Molecular Genetic Analysis of the Complete Genome of Tick-Borne Encephalitis Virus (Siberia Subtype): Modern Kolarovo-2008 Isolate

Determined is the complete genome sequence of Kolarovo-2008 strain (Siberia subtype) of Tick-borne encephalitis virus (TBEV), isolated from a tick in the suburbs of the Tomsk city. Nucleotide sequence analysis testifies of the fact that the level of genetic differences within the Siberian subtype of TBEV amounts to 10 % of the nucleotide sequence and to 7 % of amino-acid sequence for certain virus genes. 3'-HTO of the genome of Siberian subtype has the highest rate of variability and the homology level ranging from 65 to 97 %. Kolarovo-2008 and Vasilchenko (isolated in Novosibirsk in 1969) strains have the highest level of genome homology. The level of dissimilarity between the two Tomsk strains is substantially higher: the total number of amino-acid substitutions in Tomsk Zausaev and Kolarovo-2008 strains equals to 124, and 3'HTO level of homology is 79 %. Identified genetic variability of the Siberian subtype of TBEV is of a great importance for further development and enhancement of tick-borne encephalitis virus diagnostics.

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Nucleotide sequence of the genome and complete amino acid sequence of the polyprotein of tick-borne encephalitis virus

Virology ◽

10.1016/0042-6822(90)90073-z ◽

1990 ◽

Vol 174 (1) ◽

pp. 250-263 ◽

Cited By ~ 87

Author(s):

Alexander G. Pletnev ◽

Vladimir F. Yamshchikov ◽

Vladimir M. Blinov

Keyword(s):

Amino Acid ◽

Nucleotide Sequence ◽

Amino Acid Sequence ◽

Encephalitis Virus ◽

Complete Amino Acid Sequence ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus

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Complete Genome Sequence of Tick-Borne Encephalitis Virus Strain A104 Isolated from a Yellow-Necked Mouse (Apodemus flavicollis) in Austria

Genome Announcements ◽

10.1128/genomea.00564-13 ◽

2013 ◽

Vol 1 (4) ◽

Cited By ~ 4

Author(s):

S. Frey ◽

S. Essbauer ◽

G. Zoller ◽

B. Klempa ◽

M. Weidmann ◽

...

Keyword(s):

Genome Sequence ◽

Virus Strain ◽

Complete Genome Sequence ◽

Complete Genome ◽

Encephalitis Virus ◽

Apodemus Flavicollis ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus ◽

Yellow Necked Mouse

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Reconsidering the classification of tick-borne encephalitis virus within the Siberian subtype gives new insights into its evolutionary history

Infection Genetics and Evolution ◽

10.1016/j.meegid.2017.09.014 ◽

2017 ◽

Vol 55 ◽

pp. 159-165 ◽

Cited By ~ 38

Author(s):

S.Y. Kovalev ◽

T.A. Mukhacheva

Keyword(s):

Evolutionary History ◽

Encephalitis Virus ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus ◽

Siberian Subtype

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Characterization and complete genome sequences of high- and low-virulence variants of tick-borne encephalitis virus

Journal of General Virology ◽

10.1099/0022-1317-77-5-1035 ◽

1996 ◽

Vol 77 (5) ◽

pp. 1035-1042 ◽

Cited By ~ 58

Author(s):

G. Wallner ◽

C. W. Mandl ◽

M. Ecker ◽

H. Holzmann ◽

K. Stiasny ◽

...

Keyword(s):

Complete Genome ◽

Encephalitis Virus ◽

Genome Sequences ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus

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Spontaneous Mutations Restore the Viability of Tick-Borne Encephalitis Virus Mutants with Large Deletions in Protein C

Journal of Virology ◽

10.1128/jvi.77.1.443-451.2003 ◽

2003 ◽

Vol 77 (1) ◽

pp. 443-451 ◽

Cited By ~ 46

Author(s):

Regina M. Kofler ◽

Agnes Leitner ◽

Gabriel O'Riordain ◽

Franz X. Heinz ◽

Christian W. Mandl

Keyword(s):

Amino Acid ◽

Protein C ◽

Deletion Mutant ◽

Hydrophobic Interactions ◽

Encephalitis Virus ◽

Sequence Element ◽

Hydrophobic Domain ◽

Large Deletions ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus

ABSTRACT The capsid protein, C, of tick-borne encephalitis virus has recently been found to tolerate deletions up to a length of 16 amino acid residues that partially removed the central hydrophobic domain, a sequence element conserved among flaviviruses which may be crucial for virion assembly. In this study, mutants with deletion lengths of 19, 21, 27, or 30 residues, removing more or all of this hydrophobic domain, were found to yield viable virus progeny, but this was without exception accompanied by the emergence of additional mutations within protein C. These point mutations or sequence duplications were located downstream of the engineered deletion and generally increased the hydrophobicity, suggesting that they may compensate for the loss of the central hydrophobic domain. Two of the second-site mutations, together with the corresponding deletion, were introduced into a wild-type genetic backbone, and the analysis of these “double mutants” provided direct evidence that the viability of the deletion mutant indeed depended on the presence of the second-site mutation. Our results corroborate the notion that hydrophobic interactions of protein C are essential for the assembly of infectious flavivirus particles but rule out the possibility that individual residues of the central hydrophobic domain are absolutely required for infectivity. Furthermore, the double mutants were found to be highly attenuated and capable of inducing a protective immune response in mice at even lower inoculation doses than the previously characterized 16-amino-acid-residue deletion mutant, suggesting that the combination of large deletions and second-site mutations may be a superior way to generate safe, attenuated flavivirus vaccine strains.

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