The use of cardiac progenitor cells for transplantation in congenital heart disease and an innovative strategy for activating mitochondrial function in such cells

Abstract Background Pulmonary arterial hypertension (PAH) caused by congenital heart disease (CHD) is very common in clinics. Some studies have shown that PAH is related to the number of endothelial progenitor cells (EPCs), but there is no report on the relationship between PAH and the number of EPCs in children with CHD. Methods In this study, a total of 173 cases with CHD (from 0 to 6 years old) were collected. According to the mean pulmonary arterial pressure (mPAP) measured by right heart catheterization, these cases were divided into PAH groups (including high PAH group, mPAP> 25 mmHg, n = 32, and the middle PAH group, 20 mmHg ≤ mPAP≤25 mmHg, n = 30) and non-PAH group (mPAP< 20 mmHg, n = 111). Peripheral blood was taken for flow cytometry, and the number of EPCs (CD133+/KDR+ cells) was counted. The number of EPCs /μL of peripheral blood was calculated using the following formula: EPCs /μL = WBC /L × lymphocytes % × EPCs % × 10− 6. Results The median EPCs of the non-PAH group, middle PAH group and high PAH group is 1.86/μL, 1.30 /μL and 0.98/μL, respectively. The mPAP decreases steadily as the level of EPCs increases (P < 0.05). After adjustment of gender, age and BMI, the number of EPCs was significantly associated with a decreased risk of high PAH (OR = 0.37, 95% CI: 0.16–0.87, P < 0.05). However, EPCs was not significantly associated with middle PAH (P > 0.05). Conclusion The findings revealed that the EPCs and high PAH in patients with CHD correlate significantly and EPCs may become an effective treatment for PAH in patients with CHD. EPCs may be a protective factor of high PAH for children with CHD.

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The Circulating Level of Endothelial Progenitor Cells After Transcatheter Closure of Congenital Heart Disease in Children

Pediatric Cardiology ◽

10.1007/s00246-013-0647-y ◽

2013 ◽

Vol 34 (6) ◽

pp. 1344-1349 ◽

Cited By ~ 4

Author(s):

Ming-Guo Xu ◽

Xiang-Chun Meng ◽

Bo-Ning Li ◽

Cong Liu

Keyword(s):

Congenital Heart Disease ◽

Heart Disease ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Congenital Heart ◽

Transcatheter Closure ◽

Endothelial Progenitor

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The relationship between endothelial progenitor cells and pulmonary arterial hypertension in children with congenital heart disease

10.21203/rs.2.16843/v3 ◽

2019 ◽

Author(s):

Hong-Xiao Sun ◽

Guo-Ju Li ◽

Zhan-Hui Du ◽

Zhen Bing ◽

Zhi-Xian Ji ◽

...

Keyword(s):

Congenital Heart Disease ◽

Heart Disease ◽

Pulmonary Arterial Hypertension ◽

Arterial Hypertension ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Peripheral Blood ◽

Congenital Heart ◽

Pulmonary Arterial ◽

The Relationship

Abstract Background: Pulmonary arterial hypertension (PAH) caused by congenital heart disease (CHD) is very common in clinics. Some studies have shown that PAH is related to the number of endothelial progenitor cells (EPCs), but there is no report on the relationship between PAH and the number of EPCs in children with CHD. Methods: In this study, a total of 173 cases with CHD (from 0 to 6 years old) were collected. According to the mean pulmonary arterial pressure (mPAP) measured by right heart catheterization, these cases were divided into PAH groups (including high PAH group, mPAP>25mmHg, n=32, and the middle PAH group, 20mmHg≤mPAP≤25mmHg, n=30) and non-PAH group (mPAP<20mmHg, n=111). Peripheral blood was taken for flow cytometry, and the number of EPCs (CD133+/KDR+ cells) was counted. The number of EPCs /μL of peripheral blood was calculated using the following formula: EPCs /μL =WBC /L × lymphocytes % × EPCs % × 10-6. Results: The median EPCs of the non-PAH group, middle PAH group and high PAH group is 1.86/μL, 1.30 /μL and 0.98/μL, respectively. The mPAP decreases steadily as the level of EPCs increases (P<0.05). After adjustment of gender, age and BMI, the number of EPCs was significantly associated with a decreased risk of high PAH (OR=0.37, 95% CI: 0.16-0.87, P<0.05). However, EPCs was not significantly associated with middle PAH (P>0.05). Conclusion: The findings revealed that the EPCs and high PAH in patients with CHD correlate significantly and EPCs may become an effective treatment for PAH in patients with CHD. EPCs may be a protective factor of high PAH for children with CHD.

