scholarly journals Isolation and characterization of Infectious bursal disease virus VP2 gene isolated from Tirupati region of Andhra Pradesh, India.

2017 ◽  
Vol 5 (4) ◽  
pp. 79
Author(s):  
Harshavardhan Reddy Parapatla ◽  
Kiran Kumar J ◽  
Sunil Kumar Myla ◽  
Charitha Devi Mekala
Keyword(s):  
Andhra Pradesh ◽  
Variable Region ◽  
Viral Disease ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Vp2 Gene ◽  
Isolation And Characterization ◽  
Bursal Disease ◽  
Hyper Variable Region

<p>Infectious bursal disease (IBD) is a highly contagious viral disease of birds characterized by lesions in the bursa of Fabricius, immunosuppression, severe morbidity and mortality. Due to amino acids substitutions in this region results in change in the topography of hyper variable region of VP2, where most of the neutralizing epitopes are present. Cloning and characterization of hyper variable region of prevalent strains of IBDV in this part of the country will provide basis for development of better vaccine for future. In the present study, we cloned four full length sequences of IBDV-VP2 with hyper variable region and found that all the cloned sequences have similar epitopes. Phylogenetic analysis showed that they are closely related to each other and are also closely related to strains of Luxembourg, China, and USA. This study will enhance the knowledge on IBDV circulating strains in this part of the country.</p>

scholarly journals Studi Lapang: Penegakan Diagnosis Infectious Bursal Disease (IBD) Pada Ayam Broiler

2019 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Aan Awaludin ◽  
Yudhi Ratna Nugraheni ◽  
Theo Mahiseta Syahniar ◽  
Dyah Laksito Rukmi ◽  
Agus Hadi Prayitno ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Care Management ◽  
Clinical Symptoms ◽  
Viral Disease ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Commercial Farms ◽  
The One ◽  
Organ Changes

Infectious Bursal Disease (IBD), also called Gumboro, was disease which attacked cells in the bursa of fabricius, causing interference with the chicken's immune system or immunosuppressive. IBD was the one of viral disease that often attacks chickens in the field. The aim of this study was to determine the diagnosis of IBD by through at clinical symptoms and necropsy that can still be relevant on in the field. The diagnosis of IBD correctly, cheaply, easily and quickly in the field is very important to optimize the health care management and evaluation program. The method used was by observing clinical symptoms of broiler chickens in commercial farms suspected of contracting IBD and observing post-death organs (necropsy). The object of necropsy was 5 samples of broiler chickens from the farm. Data was analyzed descriptively. The results of this study was the broiler chickens that infected with IBD could be diagnosed through clinical symptoms and post-death organ changes, so that the diagnosis for IBD cases in the field used the observation of clinical symptoms and necropsy are still relevant.

scholarly journals Studi Lapang: Penegakan Diagnosis Infectious Bursal Disease (IBD) Pada Ayam Broiler

2019 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Aan Awaludin ◽  
Yudhi Ratna Nugraheni ◽  
Theo Mahiseta Syahniar ◽  
Dyah Laksito Rukmi ◽  
Agus Hadi Prayitno ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Care Management ◽  
Clinical Symptoms ◽  
Viral Disease ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Commercial Farms ◽  
The One ◽  
Organ Changes

Infectious Bursal Disease (IBD), also called Gumboro, was disease which attacked cells in the bursa of fabricius, causing interference with the chicken's immune system or immunosuppressive. IBD was the one of viral disease that often attacks chickens in the field. The aim of this study was to determine the diagnosis of IBD by through at clinical symptoms and necropsy that can still be relevant on in the field. The diagnosis of IBD correctly, cheaply, easily and quickly in the field is very important to optimize the health care management and evaluation program. The method used was by observing clinical symptoms of broiler chickens in commercial farms suspected of contracting IBD and observing post-death organs (necropsy). The object of necropsy was 5 samples of broiler chickens from the farm. Data was analyzed descriptively. The results of this study was the broiler chickens that infected with IBD could be diagnosed through clinical symptoms and post-death organ changes, so that the diagnosis for IBD cases in the field used the observation of clinical symptoms and necropsy are still relevant.

scholarly journals Isolation and Characterization of Plaque Variants of Infectious Bursal Disease Virus

1988 ◽  
Vol 41 (12) ◽  
pp. 856-861
Author(s):  
KAZUE SAIJO ◽  
MINORU HIGASHIHARA ◽  
MASAMI HIRUMA ◽  
YOSHIO EIGUCHI ◽  
YUJIRO FUJISAKI
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Isolation And Characterization ◽  
Bursal Disease

scholarly journals Isolation and Characterization of Virulent Strain of Infectious Bursal Disease Virus from Broiler Birds in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 27-29
Author(s):  
MIA Begum ◽  
MA Islam ◽  
MER Bhuiyan ◽  
S Akter ◽  
M Rahman
Keyword(s):  
Chicken Embryo ◽  
Virulent Strain ◽  
Chicken Embryo Fibroblast ◽  
Infectious Bursal Disease ◽  
Agar Gel ◽  
Experimental Conditions ◽  
Isolation And Characterization ◽  
Bursal Disease ◽  
Polyclonal Serum

The bursa Fabricius of 50 dead broiler birds aged between 2 to 5 weeks were collected from three different private and Bangladesh Agricultural University (BAU) poultry farms for isolation of infectious bursal disease (IBD) virus during the period from August to September 2002. Each of the collected bursa was stored at -20°C until processed for the isolation of virus in chicken embryo fibroblast (CEF) cell culture. Of the 50 bursal samples cultured in the chicken embryo fibroblast (CEF) cells, of which 40 (80%) bursal samples were found positive for IBD virus. Each of the 40 isolated IBD virus was characterized as IBD virus M6 strain using type specific polyclonal serum raised in chickens by agar gel immunodiffusion test (AGIDT). These isolated IBD virus M6 strain caused 100% mortality in five weeks old layer chickens on experimental infection through intramuscular route of inoculation. The results of this study indicate that the local isolates of IBD virus are associated with high mortality in chickens under both the natural and experimental conditions in Bangladesh. Key words: Isolation, characterization; virulent strain; IBDV; broiler birds doi: 10.3329/bjvm.v2i1.1930 Bangl. J. Vet. Med. (2004). 2 (1) : 27-29

Sign in / Sign up

Export Citation Format

Share Document