scholarly journals Evaluation of sanitary status of grapevines in the Czech Republic

2011 ◽  
Vol 49 (No. 2) ◽  
pp. 63-66 ◽  
Author(s):  
P. Komínek ◽  
V. Holleinová
Keyword(s):  
Czech Republic ◽  
Grapevine Virus ◽  
Arabis Mosaic Virus ◽  
The Czech Republic ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Near Future ◽  
Infected Grapevine ◽  
Das Elisa ◽  
Propagation Material

A survey was made to evaluate sanitary status of grapevines in the Czech Republic with regard to occurrence of economically important viruses. Propagation material of 109 grapevine clones was tested for presence of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine leafroll-associated virus 1, Grapevine leafroll-associated virus 3, Grapevine virus A, Grapevine virus B and Grapevine fleck virus. Dormant canes were collected and cortical scrappings were analyzed by DAS-ELISA. All seven viruses tested were found to be widely spread in Czech propagation material of grapevine. From 330 individual vines tested, 148 vines were found to be infected with at least one virus. From 109 clones tested, in 98 clones at least one vine negative for tested pathogens was found. Such vines were promoted as candidate plants into screenhouse in Faculty of Horticulture Lednice and will be further tested by other methods. Sanitation of infected grapevine clones is needed in near future.

scholarly journals Genetic and biological characterisation of a Grapevine virus A isolate from the Czech Republic

2009 ◽  
Vol 44 (No. 4) ◽  
pp. 121-126
Author(s):  
P. Komínek ◽  
M. Komínková
Keyword(s):  
Czech Republic ◽  
Coat Protein ◽  
Genomic Sequence ◽  
Movement Protein ◽  
Amino Acid Identity ◽  
Grapevine Virus ◽  
The Czech Republic ◽  
Grapevine Cultivar ◽  
Grapevine Virus A ◽  
Kober Stem Grooving

An isolate of <i>Grapevine virus A</i> (GVA) from the Czech Republic was obtained from the grapevine cultivar Müller Thurgau. Symptoms of GVA – Kober stem grooving disease were not observed in the infected grapevines (which had been grafted onto Kober 5BB rootstock). A partial genomic sequence of the GVA isolate, 1523 nucleotides long, was obtained. The sequence completely covers the genes for both a movement and a coat protein. Compared to the GVA sequences available in databases, the nucleotide identity reached 84%. The amino acid identity in the movement protein reached 88%, and 98% in the coat protein.

scholarly journals Sanitary status of slovenian indigenous grapevine cultivar Refosk

OENO One ◽  
2005 ◽  
Vol 39 (1) ◽  
pp. 19 ◽  
Author(s):  
Irma Tomazic ◽  
Zora Korosec-Koruza ◽  
Natasa Petrovic
Keyword(s):  
Vitis Vinifera ◽  
Mosaic Virus ◽  
Vitis Vinifera L ◽  
Grapevine Virus ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Disease Symptoms ◽  
Grapevine Cultivar ◽  
Grapevine Virus A ◽  
Grapevine Virus B

<p style="text-align: justify;">Sanitary status of visually selected and nonselected indigenous grapevine cultivar 'Refosk' (Vitis vinifera L. cv. 'Refosk') was evaluated. Nine viruses were tested by ELISA: Nepoviruses Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), Closteroviruses Grapevine leafroll associated viruses 1 (GLRaV-1), 2 (GLRaV-2), 3 (GLRaV-3), and 6 (GLRaV-6), Grapevine fleck virus (GFkV) and Vitiviruses Grapevine virus A (GVA) and Grapevine virus B (GVB). All viruses except ArMV and GVB were detected. The vines of cv. ‘Refosk’ passing the visual selection were 50 % free of tested viruses in comparison to only 24 % virus-free vines of non-visually selected material. Visually selected plants revealed the highest incidence of virus GLRaV- 1 (37 %) and in addition to that a 15 % incidence of rugose wood (RW) disease symptoms.</p>

scholarly journals Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas

2010 ◽  
Vol 40 (11) ◽  
pp. 2249-2255 ◽  
Author(s):  
Marcos Fernando Basso ◽  
Thor Vinícius Martins Fajardo ◽  
Marcelo Eiras ◽  
Ricardo Antônio Ayub ◽  
Osmar Nickel
Keyword(s):  
Mosaic Virus ◽  
Grapevine Virus ◽  
Rt Pcr ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Para Determinar ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