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The relationship between endothelial progenitor cells and pulmonary arterial hypertension in children with congenital heart disease

10.21203/rs.2.16843/v2 ◽

2019 ◽

Author(s):

Hong-Xiao Sun ◽

Guo-Ju Li ◽

Zhan-Hui Du ◽

Zhen Bing ◽

Zhi-Xian Ji ◽

...

Keyword(s):

Congenital Heart Disease ◽

Heart Disease ◽

Pulmonary Arterial Hypertension ◽

Arterial Hypertension ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Peripheral Blood ◽

Congenital Heart ◽

Pulmonary Arterial ◽

The Relationship

Abstract Background: Pulmonary arterial hypertension (PAH) caused by congenital heart disease (CHD) is very common in clinical. Some studies have shown that PAH is related to the number of endothelial progenitor cells (EPCs), but there is no report on the relationship between PAH and the number of EPCs in children with CHD. Methods: In this study, a total of 173 cases with CHD (from 0 to 6 years old) were collected. According to the mean pulmonary arterial pressure (mPAP) measured by right heart catheterization, these cases were divided into PAH groups (including high PAH group, mPAP>25mmHg, n=32, and the middle PAH group, 20mmHg≤mPAP≤25mmHg, n=30) and non-PAH group (mPAP<20mmHg, n=111). Peripheral blood was taken for flow cytometry, and the number of EPCs(CD133+/KDR+ cells) was counted. The number of EPCs /μL of peripheral blood was calculated using the following formula: EPCs /μL =WBC /L × lymphocytes % × EPCs % × 10-6. Results: The median EPCs of the non-PAH group, middle PAH group and high PAH group is 1.86/μL, 1.30 /μL and 0.98/μL, respectively. The mPAP decreases steadily as the level of EPCs increases (P<0.05). After adjustment of gender, age and BMI, the number of EPCs was significantly associated with a decreased risk of high PAH (OR=0.37, 95% CI: 0.16-0.87, P<0.05). However, EPCs was not significantly associated with middle PAH (P>0.05). Conclusion: The findings revealed that the EPCs and high PAH in patients with CHD correlate significantly and EPCs may become an effective treatment for PAH in patients with CHD. EPCs may be a protective factor of high PAH for children with CHD

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MOBILIZATION OF OCT-4+ VERY SMALL EMBRYONIC LIKE STEM CELLS, HEMATOPOIETIC STEM CELLS AND ENDOTHELIAL PROGENITOR CELLS IN CHILDREN WITH CONGENITAL HEART DISEASE UNDERGOING CARDIAC SURGERY

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(12)60969-3 ◽

2012 ◽

Vol 59 (13) ◽

pp. E968

Author(s):

Wojciech Wojakowski ◽

Kolcz Jacek ◽

Zuba-Surma Ewa ◽

Drukala Justyna ◽

Mariusz Ratajczak ◽

...

Keyword(s):

Stem Cells ◽

Congenital Heart Disease ◽

Cardiac Surgery ◽

Heart Disease ◽

Hematopoietic Stem Cells ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Congenital Heart ◽

Endothelial Progenitor ◽

Hematopoietic Stem

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The relationship between endothelial progenitor cells and pulmonary hypertension in children with congenital heart disease

10.21203/rs.2.16843/v1 ◽

2019 ◽

Author(s):

Hong-Xiao Sun ◽

Guo-Ju Li ◽

Zhan-Hui Du ◽

Zhen Bing ◽

Zhi-Xian Ji ◽

...

