scholarly journals Simultaneous Determination of Serotonin and 5-Hydroxyindole-3-acetic Acid in Human Urine by Automated Precolumn Derivatization and Semi-microbore Column Liquid Chromatography with Fluorescence Detection

2004 ◽  
Vol 20 (12) ◽  
pp. 1687-1690 ◽  
Author(s):  
Takashi YOSHITAKE ◽  
Ryuji IIZUKA ◽  
Kaoru FUJINO ◽  
Osamu INOUE ◽  
Kenji YAMAGATA ◽  
...  
Keyword(s):  
Column Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Human Urine ◽  
Precolumn Derivatization ◽  
Microbore Column

scholarly journals Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Grains by New Immunoaffinity Column/Liquid Chromatography

2004 ◽  
Vol 87 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Roswitha Göbel ◽  
Klaus Lusky
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Trifluoroacetic Acid ◽  
Detection Limits ◽  
Immunoaffinity Column ◽  
Recovery Rates

Abstract The simultaneous determination of mycotoxins was performed in 3 steps: extraction, cleanup, and detection. For extraction, a mixture of acetonitrile–water (60 + 40, v/v) was proved appropriate. For cleanup, a new Afla-Ochra-Zea immunoaffinity column was used. After derivatization with trifluoroacetic acid, the mycotoxins aflatoxins, ochratoxin A (OTA), and zearalenone (ZEA) were determined simultaneously by liquid chromatography with fluorescence detection. The detection limits in different matrixes after cleanup with the new immunoaffinity column were very low: aflatoxins, 0.002–0.7 μg/kg; OTA, 0.07–0.25 μg/kg; ZEA, 1–3 μg/kg. The limits of determination were: aflatoxins, 0.25 μg/kg; OTA, 0.5 μg/kg; ZEA, 5 μg/kg. The recovery rates for aflatoxins, OTA, and ZEA for rye and rice were between 86 and 93% when a 0.5 g sample matter per immunoaffinity column was used.

Determination of mevalonic acid in human urine by liquid chromatography with precolumn derivatization and fluorescence detection

1998 ◽  
Vol 365 (1-3) ◽  
pp. 205-212 ◽  
Author(s):  
Shuji Kawasaki ◽  
Tadayoshi Nagaoka ◽  
Yutaka Saisho ◽  
Atsushi Morimoto ◽  
Tsuneji Umeda
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Human Urine ◽  
Mevalonic Acid ◽  
Precolumn Derivatization
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