column switching
Recently Published Documents


TOTAL DOCUMENTS

1007
(FIVE YEARS 34)

H-INDEX

49
(FIVE YEARS 2)

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Meng Yuan ◽  
Xiaoyan Liu ◽  
Yue Sun ◽  
Linlin Wang ◽  
Pian Jin ◽  
...  

Separation power was limited when the conventional high-performance liquid chromatography (HPLC) fingerprinting method based on a single column was used to analyze very complex traditional Chinese medicine (TCM) preparations. In this research, a novel HPLC fingerprinting method based on column switching technology by using a single pump was established for evaluating the quality of Tianmeng oral liquid (TMOL). Twelve batches of TMOL samples were used for constructing HPLC fingerprints. Compared with the 16 common peaks in fingerprinting with a single column, 25 common peaks were achieved with two columns connected through a six-way valve. The similarity analysis combined with bootstrap method was applied to determine the similarity threshold, which was 0.992 to distinguish expired samples and unexpired samples. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) were also applied to classify the TMOL samples, and results revealed that expired and unexpired samples are classified into two categories. The HPLC fingerprinting based on column switching technology with better separation power and higher peak capacity could characterize chemical composition information more comprehensively, providing an effective and alternative method to control and evaluate the quality of TMOL, which would offer a valuable reference for other TCM preparations.


2021 ◽  
Vol 21 ◽  
pp. 100431
Author(s):  
Jonas Carneiro Cruz ◽  
Israel Donizeti de Souza ◽  
Caroline Fernandes Grecco ◽  
Eduardo Costa Figueiredo ◽  
Maria Eugênia Costa Queiroz

2021 ◽  
Vol 8 ◽  
Author(s):  
Ye Tian ◽  
Xiao-Meng Chong ◽  
Ying Liu ◽  
Ying Han ◽  
Chang-Qin Hu ◽  
...  

In this study, two isomeric impurities were identified in cefotiam hydrochloride injection preparation and were characterized. Column-switching HPLC-MS and NMR techniques were used to identify the impurity 1 as the Δ3(4) isomers of cefotiam. Using software-based calculations, it was predicted that neither of the isomeric impurities was embryotoxic. This study provides a reference for the production, storage, and quality control of cefotiam and related cephalosporin antibiotics.


Sign in / Sign up

Export Citation Format

Share Document