scholarly journals Sanitary status of slovenian indigenous grapevine cultivar Refosk

OENO One ◽  
2005 ◽  
Vol 39 (1) ◽  
pp. 19 ◽  
Author(s):  
Irma Tomazic ◽  
Zora Korosec-Koruza ◽  
Natasa Petrovic
Keyword(s):  
Vitis Vinifera ◽  
Mosaic Virus ◽  
Vitis Vinifera L ◽  
Grapevine Virus ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Disease Symptoms ◽  
Grapevine Cultivar ◽  
Grapevine Virus A ◽  
Grapevine Virus B

<p style="text-align: justify;">Sanitary status of visually selected and nonselected indigenous grapevine cultivar 'Refosk' (Vitis vinifera L. cv. 'Refosk') was evaluated. Nine viruses were tested by ELISA: Nepoviruses Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), Closteroviruses Grapevine leafroll associated viruses 1 (GLRaV-1), 2 (GLRaV-2), 3 (GLRaV-3), and 6 (GLRaV-6), Grapevine fleck virus (GFkV) and Vitiviruses Grapevine virus A (GVA) and Grapevine virus B (GVB). All viruses except ArMV and GVB were detected. The vines of cv. ‘Refosk’ passing the visual selection were 50 % free of tested viruses in comparison to only 24 % virus-free vines of non-visually selected material. Visually selected plants revealed the highest incidence of virus GLRaV- 1 (37 %) and in addition to that a 15 % incidence of rugose wood (RW) disease symptoms.</p>

scholarly journals Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas

2010 ◽  
Vol 40 (11) ◽  
pp. 2249-2255 ◽  
Author(s):  
Marcos Fernando Basso ◽  
Thor Vinícius Martins Fajardo ◽  
Marcelo Eiras ◽  
Ricardo Antônio Ayub ◽  
Osmar Nickel
Keyword(s):  
Mosaic Virus ◽  
Grapevine Virus ◽  
Rt Pcr ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Para Determinar ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

A propagação vegetativa da videira favorece infecções virais múltiplas, com expressão diferencial de sintomas em função da combinação da cultivar ou espécie da hospedeira com a espécie viral. Os objetivos deste trabalho foram detectar e identificar as espécies virais presentes em duas espécies/cultivares de videira: uma sintomática e outra assintomática. DsRNA de ambas as amostras foi submetido à RT-PCR com 17 pares de oligonucleotídeos específicos para a detecção de Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine fleck virus (GFkV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine chrome mosaic virus (GCMV), Rupestris stem pitting-associated virus (RSPaV), Grapevine leafroll-associated virus 1 a 4 (GLRaV-1 a -4), além de três pares de oligonucleotídeos degenerados. Pelo menos um fragmento amplificado, por par de oligonucleotídeos, foi clonado e sequenciado. Plantas sintomáticas e assintomáticas mostraram infecções múltiplas por RSPaV, GLRaV-2 e/ou GLRaV-3. As sequências de nucleotídeos obtidas para sete isolados de RSPaV, três de GLRaV-2 e dois de GLRaV-3 apresentaram identidades superiores a 90% com espécies homólogas e permitiram a definição das possíveis estirpes presentes nas amostras infectadas. Esses resultados evidenciam a necessidade da diagnose viral baseada em testes específicos para determinar a condição sanitária da videira.

scholarly journals Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais

2015 ◽  
Vol 45 (3) ◽  
pp. 379-385 ◽  
Author(s):  
Aricléia de Moraes Catarino ◽  
Thor Vinícius Martins Fajardo ◽  
Gilvan Pio-Ribeiro ◽  
Marcelo Eiras ◽  
Osmar Nickel
Keyword(s):  
Grapevine Virus ◽  
Rt Pcr ◽  
Grapevine Fanleaf Virus ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

Os objetivos deste trabalho foram identificar as espécies virais presentes em vinhedos comerciais de duas regiões do Nordeste do Brasil e realizar a caracterização molecular parcial de isolados de três espécies virais. A diagnose foi realizada por meio de RT-PCR em tempo real para a detecção de Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine leafroll-associated virus 2, 3 e 4 (GLRaV-2, -3 e -4), Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV) e Grapevine fanleaf virus (GFLV). Exceto para GFLV, os vírus avaliados estão amplamente disseminados nas áreas amostradas, frequentemente em altas incidências e em infecções múltiplas, de até 98% e 76,4%, na Zona da Mata e no Vale do São Francisco, respectivamente. Isolados locais de GVA, GVB e GLRaV-3 foram parcialmente caracterizados com base na sequência completa de nucleotídeos do gene da proteína capsidial e apresentaram alta porcentagem de identidade de nucleotídeos com outros isolados brasileiros: 91,2% (GVA), 99,8% (GVB) e 99,7% (GLRaV-3)

Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L

Plant Science ◽  
2003 ◽  
Vol 165 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Qiaochun Wang ◽  
Munir Mawassi ◽  
Ping Li ◽  
Ron Gafny ◽  
Ilan Sela ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Shoot Tips ◽  
Vitis Vinifera L ◽  
Grapevine Virus ◽  
Grapevine Virus A

scholarly journals Evaluation of sanitary status of grapevines in the Czech Republic

