In vitro neoformation of grape chimeras (Vitis vinifera L.)
<p style="text-align: justify;">Difference in grape sensitivity to <em>Botrytis cinerea</em> attacks between cultivars was explained by differences in the epidermic tissue of the fruit. Therefore, this work was conducted to create a grape periclinal chimera, whose fruits would combine the skin of a <em>Botrytis</em> tolerant cultivar with a pulp of an another cultivar admitted its good organoleptic quality and productivity. In a first time, graftings of two cultivars (Chardonnay and Pinot noir) were conducted <em>in vitro</em> on 5 different media supplemented with various plant growth regulators. Adventitious shoots were only observed on medium containing BAP and GA3 from a mixed callus structure after four weeks of darkness followed by a light/dark regime. In a second time, RAPD analysis, conducted on these plants, showed their chimerical characteristics.</p>