scholarly journals Live imaging of mouse embryos during pre-implantation and peri-implantation development

2019 ◽  
Author(s):  
Neophytos Christodoulou ◽  
Antonia Weberling ◽  
Magdalena Zernicka-Goetz
Keyword(s):  
Single Cell ◽  
Embryo Development ◽  
Cell Tracking ◽  
Live Imaging ◽  
Mouse Embryos ◽  
Pharmacological Manipulations

Abstract Live visualisation of embryo development is a powerful tool for scientists to understand how morphogenetic events shape the embryo. Here, we report on a culturing technique that allows live imaging of pre- and peri-implantation mouse embryos throughout the process of blastocyst to egg cylinder transition, enabling single cell tracking and delineation of the tissue dynamics accompanying this morphogenetic stage. At the same time, this protocol can be used for pharmacological manipulations of mouse embryos.

scholarly journals Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

10.3791/56291 ◽  
2017 ◽  
Author(s):  
Rosa Gómez-Villafuertes ◽  
Lucía Paniagua-Herranz ◽  
Sergio Gascon ◽  
David de Agustín-Durán ◽  
María de la O Ferreras ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Biology ◽  
Cell Tracking ◽  
Live Imaging ◽  
Neural Populations

scholarly journals Optical and genetic tools for in vivo single cell tracking

2021 ◽  
Vol 358 ◽  
pp. 109192
Author(s):  
Yajie Liang ◽  
Liset M. de la Prida
Keyword(s):  
Single Cell ◽  
Cell Tracking ◽  
Genetic Tools

scholarly journals Single cell tracking based on Voronoi partition via stable matching

2020 ◽  
Author(s):  
Young Hwan Chang ◽  
Jeremy Linsley ◽  
Josh Lamstein ◽  
Jaslin Kalra ◽  
Irina Epstein ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Tracking ◽  
Stable Matching ◽  
Voronoi Partition

scholarly journals Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives

2009 ◽  
Vol 27 (5) ◽  
pp. 266-276 ◽  
Author(s):  
Sonja Nowotschin ◽  
Guy S. Eakin ◽  
Anna-Katerina Hadjantonakis
Keyword(s):  
Fluorescent Proteins ◽  
Live Imaging ◽  
Mouse Embryos

Software tools for single-cell tracking and quantification of cellular and molecular properties

2016 ◽  
Vol 34 (7) ◽  
pp. 703-706 ◽  
Author(s):  
Oliver Hilsenbeck ◽  
Michael Schwarzfischer ◽  
Stavroula Skylaki ◽  
Bernhard Schauberger ◽  
Philipp S Hoppe ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Tracking ◽  
Software Tools ◽  
Molecular Properties

scholarly journals Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Maria Vera-Rodriguez ◽  
Shawn L. Chavez ◽  
Carmen Rubio ◽  
Renee A. Reijo Pera ◽  
Carlos Simon
Keyword(s):  
Prediction Model ◽  
Single Cell ◽  
Embryo Development ◽  
Human Embryo ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Human Embryo Development ◽  
Early Human

scholarly journals Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells – Tools for analyzing dynamics of cell cycle, migration, and lineage selection

Methods ◽  
2018 ◽  
Vol 133 ◽  
pp. 81-90 ◽  
Author(s):  
Katja M. Piltti ◽  
Brian J. Cummings ◽  
Krystal Carta ◽  
Ayla Manughian-Peter ◽  
Colleen L. Worne ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Cell Tracking ◽  
Time Lapse ◽  
Live Cell ◽  
Time Lapse Imaging

scholarly journals Quantitative single-cell live imaging links HES5 dynamics with cell-state and fate in murine neurogenesis

2018 ◽  
Author(s):  
Cerys S Manning ◽  
Veronica Biga ◽  
James Boyd ◽  
Jochen Kursawe ◽  
Bodvar Ymisson ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Fate ◽  
Live Imaging ◽  
Protein Quantification ◽  
Expression Change ◽  
Dynamic View ◽  
Transient Period ◽  
Gene Expression Dynamics ◽  
Transcription Factor Expression ◽  
Protein Fluctuations

AbstractDuring embryogenesis cells make fate decisions within complex tissue environments. The levels and dynamics of transcription factor expression regulate these decisions. Here we use single cell live imaging of an endogenous HES5 reporter and absolute protein quantification to gain a dynamic view of neurogenesis in the embryonic mammalian spinal cord. We report that dividing neural progenitors show both aperiodic and periodic HES5 protein fluctuations. Mathematical modelling suggests that in progenitor cells the HES5 oscillator operates close to its bifurcation boundary where stochastic conversions between dynamics are possible. HES5 expression becomes more frequently periodic as cells transition to differentiation which, coupled with an overall decline in HES5 expression, creates a transient period of oscillations with higher fold expression change. This increases the decoding capacity of HES5 oscillations and correlates with interneuron versus motor neuron cell fate. Thus, HES5 undergoes complex changes in gene expression dynamics as cells differentiate.

Sign in / Sign up

Export Citation Format

Share Document