Isoelectric Focusing Sample Injection for Capillary Zone Electrophoresis in a Fused Silica Capillary

The protein separation was studied in capillary zone electrophoresis for preventing protein adsorption on the capillary wall. ß-lactoglobulin (pI: 4.83-5.4, Mr: 18.4 kDa), cytochrome c (pI: 9.59, Mr: 11.7 kDa) and ß-casein (pI: 4.6, Mr: 24 kDa) were used as protein models. Strong adsorption of the proteins occurred onto the capillary at a pH around their pIs. In order to prevent protein adsorption, PEG (Poly(ethylene glycol)) was investigated as an effective substance to stabilize the proteins native state and coat the bare fused-silica capillary surface. The presence of 32 mg/mL PEG in buffer solution in a pH range of 6.0 to 4.0 was successful to suppress protein adsorption during the separation. It can also be confirmed with the reproducibility of apparent EOF mobility with percentile RSD (Relative Standard Deviation) less than 2% in long-term measurement. Keywords: PEG, protein adsorption, CZE

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Separation of weak organic acids and bases by capillary zone electrophoresis in a sulfonated-polymer wall-modified open tubular fused-silica capillary

Journal of Chromatography A ◽

10.1016/s0021-9673(98)00125-3 ◽

1998 ◽

Vol 804 (1-2) ◽

pp. 337-348 ◽

Cited By ~ 11

Author(s):

Y Liu ◽

D.J Pietrzyk

Keyword(s):

Organic Acids ◽

Capillary Zone Electrophoresis ◽

Fused Silica ◽

Weak Organic Acids ◽

Zone Electrophoresis ◽

Fused Silica Capillary ◽

Capillary Zone ◽

Acids And Bases ◽

Polymer Wall ◽

Silica Capillary

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Determination of Chlorinated Phenols in Water and Soil by Capillary Zone Electrophoresis

Journal of AOAC International ◽

10.1093/jaoac/80.6.1308 ◽

1997 ◽

Vol 80 (6) ◽

pp. 1308-1314 ◽

Cited By ~ 1

Author(s):

Wayne E Rae ◽

Charles A Lucy

Keyword(s):

Capillary Zone Electrophoresis ◽

Fused Silica ◽

Soil Samples ◽

Chlorinated Phenols ◽

Relative Standard ◽

Zone Electrophoresis ◽

Water And Soil Samples ◽

Fused Silica Capillary ◽

Capillary Zone

Abstract A capillary zone electrophoresis (CZE) method was developed to separate and determine chlorinated phenols in water and soil samples. A mixture of 16 chlorinated phenols was resolved in 25 min by using a 77 cm (70 cm to detector) × 75 μm fused silica capillary with 0.015M tetraborate/0.045M phosphate (pH 7.3) buffer at 22 kV. Calibration linearities for water samples in the low parts-permillion range were good (correlation coefficient > 0.99) for all solutes except p-chlorophenol. Average precision was 17% relative standard deviation. Typical detection limits were in the 200 μg/L range. Recoveries of chlorinated phenols from synthetic soil samples with methanol were quantitative.

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Detection of Cymbidium Mosaic Potexvirus and Odontoglossum Ringspot Tobamovirus Using Immuno-Capillary Zone Electrophoresis

Phytopathology ◽

10.1094/phyto.1999.89.6.522 ◽

1999 ◽

Vol 89 (6) ◽

pp. 522-528 ◽

Cited By ~ 25

Author(s):

Alvin Jin-Cherng Eun ◽

Sek-Man Wong

Keyword(s):

Capillary Zone Electrophoresis ◽

Chenopodium Quinoa ◽

Diagnostic Technique ◽

Plant Viruses ◽

Fused Silica ◽

Zone Electrophoresis ◽

Fused Silica Capillary ◽

Capillary Zone ◽

Antibody Complex ◽

Multiple Samples

Immuno-capillary zone electrophoresis (I-CZE) is a technique that combines the specificity afforded by serological assays with the sensitivity, rapidity, and automation in detection provided by capillary zone electrophoresis. Cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) were detected in their purified forms as well as in the crude saps of infected Nicotiana benthamiana leaves and Oncidium orchid flowers. The two orchid virus-antibody complexes were resolved via the combined actions of electrophoretic migration and electro-osmotic flow along a buffer-filled, uncoated fused-silica capillary. The I-CZE fractions collected from both CymMV- and ORSV-antibody complex peaks, as well as the RNA purified from them, retained their infectivity upon inoculation onto Chenopodium quinoa. I-CZE assays were able to detect as little as 10 fg each of both CymMV and ORSV in their purified forms as well as in the crude saps of infected N. benthamiana and Oncidium orchid. As multiple samples can be analyzed rapidly, I-CZE offers an ideal diagnostic technique for routine mass-indexing programs such as virus-free certification, breeding for virus-resistant cultivars, plant quarantine, and germ plasm screening. This is the first report of the application of I-CZE for the detection of plant viruses.

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