scholarly journals PREVENTION OF PROTEIN ADSORPTION ON BARE FUSED-SILICA CAPILLARY BY PEG IN CAPILLARY ZONE ELECTROPHORESIS

2010 ◽  
Vol 9 (3) ◽  
pp. 410-413
Author(s):  
Adhitasari Suratman ◽  
Hermann Waetzig
Keyword(s):  
Protein Adsorption ◽  
Capillary Zone Electrophoresis ◽  
Protein Separation ◽  
Fused Silica ◽  
Buffer Solution ◽  
Relative Standard ◽  
Zone Electrophoresis ◽  
Fused Silica Capillary ◽  
Capillary Zone ◽  
Silica Capillary

The protein separation was studied in capillary zone electrophoresis for preventing protein adsorption on the capillary wall. ß-lactoglobulin (pI: 4.83-5.4, Mr: 18.4 kDa), cytochrome c (pI: 9.59, Mr: 11.7 kDa) and ß-casein (pI: 4.6, Mr: 24 kDa) were used as protein models. Strong adsorption of the proteins occurred onto the capillary at a pH around their pIs. In order to prevent protein adsorption, PEG (Poly(ethylene glycol)) was investigated as an effective substance to stabilize the proteins native state and coat the bare fused-silica capillary surface. The presence of 32 mg/mL PEG in buffer solution in a pH range of 6.0 to 4.0 was successful to suppress protein adsorption during the separation. It can also be confirmed with the reproducibility of apparent EOF mobility with percentile RSD (Relative Standard Deviation) less than 2% in long-term measurement.   Keywords: PEG, protein adsorption, CZE

scholarly journals Determination of Chlorinated Phenols in Water and Soil by Capillary Zone Electrophoresis

1997 ◽  
Vol 80 (6) ◽  
pp. 1308-1314 ◽  
Author(s):  
Wayne E Rae ◽  
Charles A Lucy
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Fused Silica ◽  
Soil Samples ◽  
Chlorinated Phenols ◽  
Relative Standard ◽  
Zone Electrophoresis ◽  
Water And Soil Samples ◽  
Fused Silica Capillary ◽  
Capillary Zone

Abstract A capillary zone electrophoresis (CZE) method was developed to separate and determine chlorinated phenols in water and soil samples. A mixture of 16 chlorinated phenols was resolved in 25 min by using a 77 cm (70 cm to detector) × 75 μm fused silica capillary with 0.015M tetraborate/0.045M phosphate (pH 7.3) buffer at 22 kV. Calibration linearities for water samples in the low parts-permillion range were good (correlation coefficient > 0.99) for all solutes except p-chlorophenol. Average precision was 17% relative standard deviation. Typical detection limits were in the 200 μg/L range. Recoveries of chlorinated phenols from synthetic soil samples with methanol were quantitative.

scholarly journals Detection of Cymbidium Mosaic Potexvirus and Odontoglossum Ringspot Tobamovirus Using Immuno-Capillary Zone Electrophoresis

1999 ◽  
Vol 89 (6) ◽  
pp. 522-528 ◽  
Author(s):  
Alvin Jin-Cherng Eun ◽  
Sek-Man Wong
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Chenopodium Quinoa ◽  
Diagnostic Technique ◽  
Plant Viruses ◽  
Fused Silica ◽  
Zone Electrophoresis ◽  
Fused Silica Capillary ◽  
Capillary Zone ◽  
Antibody Complex ◽  
Multiple Samples

Immuno-capillary zone electrophoresis (I-CZE) is a technique that combines the specificity afforded by serological assays with the sensitivity, rapidity, and automation in detection provided by capillary zone electrophoresis. Cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) were detected in their purified forms as well as in the crude saps of infected Nicotiana benthamiana leaves and Oncidium orchid flowers. The two orchid virus-antibody complexes were resolved via the combined actions of electrophoretic migration and electro-osmotic flow along a buffer-filled, uncoated fused-silica capillary. The I-CZE fractions collected from both CymMV- and ORSV-antibody complex peaks, as well as the RNA purified from them, retained their infectivity upon inoculation onto Chenopodium quinoa. I-CZE assays were able to detect as little as 10 fg each of both CymMV and ORSV in their purified forms as well as in the crude saps of infected N. benthamiana and Oncidium orchid. As multiple samples can be analyzed rapidly, I-CZE offers an ideal diagnostic technique for routine mass-indexing programs such as virus-free certification, breeding for virus-resistant cultivars, plant quarantine, and germ plasm screening. This is the first report of the application of I-CZE for the detection of plant viruses.

scholarly journals Simultaneous Separation of 14 Antiarrhythmic Drugs and Determination of Mexiletine and Flecainide by Capillary Zone Electrophoresis

2007 ◽  
Vol 90 (4) ◽  
pp. 977-986 ◽  
Author(s):  
Rafal Pietra ◽  
Dorota Kowalczuk ◽  
Hanna Hopkala
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Applied Voltage ◽  
Antiarrhythmic Drugs ◽  
Fused Silica ◽  
Effective Length ◽  
Relative Standard ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone

Abstract A method using capillary zone electrophoresis was developed for the simultaneous separation of 14 antiarrhythmic drugs belonging to various classes. The drugs are separated on a fused-silica capillary, 90 cm 75 m (72 cm effective length), with phosphate and acetate buffers as background electrolytes and UV detection at 217 nm. The effects of buffer pH, temperature, and applied voltage on the migration of the drugs were studied. The pH was found to be the most significant factor determining effective separation. The antiarrhythmic compounds are completely separated within a relatively short time (<7 min) by using 70 mM phosphate buffer at pH 7.91, an applied voltage of 28 kV, and a temperature of 32C. Mexiletine (MEX) and flecainide (FLE) were quantified under conditions of the optimum separation. The calibration graphs were constructed over the concentration range of 4.014.0 g/mL for both drugs with good correlation (r 0.9999). Detection and quantitation limits were found to be 0.5 and 1.5 g/mL for FLE and 0.7 and 2.1 g/mL for MEX, respectively. The proposed method was used for the determination of both drugs in their commercial forms with satisfactory precision (relative standard deviations of 0.361.21% for FLE and 0.781.66% for MEX) and accuracy (relative standard errors of 0.131.17% for FLE and 0.351.18% for MEX).

Use of quasi-isoelectric buffers to limit protein adsorption in capillary zone electrophoresis

2008 ◽  
Vol 29 (15) ◽  
pp. 3164-3167 ◽  
Author(s):  
Martine Poitevin ◽  
Karim Hammad ◽  
Ichraf Ayed ◽  
Pier Giorgio Righetti ◽  
Gabriel Peltre ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone
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