Keyword(s):

Congenital Heart Disease ◽

Pulmonary Hypertension ◽

Heart Disease ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Peripheral Blood ◽

Congenital Heart ◽

Endothelial Progenitor ◽

High Ph ◽

The Relationship

Abstract Background: To assess the relationship between endothelial progenitor cells (EPCs) in peripheral blood and pulmonary hypertension (PH) in children with congenital heart disease (CHD) and explore the possibility of applying EPCs to treatment of PH in children with CHD. Methods: In this study, a total of 173 cases with CHD (from 0 to 6 years old) were collected. According to the right heart catheterization of mean pulmonary arterial pressure (mPAP) results, these cases were divided into PH groups (including high PH group, n=32, and the middle PH group, n=30) and non-PH group (n=111). We took their peripheral blood for flow cytometry, and counted the number of EPCs(CD133+/KDR+ cells). The number of EPCs /μL of peripheral blood was calculated using the following formula: EPCs /μL =WBC /L × lymphocytes % × EPCs % × 10-6. Results: The median EPCs of the non-PH group, middle PH group and high PH group are 1.86/μL, 1.30 /μL and 0.98/μL, respectively. The mPAP decreases steadily as the level of EPCs increases (P<0.05). After adjustment of gender, age and BMI, the EPCs was significantly associated with a decreased risk of high PH (OR=0.37, 95%CI: 0.16-0.87, P<0.05). However, EPCs was not significantly associated with middle PH (P>0.05). Conclusion: The findings revealed that the EPCs and high PH in CHD patients correlate significantly and EPCs may become an effective treatment for PH in CHD patients.EPCs may be a protective factor of high PH for children with CHD.

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Detection of TUNEL-positive cardiomyocytes and C-kit-positive progenitor cells in children with congenital heart disease

Journal of Molecular and Cellular Cardiology ◽

10.1016/j.yjmcc.2007.05.011 ◽

2007 ◽

Vol 43 (3) ◽

pp. 254-261 ◽

Cited By ~ 16

Author(s):

Hisashi Sato ◽

Isao Shiraishi ◽

Tetsuro Takamatsu ◽

Kenji Hamaoka

Keyword(s):

Congenital Heart Disease ◽

Heart Disease ◽

Progenitor Cells ◽

Congenital Heart

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Widespread endotheliopathy in adults with cyanotic congenital heart disease

Cardiology in the Young ◽

10.1017/s1047951114000262 ◽

2014 ◽

Vol 25 (3) ◽

pp. 511-519 ◽

Cited By ~ 14

Author(s):

Rachael L. Cordina ◽

Shirley Nakhla ◽

Shamus O’Meagher ◽

John Leaney ◽

Stuart Graham ◽

...

Keyword(s):

Nitric Oxide ◽

Congenital Heart Disease ◽

Heart Disease ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Congenital Heart ◽

Asymmetric Dimethylarginine ◽

Cyanotic Congenital Heart Disease ◽

Endothelial Progenitor ◽

Flow Mediated Dilatation

AbstractIntroduction: Cyanotic congenital heart disease is associated with functional limitation and vascular events. The nature and extent of endothelial dysfunction in cyanotic adults is poorly understood. We sought to characterise endothelial function in this setting. Methods: A total of fourteen adults with cyanotic congenital heart disease (40±3 years) together with age- and sex-matched healthy controls underwent assessment of nitric oxide-dependent vascular responses, including flow-mediated dilatation of the brachial artery and dynamic vessel analysis of the retina in response to flickering light. Plasma levels of the endothelium-derived vasoconstrictor endothelin-1 and the nitric oxide antagonist, asymmetric dimethylarginine, were measured. Circulating endothelial progenitor cells were assessed by flow cytometry. Results: Flow-mediated dilatation was significantly lower in cyanosed adults than controls (4.0±0.8 versus 7.2±1.0%, p=0.019, n=11 per group). Retinal arterial and venous dilatory responses were also impaired (2.9±0.8 versus 5.0±0.6%, p=0.05 and 3.4±0.3 versus 5.2±0.7%, p=0.04, n=13). Serum levels of endothelin-1 and asymmetric dimethylarginine were higher in cyanosed adults (3.0±0.6 versus 1.1±0.1 pg/ml, p=0.004 and 0.68±0.05 versus 0.52±0.02 μmol/L, p=0.03, n=11). Endothelial progenitor cells (CD34+CD45dimCD133+KDR+) were reduced in those with chronic cyanosis (17±4 versus 40±6 per million white blood cells, p=0.005, n=11). Conclusions: Endothelial function is impaired in the systemic arteries and retinal vessels in adults with cyanotic congenital heart disease, suggesting a widespread endotheliopathy. Diminished numbers of endothelial progenitor cells might potentially contribute to these observations.

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