2011 ◽  
Vol 49 (No. 2) ◽  
pp. 63-66 ◽  
Author(s):  
P. Komínek ◽  
V. Holleinová
Keyword(s):  
Czech Republic ◽  
Grapevine Virus ◽  
Arabis Mosaic Virus ◽  
The Czech Republic ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Near Future ◽  
Infected Grapevine ◽  
Das Elisa ◽  
Propagation Material

A survey was made to evaluate sanitary status of grapevines in the Czech Republic with regard to occurrence of economically important viruses. Propagation material of 109 grapevine clones was tested for presence of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine leafroll-associated virus 1, Grapevine leafroll-associated virus 3, Grapevine virus A, Grapevine virus B and Grapevine fleck virus. Dormant canes were collected and cortical scrappings were analyzed by DAS-ELISA. All seven viruses tested were found to be widely spread in Czech propagation material of grapevine. From 330 individual vines tested, 148 vines were found to be infected with at least one virus. From 109 clones tested, in 98 clones at least one vine negative for tested pathogens was found. Such vines were promoted as candidate plants into screenhouse in Faculty of Horticulture Lednice and will be further tested by other methods. Sanitation of infected grapevine clones is needed in near future.

scholarly journals The detection of viruses and phytoplasmas in dwarfed shoots of grapevine varieties Aurelius and Neuburger

2012 ◽  
Vol 60 (8) ◽  
pp. 73-78
Author(s):  
Věra Holleinová ◽  
J. Čechová
Keyword(s):  
Mixed Infection ◽  
The Other ◽  
Grapevine Virus ◽  
Grapevine Fanleaf Virus ◽  
Short Shoot ◽  
Arabis Mosaic Virus ◽  
Molecular Tests ◽  
Grapevine Virus A ◽  
Phytoplasma Infection ◽  
Stolbur Phytoplasma

The survey of occurrence of six chosen virus pathogens and phytoplasmas complex was done on plants of grapevine (Vitis vinifera L.) which showed symptoms of short-shoot syndrom. The results of serological and molecular tests did not confirm either virus or phytoplasma infection as the main source of short-shoot syndrom. The presence of 6 viruses in samples taken from 45 affected plants of grapevine on 4 habitats. The highest occurrence of viruses was found out on habitat Moravská Nová Ves, where all taken samples were infected by Grapevine leafroll-associated virus GLRaV-1 (100 %). In 66 % of the samples taken from that habitat were detected mixed infection of Grapevine virus A and GLRaV-1. These 2 pathogens but were not detected in samples from affected plants from the other habitats or very sporadically – just in 2 plants. Another 4 virus pathogens were detected either sporadically (Grapevine fleck virus, Grapevine leafroll-assoc. virus GLRaV-3, Arabis mosaic virus) or not at all (Grapevine fanleaf virus). From 270 tests made to 6 viruses were only 20 positive, e. g. 7.4 %. It means that from 45 plants were 15 infected at least by 1 virus (33 %). The phytoplasmas complex was tested in 28 plants. The result was positive only in 1 plant, by another test the Potato stolbur phytoplasma was confirmed.

scholarly journals Dissecting interplays between Vitis vinifera L. and grapevine virus B (GVB) under field conditions

2018 ◽  
Vol 19 (12) ◽  
pp. 2651-2666 ◽  
Author(s):  
Walter Chitarra ◽  
Danila Cuozzo ◽  
Alessandra Ferrandino ◽  
Francesca Secchi ◽  
Sabrina Palmano ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Field Conditions ◽  
Vitis Vinifera L ◽  
Grapevine Virus ◽  
Grapevine Virus B

Prevalence and genetic diversity of viruses associated with rugose wood complex in Greek vineyards

Plant Disease ◽  
2021 ◽  
Author(s):  
Chrysoula Orfanidou ◽  
Kalliopi Moraki ◽  
Polina Panailidou ◽  
Leonidas Lotos ◽  
Asimina T Katsiani ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
First Record ◽  
Grapevine Virus ◽  
Multiple Introductions ◽  
Worldwide Distribution ◽  
Grapevine Virus A ◽  
Transmission Modes ◽  
Virus Incidence ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting

Rugose wood is one of the most important disease syndromes of grapevine and it has been associated with at least three viruses: grapevine rupestris stem pitting associated virus (GRSPaV), grapevine virus A (GVA) and grapevine virus B (GVB). All three viruses show a worldwide distribution pattern, and their genetic composition has been the focus of extensive research over the past years. Despite their first record in Greece almost 20 years ago, there is a lack of knowledge on the distribution and genetic variability of their populations in Greek vineyards. In this context, we investigated the distribution of GRSPaV, GVA and GVB in rootstocks, self-rooted and grafted grapevine cultivars, originating from different geographic regions that are representing important viticultural areas of Greece. Three new RT-PCR assays were developed for the reliable detection of GRSPaV, GVA and GVB. Our results indicated that GVA is the most prevalent in Greek vineyards, followed by GRSPaV and GVB. However, virus incidence differed among self-rooted and grafted grapevine cultivars or rootstocks tested. Selected isolates from each virus were further molecularly characterized to determine their phylogenetic relationships. All three viruses exhibited high nucleotide diversity, which was depicted in the constructed phylogenetic trees. Isolates from Greece were placed in various phylogroups, reinforcing the scenario of multiple introductions of GVA, GVB and GRSPaV in Greece and highlighting the effect of different transmission modes in the evolutionary course of the three viruses.

Sign in / Sign up

Export Citation Format

Share Document