A propagação vegetativa da videira favorece infecções virais múltiplas, com expressão diferencial de sintomas em função da combinação da cultivar ou espécie da hospedeira com a espécie viral. Os objetivos deste trabalho foram detectar e identificar as espécies virais presentes em duas espécies/cultivares de videira: uma sintomática e outra assintomática. DsRNA de ambas as amostras foi submetido à RT-PCR com 17 pares de oligonucleotídeos específicos para a detecção de Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine fleck virus (GFkV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine chrome mosaic virus (GCMV), Rupestris stem pitting-associated virus (RSPaV), Grapevine leafroll-associated virus 1 a 4 (GLRaV-1 a -4), além de três pares de oligonucleotídeos degenerados. Pelo menos um fragmento amplificado, por par de oligonucleotídeos, foi clonado e sequenciado. Plantas sintomáticas e assintomáticas mostraram infecções múltiplas por RSPaV, GLRaV-2 e/ou GLRaV-3. As sequências de nucleotídeos obtidas para sete isolados de RSPaV, três de GLRaV-2 e dois de GLRaV-3 apresentaram identidades superiores a 90% com espécies homólogas e permitiram a definição das possíveis estirpes presentes nas amostras infectadas. Esses resultados evidenciam a necessidade da diagnose viral baseada em testes específicos para determinar a condição sanitária da videira.

scholarly journals Strategic Challenges of Human Resources Allocation in Industry 4.0

Information ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 120
Author(s):  
Majid Ziaei Nafchi ◽  
Hana Mohelská
Keyword(s):  
Czech Republic ◽  
Human Resources ◽  
Industry 4.0 ◽  
Industrial Revolution ◽  
High Tech ◽  
Economic Sectors ◽  
The Czech Republic ◽  
Services Sector ◽  
Productivity And Competitiveness ◽  
Near Future

The emergence of the fourth industrial revolution (Industry 4.0, hereinafter I 4.0) has led to an entirely fresh approach to production, helping to enhance the key industrial processes and therefore increase the growth of labor productivity and competitiveness. Simultaneously, I 4.0 compels changes in the organization of work and influences the lives of employees. The paper intends to construct a model for predicting the allocation of human resources in the sectors of the national economy of the Czech Republic in connection with I 4.0. The model used in this research visualizes the shift of labor in the economic sectors of the Czech Republic from the year 2013 to the following years in the near future. The main contribution of this article is to show the growth of employment in the high-tech services sector, which will have an ascending trend.

Prevalence and genetic diversity of viruses associated with rugose wood complex in Greek vineyards

Plant Disease ◽  
2021 ◽  
Author(s):  
Chrysoula Orfanidou ◽  
Kalliopi Moraki ◽  
Polina Panailidou ◽  
Leonidas Lotos ◽  
Asimina T Katsiani ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
First Record ◽  
Grapevine Virus ◽  
Multiple Introductions ◽  
Worldwide Distribution ◽  
Grapevine Virus A ◽  
Transmission Modes ◽  
Virus Incidence ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting

Rugose wood is one of the most important disease syndromes of grapevine and it has been associated with at least three viruses: grapevine rupestris stem pitting associated virus (GRSPaV), grapevine virus A (GVA) and grapevine virus B (GVB). All three viruses show a worldwide distribution pattern, and their genetic composition has been the focus of extensive research over the past years. Despite their first record in Greece almost 20 years ago, there is a lack of knowledge on the distribution and genetic variability of their populations in Greek vineyards. In this context, we investigated the distribution of GRSPaV, GVA and GVB in rootstocks, self-rooted and grafted grapevine cultivars, originating from different geographic regions that are representing important viticultural areas of Greece. Three new RT-PCR assays were developed for the reliable detection of GRSPaV, GVA and GVB. Our results indicated that GVA is the most prevalent in Greek vineyards, followed by GRSPaV and GVB. However, virus incidence differed among self-rooted and grafted grapevine cultivars or rootstocks tested. Selected isolates from each virus were further molecularly characterized to determine their phylogenetic relationships. All three viruses exhibited high nucleotide diversity, which was depicted in the constructed phylogenetic trees. Isolates from Greece were placed in various phylogroups, reinforcing the scenario of multiple introductions of GVA, GVB and GRSPaV in Greece and highlighting the effect of different transmission modes in the evolutionary course of the three viruses.

scholarly journals Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B

2008 ◽  
Vol 43 (10) ◽  
pp. 1405-1411 ◽  
Author(s):  
Paula Radaelli ◽  
Thor Vinícius Martins Fajardo ◽  
Osmar Nickel ◽  
Marcelo Eiras ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Virus Disease ◽  
Disease Diagnosis ◽  
Coat Proteins ◽  
Grapevine Virus ◽  
Western Blots ◽  
E Coli ◽  
Sds Page ◽  
Grapevine Virus B ◽  
Polyclonal Antisera ◽  
Infected Grapevine

