scholarly journals Spatial Resolution Improvement of Scanning Microscopy Based on Thermal Lens Spectroscopy with a Total-Internal-Reflection Arrangement

2005 ◽  
Vol 21 (4) ◽  
pp. 469-472 ◽  
Author(s):  
Takuya SHIMOSAKA ◽  
Masakazu IZAKO ◽  
Katsumi UCHIYAMA ◽  
Toshiyuki HOBO
Keyword(s):  
Spatial Resolution ◽  
Thermal Lens ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Scanning Microscopy ◽  
Thermal Lens Spectroscopy ◽  
Resolution Improvement ◽  
Reflection Arrangement

Development of a scanning microscopy by total internal reflection coupled with thermal lens spectroscopy

Micron ◽  
2004 ◽  
Vol 35 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Takuya Shimosaka ◽  
Kazutoshi Iwamoto ◽  
Masakazu Izako ◽  
Asa Suzuki ◽  
Katsumi Uchiyama ◽  
...  
Keyword(s):  
Thermal Lens ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Scanning Microscopy ◽  
Thermal Lens Spectroscopy

Plasmonic metal scattering immunoassay by total internal reflection scattering microscopy with nanoscale lateral resolution

2015 ◽  
Vol 51 (5) ◽  
pp. 945-947 ◽  
Author(s):  
Seungah Lee ◽  
Hyunung Yu ◽  
Seong Ho Kang
Keyword(s):  
Total Internal Reflection ◽  
Internal Reflection ◽  
Lateral Resolution ◽  
Scattering System ◽  
Resolution Improvement

The total internal reflection scattering system incorporating a z-nanopositioner is introduced to explore the precise immunoassay on gold-nanopattemed chips by lateral resolution improvement.

Attenuated Total Internal Reflection Infrared Mapping Microspectroscopy of Soft Materials

2000 ◽  
Vol 54 (2) ◽  
pp. 324-330 ◽  
Author(s):  
Lori L. Lewis ◽  
André J. Sommer
Keyword(s):  
Spatial Resolution ◽  
Total Internal Reflection ◽  
Soft Materials ◽  
Diffraction Limit ◽  
Internal Reflection ◽  
Attenuated Total Internal Reflection ◽  
Infrared Microspectroscopy ◽  
Soft Samples ◽  
Polymer Laminates

Attenuated total internal reflection (ATR) infrared mapping microspectroscopy of soft samples is reviewed and investigated by using cartridge-based germanium hemispheres. The study demonstrates the use of these devices for obtaining line scans or maps of soft pliable surfaces over an area of approximately 100 × 100 micrometers. An experimental determination of the spatial resolution by using a cross-sectioned polymer film showed a twofold improvement over transmission infrared microspectroscopy for sample sizes at the diffraction limit. Optical details of the devices are discussed in the context of ATR measurements in addition to their application for the study of polymer laminates often encountered in industry and forensics.

scholarly journals Three basic types of fluorescence microscopy and recent improvement

2021 ◽  
Vol 290 ◽  
pp. 01031
Author(s):  
Xinyi Wang ◽  
Yunyan Lai
Keyword(s):  
Confocal Microscopy ◽  
Molecular Biology ◽  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Future Perspectives ◽  
Recent Improvement ◽  
Basic Principles

Fluorescence microscopy is one of the most used imaging modalities in molecular biology and living specimens. To increase image contrast and spatial resolution, different type of fluorescence microscopy has been developed. This review introduces three main types of fluorescence microscopy: wild-field microscopy, confocal microscopy, and total internal reflection fluorescence microscopy. The basic principles are similar but with different modifications, which also indicates their attributes and limitation. The recent improvement on these microscopies is also discussed. Some most recent techniques show advance in overcoming common fluorescence microscopy's weakness, and future perspectives are also discussed.

Monitoring of Dye Adsorption Phenomena at a Silica Glass/Water Interface with Total Internal Reflection Coupled with a Thermal Lens Effect

2000 ◽  
Vol 72 (15) ◽  
pp. 3532-3538 ◽  
Author(s):  
Takuya Shimosaka ◽  
Toshiaki Sugii ◽  
Toshiyuki Hobo ◽  
J. B. Alexander Ross ◽  
Katsumi Uchiyama
Keyword(s):  
Thermal Lens ◽  
Silica Glass ◽  
Total Internal Reflection ◽  
Dye Adsorption ◽  
Internal Reflection ◽  
Water Interface ◽  
Lens Effect ◽  
Thermal Lens Effect ◽  
Adsorption Phenomena

Attenuated Total Internal Reflection Infrared Mapping Microspectroscopy Using an Imaging Microscope

2001 ◽  
Vol 55 (3) ◽  
pp. 252-256 ◽  
Author(s):  
André J. Sommer ◽  
Louis G. Tisinger ◽  
Curtis Marcott ◽  
Gloria M. Story
Keyword(s):  
Spatial Resolution ◽  
Focal Plane ◽  
Total Internal Reflection ◽  
Focal Plane Array ◽  
Internal Reflection ◽  
Array Detector ◽  
Combined System ◽  
Attenuated Total Internal Reflection ◽  
Rapid Acquisition ◽  
Infrared Microscope

Attenuated total internal reflection (ATR) infrared mapping microspectroscopy using an infrared microscope and a focal plane array is investigated and reported. The study demonstrates the advantages of conducting ATR microspectroscopy using a focal plane array detector. These benefits include the rapid acquisition of molecular specific images, ease of sample preparation, and increased spatial resolution. An experimental determination of the spatial resolution found that the combined system operates very close to the diffraction limit, and a 4 magnification factor associated with the germanium internal reflection element was realized. Experiments conducted on several polymer samples and a biological sample demonstrate the future viability of the method.

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