Wnt ligands 3a and 5a regulate proliferation and migration in human fetal liver progenitor cells

In the group of patients (n = 21, mean age 54 ± 5.8 years) with chronic lower limb ischemia stage IIB who were non-liable for reconstructiverestoration surgery, we have established positive clinical effects of local transplantation of human fetal liver progenitor cells. Complex examination following 1, 3, 6 and 12 months after transplantation included duplex scanning of limb arteries, x-ray contrast arteriography and laser Doppler flowmetry as well as measuring pain-free walking and evaluating life quality based on individual questionnaire data.Owing to the transplant “Cryopreserved human fetal liver progenitor cells” the patients demonstrated stable increase of life quality index and pain-free walking as well as improvement of general health allowing assign them to the group of patients with lower ischemia stage, quicker social rehabilitation and lesser risk of disabling surgery (р < 0.05). Also, there were observations of improved microcirculation in the ischemic extremities owing to activation of endothelium-independent mechanisms of vasodilatation, reduced myotonus and neurotonus of the pre-capillaries and improved endothelium-dependent influence on the microhaemodynamic and, hence, an increased reserve capillary blood flow (p < 0.05).Analysis of the obtained results indicates prospects and effectiveness of using fetal liver cells transplantation in the patients who are not liable for surgical reconstruction of the vascular bed.

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33. Cryosensitivity of hematopoietic and mesenchymal stem/progenitor cells derived from human fetal liver

Cryobiology ◽

10.1016/j.cryobiol.2006.10.034 ◽

2006 ◽

Vol 53 (3) ◽

pp. 381-382

Author(s):

Yuri A. Petrenko ◽

Nataliya G. Skorobogatova ◽

Rhodri E. Jones ◽

Alexander Y. Petrenko

Keyword(s):

Progenitor Cells ◽

Fetal Liver ◽

Human Fetal Liver

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Protective Effect of Silymarin against Rapamycin-induced Apoptosis and Proliferation Inhibition in Endothelial Progenitor Cells

Natural Product Communications ◽

10.1177/1934578x1501000211 ◽

2015 ◽

Vol 10 (2) ◽

pp. 1934578X1501000 ◽

Cited By ~ 2

Author(s):

Peng Zhang ◽

Guohua Han ◽

Pei Gao ◽

Kun Qiao ◽

Yusheng Ren ◽

...

Keyword(s):

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Mononuclear Cells ◽

Control Group ◽

Dependent Manner ◽

Endothelial Progenitor ◽

Concentration Dependent Manner ◽

Inhibition Of Proliferation ◽

And Migration ◽

Proliferation And Migration

For this study, peripheral blood samples were collected from human volunteers. Mononuclear cells (MNC) were separated by density centrifugation and were induced to differentiate into endothelial progenitor cells (EPCs) in vitro. Different concentrations of rapamycin and silymarin were introduced to the EPCs over 24 hours and then EPCs were analyzed for proliferation, migration, apoptosis and angiogenesis. Compared with the control group, rapamycin (1, 10, 100 ng/mL) inhibited the proliferation and migration of EPCs in a concentration dependent manner ( P<0.05). Silymarin (50, 100 μg/mL) enhanced the proliferation and migration of EPCs and inhibited apoptosis in a concentration dependent manner ( P<0.05). By adding rapamycin (1 ng/mL) and silymarin (25, 50, 100 μg/mL) over 24 hours, silymarin inhibited the pro-apoptotic effect of rapamycin on EPCs, and reversed the inhibition of proliferation, migration and angiogenesis of EPCs by rapamycin ( P<0.05).

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