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An improved capillary isoelectric focusing-mass spectrometry method for high-resolution characterization of monoclonal antibody charge variants

2022 ◽  
Author(s):  
Tian Xu ◽  
Linjie Han ◽  
Alayna M. George Thompson ◽  
Liangliang Sun
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
High Resolution ◽  
Isoelectric Focusing ◽  
Capillary Isoelectric Focusing ◽  
Spectrometry Method ◽  
Mass Spectrometry Method ◽  
Charge Variants

Routine and high-resolution characterization of monoclonal antibody (mAb) charge variants is vital for controlling mAb quality as therapeutics. Capillary isoelectric focusing-mass spectrometry (cIEF-MS) has emerged as a powerful tool for...

Sample displacement chromatography of monoclonal antibody charge variants and aggregates

2021 ◽  
Author(s):  
Urh Černigoj ◽  
Blaž Nemec ◽  
Aleš Štrancar
Keyword(s):  
Monoclonal Antibody ◽  
Displacement Chromatography ◽  
Charge Variants

Separation of charge variants of a monoclonal antibody by overloaded ion exchange chromatography

2021 ◽  
Vol 1658 ◽  
pp. 462607
Author(s):  
Krystian Baran ◽  
Patrycja Zimoch ◽  
Artur Stańczak ◽  
Wojciech Piątkowski ◽  
Dorota Antos
Keyword(s):  
Monoclonal Antibody ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Charge Variants

Ultra-short ion-exchange columns for fast charge variants analysis of therapeutic proteins

2021 ◽  
pp. 462568
Author(s):  
Jose Antonio Navarro-Huerta ◽  
Amarande Murisier ◽  
Jennifer M. Nguyen ◽  
Matthew A. Lauber ◽  
Alain Beck ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Therapeutic Proteins ◽  
Fast Charge ◽  
Charge Variants

scholarly journals Similarity demonstrated between isolated charge variants of MB02, a biosimilar of bevacizumab, and Avastin® following extended physicochemical and functional characterization

Biologicals ◽  
2021 ◽  
Author(s):  
Isabel Ruppen ◽  
Marie-Elise Beydon ◽  
Clea Solís ◽  
Daniel Sacristán ◽  
Isabel Vandenheede ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Charge Variants

Inter-laboratory Study to Evaluate the Performance of Automated Online Characterization of Antibody Charge Variants by Multi-Dimensional LC-MS/MS

Talanta ◽  
2021 ◽  
pp. 122628
Author(s):  
Julien Camperi ◽  
Ingrid Grunert ◽  
Katrin Heinrich ◽  
Martin Winter ◽  
Saban Oezipek ◽  
...  
Keyword(s):  
Laboratory Study ◽  
Charge Variants

scholarly journals A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants

2021 ◽  
Author(s):  
Shu‐Ying Jing ◽  
Ce Shi ◽  
Hui Yi Leong ◽  
Jun‐Jie Yuan ◽  
Dong Gao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Continuous Chromatography ◽  
Charge Variants

Multiattribute Monitoring of Antibody Charge Variants by Cation-Exchange Chromatography Coupled to Native Mass Spectrometry

2021 ◽  
Author(s):  
Markus Haberger ◽  
Anna-Katharina Heidenreich ◽  
Michaela Hook ◽  
Jürgen Fichtl ◽  
Rainer Lang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cation Exchange ◽  
Native Mass Spectrometry ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Charge Variants

Using Continuous Chromatography Methodology to Achieve High-Productivity and High-Purity Enrichment of Charge Variants for Analytical Characterization

2021 ◽  
pp. 462008
Author(s):  
Elizabeth Bigelow ◽  
Yuanli Song ◽  
Jie Chen ◽  
Melissa Holstein ◽  
Yunping Huang ◽  
...  
Keyword(s):  
High Purity ◽  
Analytical Characterization ◽  
High Productivity ◽  
Continuous Chromatography ◽  
Charge Variants
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