A sequence of the Bodeli isolate had highest sequence identities of 98 and 98.3%, respectively, with viruses causing tomato leaf curl from Varanasi, Uttar Pradesh State, northern India (GenBank Accession No. AF449999) collected in the fall of 1999 and Panchkhal, Nepal (GenBank Accession No. AY234383) collected in early 2000. There was no evidence for the presence of DNA-B in the Bodeli, Panchkhal, or Varanasi virus isolates using DNA-B specific primer pairs DNABLC1/DNABLV2 and DNABLC2/DNABLV2 (2). However, a 1.3-kb DNA-beta was detected in the Panchkhal and Varanasi isolates using the primer pair Beta01/Beta02 (1). Sequence comparisons with begomovirus sequences available in the GenBank database showed that these three virus isolates and GenBank Accession No. AY190290 collected in 2001 from Varanasi shared more than 97% sequence identity with each other and should be considered closely related strains of the same virus. These four virus isolates belong to a new distinct tomato geminivirus species because their sequences share less than 88% sequence identities with the next most closely related virus, Tomato leaf curl virus-Karnataka (GenBank Accession No. U38239). This new tomato leaf curl virus is prevalent in western India, northern India, and Nepal. References: (1) R. W. Briddon et al. Mol. Biotechnol. 20:315, 2002. (2) S. K. Green et al. Plant Dis. 85:1286, 2001. (3) V. Muniyappa et al. HortScience 37:603, 2002. (4) M. R. Rojas et al. Plant Dis. 77:340, 1993.

scholarly journals Effects of Tomato Leaf Curl Virus on Growth and Yield Parameters of Tomato Crop

2021 ◽  
Vol 39 (1) ◽  
pp. 79-83
Author(s):  
Yasir Iftikhar ◽  
◽  
Mustansar Mubeen ◽  
Ashara Sajid ◽  
Mohamed Ahmad Zeshan ◽  
...  
Keyword(s):  
Disease Severity ◽  
Disease Incidence ◽  
Tomato Leaf ◽  
Growth And Yield ◽  
Tomato Crop ◽  
Tomato Leaf Curl Virus ◽  
Yield Parameters ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Iftikhar, Y., M. Mubeen, A. Sajid, M.A. Zeshan, Q. Shakeel, A. Abbas, S. Bashir, M. Kamran and H. Anwaar. 2021. Effects of Tomato Leaf Curl Virus on Growth and Yield Parameters of Tomato Crop. Arab Journal of Plant Protection, 39(1): 79-83. Tomato is an important vegetable crop, belongs to the family Solanaceae and is the second most consumed vegetable following potatoes. The tomato crop is grown all over the world in both summer and winter seasons, and plant viruses are a major threat to tomato production. Among these viruses, tomato leaf curl virus (TLCV) causes considerable yield loss to tomato crop. This virus is transmitted by a whitefly (Bemisia tabaci) vector. In this study, the effect of TLCV infection, on the following tomato growth and yield parameters, was evaluated: plant leaf number and area, plant biomass, plant height, root length, and plant stem diameter and yield. Tomato plants were transplanted in wellprepared plots with 4 replications. The control group was covered with polyethene bag to avoid whitefly infestation. Plants were scored on the 15th and 30th day after inoculation and TLCV disease severity was recorded. Analysis of variance (ANOVA) showed the significant differences between the healthy and infected tomato plants. Moreover, growth and yield parameters were reduced with the increase in disease incidence, disease severity and whitefly infestation. Disease severity was increased with the increase in temperature during the growing season. It can be concluded from this study that TLCV significantly affects growth and yield of the tomato crop. Keywords: Tomato, Tomato leaf curl virus, TLCV, disease incidence, disease severity.

scholarly journals EFFECT OF ENVIRONMENTAL FACTORS ON BIOCHEMICAL PROPERTIES OF TOMATO LEAF CURL VIRUS INFECTED LEAVES OF TOMATO

2019 ◽  
Vol 31 (1) ◽  
pp. 105-111
Author(s):  
Saneela Arooj ◽  
Yasir Iftekhar ◽  
Mustansar Mubeen ◽  
Muhammad I. Ullah ◽  
Ashara Sajid ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Biochemical Properties ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection

2019 ◽  
Vol 14 (3) ◽  
pp. e1565595 ◽  
Author(s):  
Ravindra K. Chandan ◽  
Achuit K. Singh ◽  
Sunita Patel ◽  
Durga Madhab Swain ◽  
Narendra Tuteja ◽  
...  
Keyword(s):  
Virus Infection ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Analysis of Silencing Escape of Tomato leaf curl virus: An Evaluation of the Role of DNA Methylation

2006 ◽  
Vol 19 (6) ◽  
pp. 614-624 ◽  
Author(s):  
Xue-Yu Bian ◽  
M. Saif Rasheed ◽  
Mark J. Seemanpillai ◽  
M. Ali Rezaian
Keyword(s):  
Transgenic Plants ◽  
Rna Silencing ◽  
De Novo ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Sense Strand ◽  
High Level ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

RNA silencing is a sequence-specific mechanism regulating gene expression and has been used successfully for antiviral defense against RNA viruses. Similar strategies to develop resistance against DNA containing Tomato leaf curl virus (TLCV) and some other geminiviruses have been unsuccessful. To analyze this silencing escape, we transformed tomato plants with a hairpin construct from the TLCV C2 open reading frame (ORF). The transgenic plants showed a strong RNA silencing response, and following TLCV inoculation, their infection was delayed. However, the viral infection was not prevented and TLCV DNA accumulated to the levels found in nontransgenic plants. To determine the fate of a transgene carrying homology to the virus, we used transgenic plants carrying the TLCV C4 gene, which induces a distinct phenotype. Upon TLCV infection, the phenotype was abolished and C4 transcript disappeared. Concurrently, TLCV-specific small interfering RNAs were produced. In situ hybridization showed abundant levels of TLCV DNA in phloem cells of TLCV-infected C4 trans-genic plants. However, the C4 transcripts were no longer detectable in nonvascular cells. Analysis of the transgene by methylation sequencing revealed a high level of de novo methylation of asymmetric cytosines in both the C4 ORF and its 35S promoter. A high level of methylation also was found at both symmetric and asymmetric cytosines of the complementary-sense strand of TLCV double-stranded DNA. Given the previous finding that methylated geminivi-ral DNA is not competent for replication, we provide a model whereby TLCV evades host defense through a population of de novo synthesized unmethylated DNA.

Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum

2009 ◽  
Vol 119 (3) ◽  
pp. 519-530 ◽  
Author(s):  
Ilana Anbinder ◽  
Moshe Reuveni ◽  
Raviv Azari ◽  
Ilan Paran ◽  
Sahadia Nahon ◽  
...  
Keyword(s):  
Virus Resistance ◽  
Tomato Leaf ◽  
Molecular Dissection ◽  
Tomato Leaf Curl Virus ◽  
Tomato Line ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl
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