scholarly journals Genetic Diversity Analysis of Indonesian Pigmented Rice Accessions Based on Functional Molecular Markers

2021 ◽  
Vol 17 (1) ◽  
pp. 25
Author(s):  
Andari Risliawati ◽  
Yusi N. Andarini ◽  
Rerenstradika T. Terryana ◽  
Kristianto Nugroho ◽  
Puji Lestari
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Gene Bank ◽  
Diversity Analysis ◽  
Anthocyanin Content ◽  
Local Varieties ◽  
Pigmented Rice ◽  
Health Studies ◽  
Pericarp Color ◽  
Functional Molecular Markers

Pigmented rice is functional staple food that becomes popular because of its anthocyanin content which is beneficial for health. Studies on the diversity of the local variety of Indonesian pigmented rice accessions have been carried out, but are still limited to one region of germplasm origin. This study aimed to analyze the genetic diversity of local varieties of pigmented rice collections of the IAARD-ICABIOGRAD Gene Bank. A total of 93 pigmented rice accessions from 16 provinces in Indonesia were analyzed using 15 functional molecular markers of SSR, STS, and indel. The total alleles detected were 115 with an average per locus of genetic diversity value of 0.71. There were five markers with PIC values >0.75, i.e. RM167, RM223, R8M33, R10M10, and GBSS1. The accessions were divided into two main groups based on their pericarp color. It is necessary to analyze the physicochemical content of the local rice accessions to complement the existing diversity information and identify potential pigmented rice accessions with high palatability.

Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis

2012 ◽  
Vol 92 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
S. C. Debnath ◽  
Y. L. Siow ◽  
J. Petkau ◽  
D. An ◽  
N. V. Bykova
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Activity ◽  
Molecular Markers ◽  
Molecular Techniques ◽  
Diversity Analysis ◽  
Full Potential ◽  
Genetic Diversity Analysis ◽  
Wide Range ◽  
Berry Crops ◽  
Improvement Programs

Debnath, S. C., Siow, Y. L., Petkau, J., An, D. and Bykova, N. V. 2012. Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant Sci. 92: 1121–1133. An improved understanding of important roles of dietary fruits in maintaining human health has led to a dramatic increase of global berry crop production. Berry fruits contain relatively high levels of vitamin C, cellulose and pectin, and produce anthocyanins, which have important therapeutic values, including antitumor, antiulcer, antioxidant and anti-inflammatory activities. There is a need to develop reliable methods to identify berry germplasm and assess genetic diversity/relatedness for dietary properties in berry genotypes for practical breeding purposes through genotype selection in a breeding program for cultivar development, and proprietary-rights protection. The introduction of molecular biology techniques, such as DNA-based markers, allows direct comparison of different genetic materials independent of environmental influences. Significant progress has been made in diversity analysis of wild cranberry, lowbush blueberry, lingonberry and cloudberry germplasm, and in strawberry and raspberry cultivars and advanced breeding lines developed in Canada. Inter simple sequence repeat (ISSR) markers detected an adequate degree of polymorphism to differentiate among berry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current berry improvement programs. Although multiple factors affect antioxidant activity, a wide range of genetic diversity has been reported in wild and cultivated berry crops. Diversity analysis based on molecular markers did not agree with those from antioxidant activity. The paper also discusses the issues that still need to be addressed to utilize the full potential of molecular techniques including expressed sequence tag-polymerase chain reaction (EST-PCR) analysis to develop improved environment-friendly berry cultivars suited to the changing needs of growers and consumers.

Congruence between morphological and molecular markers for genetic diversity analysis applied to forage palm genotypes propagated via bioreactors

2020 ◽  
Vol 147 ◽  
pp. 112230
Author(s):  
Selma Silva Rocha ◽  
Luciana Cardoso Nogueira Londe ◽  
Samy Pimenta ◽  
Maurício Mendes Cardoso ◽  
Nívio Poubel Gonçalves ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

scholarly journals Applications of molecular markers in Genetic Diversity Studies of maize

2020 ◽  
Vol 37 (1) ◽  
pp. 101-108
Author(s):  
Degife Asefa Zebire
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Diversity Analysis ◽  
Crop Species ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
Diversity Studies ◽  
Genetic Diversity Study

Molecular markers are efficient for exploiting variations in genotypes as they are not influenced by environmental factors and also speed up breeding programs. They are used to detect large numbers of distinct divergence between genotypes at the DNA level. Genetic diversity study helps to estimate the relationship between inbred lines to make the best hybrid combinations. Lines which are clustered in different heterotic groups are considered as the best hybrid combinations to carry out further breeding activities. Molecular markers are used to meet a number of objectives, including genetic diversity analysis and prediction of hybrid performances in divergent crop species. Agro-morphological and molecular markers have been utilized to study genetic diversity so far. In maize, the uses of molecular markers are important for the evaluation of genetic diversity of inbred lines and in clustering them into heterotic groups. These markers determine genetic similarity of the lines and are used to assess the genetic diversity of maize. Molecular markers have proven valuable for genetic diversity analysis of many crop species and genetically diverse lines are important to improve hybrid breeding. Keyword: Molecular marker; Genetic diversity; Genetic variation, Diversity Array technology; cluster analysis

Retrotransposon-based molecular markers for linkage and genetic diversity analysis in wheat

2003 ◽  
Vol 271 (1) ◽  
pp. 91-97 ◽  
Author(s):  
R. A. Queen ◽  
B. M. Gribbon ◽  
C. James ◽  
P. Jack ◽  
A. J. Flavell
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

scholarly journals Genetic diversity analysis of Rose varieties from Grandiflora group based on ISSR molecular markers

2021 ◽  
Vol 38 ◽  
pp. 00140
Author(s):  
Sophia S. Yudanova ◽  
Svetlana A. Plugatar ◽  
Zinaida K. Klimenko ◽  
Vera K. Zykova ◽  
Olena L. Rubtsova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Morphometric Characters ◽  
Climate Conditions ◽  
Genetic Diversity Analysis ◽  
Statistical Comparison ◽  
Separate Branch ◽  
Issr Molecular Markers ◽  
Continental Climate

Kinship and genetic diversity determination among six rose varieties from the Grandiflora group were carried out using ISSR thechnics. The studied varities were divided into 3 clades: I) ‘Lezginka’, ‘Queen Elizabeth’ and ‘Koralovy surpriz’ varieties; II) ‘Gurzuf’ and ‘Love’ varieties; III) ‘Komsomolsky ogonek’ variety formed a separate branch. This division into clades was confirmed by a statistical comparison of morphometric characters. On the basis of the obtained data, it can be concluded that the analysis using the selected primer group is well-suited for differentiation rose varieties into groups, which makes it possible, for one thing, to determine the genetic distance between varieties, and for another, to use these data in the future by certification of the varieties promising by resistance characters to the continental climate conditions.

Genetic Diversity Analysis in Grevillea robusta using ISSR Molecular Markers

2019 ◽  
Vol 145 (3) ◽  
pp. 260
Author(s):  
Priyanka Parmar ◽  
Aman Dabral ◽  
R. K. Meena ◽  
Shailesh Pandey ◽  
Rama Kant ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Grevillea Robusta ◽  
Genetic Diversity Analysis ◽  
Issr Molecular Markers
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