The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the corresponding recombinant proteins in Western blots and to detect GLRaV-2 and GVB in infected grapevine tissues, by indirect ELISA, discriminating healthy and infected grapevines with absorbances (A405) of 0.08/1.15 and 0.12/1.30, respectively. Expressing CP genes can yield high amount of viral protein with high antigenicity, and GLRaV-2 and GVB antisera obtained in this study can allow reliable virus disease diagnosis.

scholarly journals Detecção do Grapevine virus A e Grapevine virus B por hibridização "dot-blot" com sondas moleculares não radioativas

2005 ◽  
Vol 30 (5) ◽  
pp. 538-542
Author(s):  
Andreia E Moreira ◽  
José O Gaspar ◽  
Hugo Kuniyuki
Keyword(s):  
Grapevine Virus ◽  
Rt Pcr ◽  
Dot Blot ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Vitis Spp ◽  
Kober Stem Grooving

O vírus A da videira (Grapevine virus A, GVA) e o vírus B da videira (Grapevirus virus B, GVB) estão associados à acanaladura do lenho de Kober ("Kober stem grooving") e ao fendilhamento cortical da videira ("grapevine corky bark"), respectivamente. Este trabalho descreve o uso de sondas moleculares de cDNA na detecção de isolados do GVA (GVA-SP) e do GVB (GVB-C-SP e GVB-I-SP) em videiras (Vitis spp.) e fumo (Nicotiana occidentalis). As sondas marcadas com digoxigenina foram produzidas por RT-PCR utilizando oligonucleotídeos específicos para os genes da proteína capsidial. Os RNA totais foram extraídos de 45 plantas de diversas variedades de videira e de 13 plantas de fumo inoculadas mecanicamente com o GVB. Os RNA extraídos das plantas infetadas, indexadas biologicamente, hibridizaram com as sondas, não se verificando reação com plantas sadias. Para confirmar os resultados de hibridização, foram também feitos testes de RT-PCR. A utilização de hibridização "dot-blot" com sondas de cDNA mostrou-se eficaz na detecção dos vírus com especificidade e sensibilidade, ressaltando-se que, preferencialmente, folhas maduras e ramos dormentes devem ser utilizados nos testes diagnósticos para o GVB e GVA, respectivamente.

scholarly journals Sanitary Status of the Grapevine Germplasm Collection in Republic of Srpska

2017 ◽  
Vol 17 (2) ◽  
pp. 143
Author(s):  
Duška Delić ◽  
Biljana Lolić ◽  
Gordana Đurić ◽  
Tatjana Jovanović-Cvetković
Keyword(s):  
Nested Pcr ◽  
Germplasm Collection ◽  
Elisa Test ◽  
Grapevine Fanleaf Virus ◽  
Pcr Rflp ◽  
Near Future ◽  
Infected Grapevine ◽  
Rflp Assay ◽  
Das Elisa

In July 2015, 179 grapevine plants belonging to 16 grapevine autochthonous cultivars were assessed for sanitary status using DAS ELISA test for the presence of: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2)and Grapevine leafroll-associated virus 3 (GLRaV-3). Furthermore, surveyfor the phytoplasma presence and laboratory analyses using nested-PCR/RFLP assay was conducted at the beginning of September 2015 on grapevine cultivars which were not positive in DAS ELISA test for the presence of the four viruses. Out of 179 tested plants with DAS ELISA test, 146 (81%) were positive for the presence of at least one virus. The most widespread viruses were GFLaV- 1 and GFLaV- 3 with approximately 80 % of grapevines infected. Nested–PCR/RFLP assay showed that out of 33 tested samples 2 were positive for the presence of phytoplasmas from 16SrXII group. Sanitation of infected grapevine cultivars is needed in near future.

scholarly journals The performance of the Czech labour market after the 2004 EU enlargement

2013 ◽  
Vol 58 (197) ◽  
pp. 79-94
Author(s):  
Wadim Strielkowski ◽  
Jan Hněvkovský
Keyword(s):  
Czech Republic ◽  
Labour Market ◽  
Eu Enlargement ◽  
Foreign Workers ◽  
Eu Accession ◽  
The Czech Republic ◽  
Eu Membership ◽  
Significant Change ◽  
Near Future ◽  
The Eu

This paper examines whether there has been a significant change in the performance of the Czech labour market after the Czech Republic?s EU Accession in May 2004. We analyse methodological changes of measuring unemployment caused by inevitable legislative adjustments and follow the development of the Czech labour market and the inflows of foreign workers to the Czech Republic over the past two decades. Our results show that the EU Accession resulted in simplifying foreigners? access to the Czech labour market and did not cause a significant change in its performance. Our findings might be of some relevance for the countries seeking EU Membership in the near future (e.g., Serbia or Montenegro).

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